지표미생물을 이용한 시화호 유입수의 수질평가
Evaluation of Influent Water Quality Using Indicator Microorganisms in Lake Shiwha
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Lake Shiwha, the artificial lake located near the metropolitan Seoul, offers unique water environment and has been suspected for high levels of chemical and microbiological contaminations. The Lake Shiwha was originally sea but currently consists of four major inflowing surface water from agricultural, municipal, industrial areas in addition to occasional inflow from the sea. The objectives of this study are to investigate the relative contribution of microbial contaminants from each of inflowing surface water and to identify appropriate microbial indicator organisms in this unique water environment. We measured the levels of microbial contaminations in four inflowing surface water. A number of microbial indicator organisms including total coliform (TC), fecal coliform (FC), E. coli, Enterococci, somatic and male-specific coliphages were analyzed. Bacterial indicator microorganisms were detected and quantified by the ColilertR, EnterolertRkit. Surface water (50l) was sampled by ViroCap™ 5" cartridge filter and analyzed by single agar layer method for detecting coliphages. The concentrations of TC, FC, E. coli, and Enterococci were 1543 CFU/100 ml~ 1.99 106 CFU/100 ml, 0 CFU/100 ml~202 CFU/100 ml, 0 CFU/100 ml~1.80 105 CFU/100 ml, 74 CFU/100 ml~3408 CFU/100 ml, respectively. The male-specific and somatic coliphages were detected in three different inflowing surface waters. Isolated E. coli and Enterococci strains were further analyzed by 16s rDNA amplification and subsequent phylogenetic analysis from Jungwang-chun, Ansan-chun, Banwol-chun and penstock of inflowing surface water. Our results indicated that the concentrations of different fecal indicator microorganisms might not be highly correlated each other. Multiple microbial indicator organisms should be used for monitoring microbial contamination and microbial source tracking methods.
Abstract
Lake Shiwha, the artificial lake located near the metropolitan Seoul, offers unique water environment and has been suspected for high levels of chemical and microbiological contaminations. The Lake Shiwha was originally sea but currently consists of four major inflowing surface water from agricultural, municipal, industrial areas in addition to occasional inflow from the sea. The objectives of this study are to investigate the relative contribution of microbial contaminants from each of inflowing surface water and to identify appropriate microbial indicator organisms in this unique water environment. We measured the levels of microbial contaminations in four inflowing surface water. A number of microbial indicator organisms including total coliform (TC), fecal coliform (FC), E. coli, Enterococci, somatic and male-specific coliphages were analyzed. Bacterial indicator microorganisms were detected and quantified by the ColilertR, EnterolertRkit. Surface water (50l) was sampled by ViroCap™ 5" cartridge filter and analyzed by single agar layer method for detecting coliphages. The concentrations of TC, FC, E. coli, and Enterococci were 1543 CFU/100 ml~ 1.99 106 CFU/100 ml, 0 CFU/100 ml~202 CFU/100 ml, 0 CFU/100 ml~1.80 105 CFU/100 ml, 74 CFU/100 ml~3408 CFU/100 ml, respectively. The male-specific and somatic coliphages were detected in three different inflowing surface waters. Isolated E. coli and Enterococci strains were further analyzed by 16s rDNA amplification and subsequent phylogenetic analysis from Jungwang-chun, Ansan-chun, Banwol-chun and penstock of inflowing surface water. Our results indicated that the concentrations of different fecal indicator microorganisms might not be highly correlated each other. Multiple microbial indicator organisms should be used for monitoring microbial contamination and microbial source tracking methods.
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