광주지역내 바닥분수 재이용수에서 분리한 장구균의 항생제 내성 연구
A Study of Antibiotic Resistance of Enterococci Isolated from Recycling Water of Floor Fountains in Gwangju Area
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives: This study aims to evaluate water quality in terms of microorganisms and identify the antibiotic resistance of Enterococci isolated from the recycling water in floor fountains at three parks and one reservoir in the Gwangju area. Methods: Water samples were analyzed for Enterococci using membrane Enterococcus indoxyl β d glucoside agar (mEI) as described in USEPA Method 1600. The vancomycin-resistant Enterococci with VanA and VanB were identified by PCR. An examination of the antibiotic resistance of isolates against 14 antibiotics was performed by the disk diffusion method. Results: The drinking water quality criterion was exceeded for total colony counts in 68% of all recycling water samples. The average concentration of total califorms and fecal coliforms was 139,325 and 413 CFU/100 mL, respectively. VanA and VanB were not detected from the isolates. We found the antibiotic resistant Enterococci strains to be E. faecalis, E. faecium, E. durans, E. mundtii, E. hirae, and E. thailandicus. The isolates were resistant to Rifampin (50%), Erythromycin (25.8%), Tetracycline (10.2%), Nitrofurantoin (8.1%), Minocycline (3.1%), Erythromycin (1.2%), Penicillin (0.7%), Norfloxacin (0.5%), and Teicoplanin (0.5%) among the 14 antibiotics tested. Antibiotic resistance tests for Enterococci from the recycling water of floor fountains resulted in 30.2% showing resistance to two or more antibiotics. Conclusions: These results showed that the multi-antibiotic resistance of Enterococci, E. coli, and others should be investigated continuously in each environment field.
Abstract
Objectives: This study aims to evaluate water quality in terms of microorganisms and identify the antibiotic resistance of Enterococci isolated from the recycling water in floor fountains at three parks and one reservoir in the Gwangju area. Methods: Water samples were analyzed for Enterococci using membrane Enterococcus indoxyl β d glucoside agar (mEI) as described in USEPA Method 1600. The vancomycin-resistant Enterococci with VanA and VanB were identified by PCR. An examination of the antibiotic resistance of isolates against 14 antibiotics was performed by the disk diffusion method. Results: The drinking water quality criterion was exceeded for total colony counts in 68% of all recycling water samples. The average concentration of total califorms and fecal coliforms was 139,325 and 413 CFU/100 mL, respectively. VanA and VanB were not detected from the isolates. We found the antibiotic resistant Enterococci strains to be E. faecalis, E. faecium, E. durans, E. mundtii, E. hirae, and E. thailandicus. The isolates were resistant to Rifampin (50%), Erythromycin (25.8%), Tetracycline (10.2%), Nitrofurantoin (8.1%), Minocycline (3.1%), Erythromycin (1.2%), Penicillin (0.7%), Norfloxacin (0.5%), and Teicoplanin (0.5%) among the 14 antibiotics tested. Antibiotic resistance tests for Enterococci from the recycling water of floor fountains resulted in 30.2% showing resistance to two or more antibiotics. Conclusions: These results showed that the multi-antibiotic resistance of Enterococci, E. coli, and others should be investigated continuously in each environment field.
참고문헌 (23)
- "Water Environment Conservation Act Article 2(Definitions)", ~ (2017)
- 유영아, "서울시내 유통식품에서 분리한 대장균의 항생제 내성 및 내성유전자", 한국식품위생안전성학회지, 25(3), pp.220~225 (2010), 한국식품위생안전성학회
- Jung, D. H., "Prevalence of fluoroquinolone-resistant river water isolates of Escherichia coli", Journal of Natural Science, 17, pp.95~107 (2005), SWINS
- Jindal, A, "Antimicrobial use and resistance in swine waste treatment systems", Applied and Environmental Microbiology, 72(12), pp.7813~7820 (2006)
- Dzidic, S., "Horizontal gene transfer emerging multidrug resistance in hospital bacteria", Acta Pharmacologica Sinica, 24(6), pp.519~526 (2003)
- Lee, K. S., "Distribution of antibiotics or heavy metal resistant bacteria and requency requency of multidrug resistance in Kum river area", Korean Journal of Environmental Biology, 11(2), pp.131~144 (1993)
- Coleman, M. E, "Microbial risk assessment scenarios, causality, and uncertainty", Microbe, 2(1), pp.13~17 (2007)
- 오향균, "도시하수 및 그 주변 하천 환경 중 항생제 내성 세균 노출 특성", 대한환경공학회지, 31(3), pp.232~239 (2009), 대한환경공학회
- 송재훈, "항생제 내성의 국내 현황 및 대책", 대한내과학회지, 77(2), pp.143~151 (2009), 대한내과학회
- Leclercq R, "Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium", N Engl J Med, 319, pp.157~161 (1988)
- Uttley AH, "Vancomycin-resistant enterococci", Lancet, 1, pp.57~58 (1988)
- Centers for Disease Control and Prevention (CDC), "Nosocomial enterococci resistant to vancomycin United States, 1989-1993", MMWR Morb Mortal Wkly Rep, 42, pp.597~599 (1993)
- Murray BE, "The life and times of the Enterococcus", Clin Microbiol Rev, 3, pp.46~65 (1990)
- U.S. Environmental Protection Agency, "National Primary Drinking Water regulations", Ground Water Rule, Federal Register, 71(216), pp.65574~65660 (2006)
- Ministry of the Environment, "microbial water quality guidelines for marine and freshwater recreational area", ~ (2003)
- Global antimicrobial resistance surveillance system: manual for early implementation, "World Health Organization", World Health Organization, ~ (2015)
- Ryu S, "The new Korean action plan for containment of antimicrobial resistance", J Glob Antimicrob Resist, 8, pp.70~73 (2017)
- Maarit, N., "Antibiotic resistance among different species of fecal coliformss isolated from water samples", Applied and Environmental Microbiology, 45(1), pp.79~83 (1980)
- Moubareck, C, "Multiple antibiotic resistance gene tranfer from animal to human enterococci in the digestive tract of gnotobiotic mice", Applied and Environmental Microbiology, 47(9), pp.2993~2996 (2003)
- 윤태호, "먹는물 약수터 장구균의 검출 특성과 반코마이신 내성 현황", 대한환경공학회지, 32(10), pp.979~985 (2010), 대한환경공학회
- 김재건, "제약회사 폐수처리장 방류수 중 항생제 내성 Enterobacteria에 관한 연구", 한국환경보건학회지, 42(1), pp.34~40 (2016), 한국환경보건학회
- 권혁구, "가축 분변중의 항생제 내성균주의 분포에 관한 연구", 한국환경보건학회지, 38(02), pp.142~150 (2012), 한국환경보건학회[DOI]
- Antimicrobial Agent and Chlorine Susceptibility of E. coli Group Isolated from Natural Drinking Water in Northern Gyeongbuk Area, "C. K. Sohn", Gyeongsangbukdo Government Public Institute of Health &Environment, 20~ (2007)