제약회사 폐수처리장 방류수 중 항생제 내성 Enterobacteria에 관한 연구
Study on Antibiotic Resistant Enterobacteria in Pharmaceutical Effluent
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Objectives: This study aims to examine the concentration, diversity, and antibiotic characteristics of penicillinG resistant enterobacteria present in pharmaceutical effluent. Methods: Water sampling was performed from a pharmaceutical company in Gyeonggi-do Province, Korea inMarch 2015. Water samples were plated in triplicate on tryptic soy agar plates with 32 mg/L of penicillin G. Penicillin G resistant enterobacteria were selected from the effluent and were subjected to 16S rRNA analysisfor penicillin G resistant species determination. Identified resistant strains were tested for resistance to variousantibiotics. Results: Penicillin G resistant enterobacteria were present at 6.2% as to culturable heterotrophic bacteria. Identified penicillin G resistant enterobacteria exhibited resistance to more than 10 of the antibiotics studied. These resistant bacteria are gram negative and are closely related to pathogenic species. Conclusion: Multi-antibiotic resistant bacteria in the effluent suggest a need for disinfection and advancedoxidation processes for pharmaceutical effluents.
Abstract
Objectives: This study aims to examine the concentration, diversity, and antibiotic characteristics of penicillinG resistant enterobacteria present in pharmaceutical effluent. Methods: Water sampling was performed from a pharmaceutical company in Gyeonggi-do Province, Korea inMarch 2015. Water samples were plated in triplicate on tryptic soy agar plates with 32 mg/L of penicillin G. Penicillin G resistant enterobacteria were selected from the effluent and were subjected to 16S rRNA analysisfor penicillin G resistant species determination. Identified resistant strains were tested for resistance to variousantibiotics. Results: Penicillin G resistant enterobacteria were present at 6.2% as to culturable heterotrophic bacteria. Identified penicillin G resistant enterobacteria exhibited resistance to more than 10 of the antibiotics studied. These resistant bacteria are gram negative and are closely related to pathogenic species. Conclusion: Multi-antibiotic resistant bacteria in the effluent suggest a need for disinfection and advancedoxidation processes for pharmaceutical effluents.
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