햄스터 볼주머니를 이용한 구강점막 자극평가 조건설정
Condition Setting for Oral Mucosal Irritation Evaluation using Hamster Cheek Pouch
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Objectives: The purpose of this study is to evaluate the optimum conditions for oral mucosal irritation testingusing the buccal pouch of hamsters. Methods: Test materials were applied to the buccal pouch of seven-week old male Syrian hamsters (SLC, Japan)four times at one-hour intervals and macroscopic changes were examined at 24 hours after final treatment. Aftersacrifice, the buccal pouches were removed and prepared for histopathological evaluation. In order to set theexposure time, we performed exposure tests of 5, 12, 18 and 23 minutes using sodium lauryl sulfate (SLS) 1%and set the treatment volume from the test results at 2, 3, or 4 ml treatment using SLS 1%, Triton X-100 1%and ethanol. After setting the experimental conditions, seven groups of materials [sodium lauryl sulfate (SLS)(1%), Triton X-100 (1%), hydrogen peroxide (3%), ethanol (100%), chlorhexidine (0.2%, 2%), phosphate buffersaline (PBS)] were assessed. Results: Experimental conditions of material exposure time were fixed as 18 minutes from the exposure testsof 5, 12, 18 or 23 min using sodium lauryl sulfate (SLS) 1%. Treated volume was set as 4 ml per each pouchfrom the test results of 2, 3, or 4 ml treatments using SLS 1%, Triton X-100 1% and ethanol. The results in termsof irritation degree were in the order of sodium lauryl sulfate (SLS) (1%) > Triton X-100 (1%) ≒ hydrogenperoxide (3%) > ethanol (100%) ≒ chlorhexidine (0.2%, 2%) > phosphate buffer saline (PBS). Conclusion: From this study, suitable conditions for hamster mucosal irritation testing were suggested and thismethod was verified through materials commonly used on oral mucosal membranes.
Abstract
Objectives: The purpose of this study is to evaluate the optimum conditions for oral mucosal irritation testingusing the buccal pouch of hamsters. Methods: Test materials were applied to the buccal pouch of seven-week old male Syrian hamsters (SLC, Japan)four times at one-hour intervals and macroscopic changes were examined at 24 hours after final treatment. Aftersacrifice, the buccal pouches were removed and prepared for histopathological evaluation. In order to set theexposure time, we performed exposure tests of 5, 12, 18 and 23 minutes using sodium lauryl sulfate (SLS) 1%and set the treatment volume from the test results at 2, 3, or 4 ml treatment using SLS 1%, Triton X-100 1%and ethanol. After setting the experimental conditions, seven groups of materials [sodium lauryl sulfate (SLS)(1%), Triton X-100 (1%), hydrogen peroxide (3%), ethanol (100%), chlorhexidine (0.2%, 2%), phosphate buffersaline (PBS)] were assessed. Results: Experimental conditions of material exposure time were fixed as 18 minutes from the exposure testsof 5, 12, 18 or 23 min using sodium lauryl sulfate (SLS) 1%. Treated volume was set as 4 ml per each pouchfrom the test results of 2, 3, or 4 ml treatments using SLS 1%, Triton X-100 1% and ethanol. The results in termsof irritation degree were in the order of sodium lauryl sulfate (SLS) (1%) > Triton X-100 (1%) ≒ hydrogenperoxide (3%) > ethanol (100%) ≒ chlorhexidine (0.2%, 2%) > phosphate buffer saline (PBS). Conclusion: From this study, suitable conditions for hamster mucosal irritation testing were suggested and thismethod was verified through materials commonly used on oral mucosal membranes.
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