미선나무 잎과 줄기의 성분 분석 및 안전성 평가
Analysis on the Components and Safety Evaluation of Abeliophyllum distichum Nakai Leaves and Stems
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Objectives: This study was carried out in order to analyze the composition of the leaves and stems ofAbeliophyllum distichum Nakai, with the aim of obtaining basic data for utilizing the plant as a food ingredient,as well as for processing. Methods: Leaves and stems from Abeliophyllum distichum Nakai were harvested at Cheongcheon-myeon,Geosan-gun, Chungcheongbuk-do, and were subsequently freeze-dried and ground to a fine powder for chemicalcomponent analysis and safety evaluation. Results: The moisture contents of Abeliophyllum distichum Nakai leaves and stems were respectively 65.07%and 40.97%, and the crude ash contents were 1.32% and 0.91%. In addition, the crude protein contents were11.97% and 3.77%, and the crude fat contents were 2.52% and 0.36%, respectively. The fructose and glucosecontents were 32.13 mg/g and 56.17 mg/g for leaves, and 11.38 mg/g and 10.59 mg/g for stems. The major fattyacids of the leaves were palmitic acid (31.79%) and stearic acid (14.79%), and those for stems were linolenicacid (32.78%) and palmitic acid (26.75%). The ascorbic acid contents of leaves and stems were 1.32 mg/g and0.30 mg/g respectively. The calcium content was found to be the highest among the minerals tested, both in theleaves and stems, with the levels being 166.17 mg/100 g for leaves and 592.34 mg/100 g for stems. The contentof organic acid was greater in leaves than in stems, with that of malic acid accounting for more than 75% oftotal organic acids for both samples. The total phenolic compounds and flavonoid contents of Abeliophyllumdistichum Nakai were 50.64 mg/g and 13.53 mg/g in leaves and 96.47 mg/g and 18.53 mg/g in stems,respectively. No changes were shown in the number of micronucleated polychromatic erythrocytes (MNPCE)among 2,000 polychromatic erythrocytes compared to the negative control. Abeliophyllum distichum Nakai wasadministered orally to rats in order to investigate acute toxicity. The LD50 values in rats were above 2,000 mg/kg. Conclusion: These results indicate that the leaves and stems of Abeliophyllum distichum Nakai can be used asnatural ingredients in the development of nutritional and functional materials.
Abstract
Objectives: This study was carried out in order to analyze the composition of the leaves and stems ofAbeliophyllum distichum Nakai, with the aim of obtaining basic data for utilizing the plant as a food ingredient,as well as for processing. Methods: Leaves and stems from Abeliophyllum distichum Nakai were harvested at Cheongcheon-myeon,Geosan-gun, Chungcheongbuk-do, and were subsequently freeze-dried and ground to a fine powder for chemicalcomponent analysis and safety evaluation. Results: The moisture contents of Abeliophyllum distichum Nakai leaves and stems were respectively 65.07%and 40.97%, and the crude ash contents were 1.32% and 0.91%. In addition, the crude protein contents were11.97% and 3.77%, and the crude fat contents were 2.52% and 0.36%, respectively. The fructose and glucosecontents were 32.13 mg/g and 56.17 mg/g for leaves, and 11.38 mg/g and 10.59 mg/g for stems. The major fattyacids of the leaves were palmitic acid (31.79%) and stearic acid (14.79%), and those for stems were linolenicacid (32.78%) and palmitic acid (26.75%). The ascorbic acid contents of leaves and stems were 1.32 mg/g and0.30 mg/g respectively. The calcium content was found to be the highest among the minerals tested, both in theleaves and stems, with the levels being 166.17 mg/100 g for leaves and 592.34 mg/100 g for stems. The contentof organic acid was greater in leaves than in stems, with that of malic acid accounting for more than 75% oftotal organic acids for both samples. The total phenolic compounds and flavonoid contents of Abeliophyllumdistichum Nakai were 50.64 mg/g and 13.53 mg/g in leaves and 96.47 mg/g and 18.53 mg/g in stems,respectively. No changes were shown in the number of micronucleated polychromatic erythrocytes (MNPCE)among 2,000 polychromatic erythrocytes compared to the negative control. Abeliophyllum distichum Nakai wasadministered orally to rats in order to investigate acute toxicity. The LD50 values in rats were above 2,000 mg/kg. Conclusion: These results indicate that the leaves and stems of Abeliophyllum distichum Nakai can be used asnatural ingredients in the development of nutritional and functional materials.
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