하천에서의 Oxytetracycline 내성주에 관한 연구
Study on Oxytetracycline Resistant Bacteria in the Surface Water Environment
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Objectives: This study aims to understand the concentration, diversity, and antibiotic characteristics ofoxytetracycline resistant bacteria present in a surface water environment. Methods: Water sampling was performed in Cheongmi Stream in Gyeonggi-do, Korea in February and August2014. Water samples collected from two sites were plated in triplicate on tryptic soy agar plates with 30 mg/Lof oxytetracycline. Oxytetracycline resistant bacteria were selected from surface water in Cheongmi Stream andwere subjected to 16S rDNA analysis for oxytetracycline resistant species determination. Identified resistantstrains were tested for resistance to various antibiotics. Results: Results from this study indicate that the dominant resistant organisms in this aquatic environment arefrom family Acinetobacter and family Aeromonas. As to culturable heterotrophic bacteria, Oxytetracyclineresistant bacteria were present 0.45-0.93% during winter and 0.08-0.38% during summer. Most oxytetracyclineresistant bacteria exhibited resistance to more than ten of the antibiotics studied. The diversity of oxytetracyclineresistant bacteria in winter was higher than in summer. Conclusion: Most of these resistant bacteria are Gram negative and are closely related to pathogenic species. These results suggest that increasing multi-antibiotic resistant bacteria in the surface water environment has aclose relation to the reckless use of antibiotics in livestock.
Abstract
Objectives: This study aims to understand the concentration, diversity, and antibiotic characteristics ofoxytetracycline resistant bacteria present in a surface water environment. Methods: Water sampling was performed in Cheongmi Stream in Gyeonggi-do, Korea in February and August2014. Water samples collected from two sites were plated in triplicate on tryptic soy agar plates with 30 mg/Lof oxytetracycline. Oxytetracycline resistant bacteria were selected from surface water in Cheongmi Stream andwere subjected to 16S rDNA analysis for oxytetracycline resistant species determination. Identified resistantstrains were tested for resistance to various antibiotics. Results: Results from this study indicate that the dominant resistant organisms in this aquatic environment arefrom family Acinetobacter and family Aeromonas. As to culturable heterotrophic bacteria, Oxytetracyclineresistant bacteria were present 0.45-0.93% during winter and 0.08-0.38% during summer. Most oxytetracyclineresistant bacteria exhibited resistance to more than ten of the antibiotics studied. The diversity of oxytetracyclineresistant bacteria in winter was higher than in summer. Conclusion: Most of these resistant bacteria are Gram negative and are closely related to pathogenic species. These results suggest that increasing multi-antibiotic resistant bacteria in the surface water environment has aclose relation to the reckless use of antibiotics in livestock.
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