Ortho-phenylphenol을 주성분을 하는 훈증소독제의 Trichophyton mentagrophytes, Candida albicans 그리고 Aspergillus niger에 대한 살진균 효과
Fungicidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient against Trichophyton mentagrophytes, Candida albicans and Aspergillus niger
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Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenolagainst Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillusniger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant wereleft in a sealed room (25 m3) at 21±0.5oC and 60±10% relative humidity for 15 hours. Immediately after removalfrom the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were1.0×108, 1.2×108 and 5.7×107 CFU/mL, respectively. All the colony numbers on the carriers exposed to thefumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2(the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all meannumbers of test strains recovered on the control-carriers (T value) were over 106 CFU/mL. For the fungicidal effectof the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effectivefungicidal activity against T. mentagrophytes, C. albicans and A. niger.
Abstract
Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenolagainst Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillusniger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant wereleft in a sealed room (25 m3) at 21±0.5oC and 60±10% relative humidity for 15 hours. Immediately after removalfrom the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were1.0×108, 1.2×108 and 5.7×107 CFU/mL, respectively. All the colony numbers on the carriers exposed to thefumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2(the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all meannumbers of test strains recovered on the control-carriers (T value) were over 106 CFU/mL. For the fungicidal effectof the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effectivefungicidal activity against T. mentagrophytes, C. albicans and A. niger.
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