Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China
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Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of post-weaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather’s sugar flotation technique and microscopy at×400 magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.
Abstract
Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of post-weaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather’s sugar flotation technique and microscopy at×400 magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.
참고문헌 (42)
- Jex AR, "Classification of Cryptosporidium species from patients with sporadic cryptosporidiosis by use of sequence-based multilocus analysis following mutation scanning", J Clin Microbiol, 46, pp.2252~2262 (2008)
- Tomazic ML, "Molecular characterization of Cryptosporidium isolates from calves in Argentina", Vet Parasitol, 198, pp.382~386 (2013)
- Xiao L, "Molecular epidemiology of Cryptosporidiosis: an update", Exp Parasitol, 124, pp.80~89 (2010)
- Leoni F, "Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000", J Med Microbiol, 55, pp.703~707 (2006)
- Kváč M, "Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa)", Vet Parasitol, 191, pp.218~227 (2013)
- Yui T, "Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre- and post-weaned piglets and adult pigs in Japan", Parasitol Res, 113, pp.359~365 (2014)
- Sheoran A, "Infection with Cryptosporidium hominis provides incomplete protection of the host against Cryptosporidium parvum", J Infect Dis, 205, pp.1019~1023 (2012)
- Akiyoshi DE, "Characterization of Cryptosporidium meleagridis of human origin passaged through different host species", Infect Immun, 71, pp.1828~1832 (2003)
- Darabus G, "The homologous and interspecies transmission of Cryptosporidium parvum and Cryptosporidium meleagridis", Pol J Vet Sci, 6, pp.225~228 (2003)
- Bouzid M, "Cryptosporidium pathogenicity and virulence", Clin Microbiol Rev, 26, pp.115~134 (2013)
- Omidian Z, "Application of recombinant Cryptosporidium parvum P23 for isolation and prevention", Parasitol Res, 113, pp.229~237 (2014)
- Kvác M, "Cryptosporidium pig genotype II in immunocompetent man", Emerg Infect Dis, 15, pp.982~983 (2009)
- Xiao L, "Identification of the Cryptosporidium pig genotype in a human patient", J Infect Dis, 185, pp.1846~1848 (2002)
- Qiu SX, "The prevalence of Cryptosporidium spp. in pigs in Henan province", Chin J Zoonosis, 24, pp.481~482 (2008)
- Zhao C, "Epidemiologic survey of pig cryptosporidiosis in Fengtai county in Anhui province", Chin J Vet Parasitol, 11, pp.42~44 (2003)
- Chen F, "Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China", Zoonoses Public Health, 54, pp.393~400 (2007)
- Xiao L, "Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater", Appl Environ Microbiol, 67, pp.1097~1101 (2001)
- Burland TG, "DNASTAR's Lasergene sequence analysis software", Methods Mol Biol, 132, pp.71~91 (2000)
- Tamura K, "MEGA 4: molecular evolutionary genetics analysis (MEGA) software version 4.0", Mol Biol Evol, 24, pp.1596~1599 (2007)
- Wang R, "Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China", Parasitol Res, 107, pp.1489~1494 (2010)
- Zhao CC, "Epidemiologic survey on pig cryptosporidiosis in Fengtai county in Anhui province", Chin J Vet Parasitol, 11, pp.42~44 (2003)
- Ryan UM, "Identification of a novel Cryptosporidium genotype in pigs", Appl Environ Microbiol, 69, pp.3970~3974 (2003)
- Maddox-Hyttel C, "Cryptosporidium and Giardia in different age groups of Danish cattle and pigs-occurrence and management associated risk factors", Vet Parasitol, 141, pp.48~59 (2006)
- Suárez-Luengas L, "Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain)", Vet Parasitol, 148, pp.231~235 (2007)
- Zintl A, "Prevalence of Cryptosporidium species in intensively farmed pigs in Ireland", Parasitology, 134, pp.1575~1582 (2007)
- Nguyen ST, "Prevalence and risk factors associated with Cryptosporidium oocyst shedding in pigs in Central Vietnam", Res Vet Sci, 93, pp.848~852 (2012)
- Wieler LH, "Prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern Germany", J Vet Med B Infect Dis Vet Public Health, 48, pp.151~159 (2001)
- Roepstorff A, "Epidemiology and control of helminth infections in pigs under intensive and non-intensive production systems", Vet Parasitol, 54, pp.69~85 (1994)
- Damriyasa IM, "Prevalence and age-dependent occurrence of intestinal protozoan infections in suckling piglets", Berl Münch Tierärztl Wochenschr, 119, pp.287~290 (2006)
- Zhang W, "Prevalence and genetic characterizations of Cryptosporidium spp. in pre-weaned and post-weaned piglets in Heilongjiang Province, China", PLoS One, 8, pp.e67564~ (2013)
- Morgan UM, "Molecular and biological characterisation of Cryptosporidium in pigs", Australian Vet J, 77, pp.44~47 (1999)
- Guselle NJ, "Biology of Cryptosporidium parvum in pigs: from weaning to market", Vet Parasitol, 113, pp.7~18 (2003)
- Ryan UM, "Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa)", J Parasitol, 90, pp.769~773 (2004)
- Langkjaer RB, "Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark", Parasitology, 134, pp.339~350 (2007)
- Hsu BM, "Detection and species identification of Cryptosporidium from Taiwan feeding animals", J Parasitol, 94, pp.252~256 (2008)
- Leoni F, "Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000", J Med Microbiol, 55, pp.703~707 (2006)
- Kvác M, "Prevalence and age-related infection of Cryptosporidium suis, C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic", Vet Parasitol, 160, pp.319~322 (2009)
- Xiao S, "Occurrences and genotypes of Cryptosporidium oocysts in river network of southern-eastern China", Parasitol Res, 110, pp.1701~1709 (2012)
- Feng Y, "Cryptosporidium genotype and subtype distribution in raw wastewater in Shanghai, China: evidence for possible unique Cryptosporidium hominis transmission", J Clin Microbiol, 47, pp.153~157 (2009)
- Feng Y, "Occurrence, source, and human infection potential of Cryptosporidium and Giardia spp. in source and tap water in Shanghai, China", Appl Environ Microbiol, 77, pp.3609~3616 (2011)
- Jeníková M, "New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II", Vet Parasitol, 176, pp.120~125 (2011)
- Yoshiuchi R, "Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan", Vet Parasitol, 174, pp.313~316 (2010)