국내 서식 담수새우 새뱅이(Neocaridina denticulata)를 이용한 3,4-Dichloroaniline의 급성독성 교차시험(Ring test)
Ring Test as Acute Toxicity Test with Korean Freshwater Shrimp, Neocaridina denticulata using 3,4-Dichloroaniline
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.
Abstract
Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.
참고문헌 (49)
- Klimisch HJ, "A system-atic approach for evaluating the quality of experimental toxicological and ecotoxciological data", Regul Toxicol Pharm, 25, pp.1~5 (1997)
- European Communities (EC), "Common implementation strategy for the water frame work directive (2000", 60, European Communities (EC), pp.2011, 132~134
- Korean Standards Service Network (KSSN), "ISO", TC 147, Korean Satandars Association (KSA), ~
- Regulation for designation of research institute for testing chemical hazards, , NIER, ~, pp.National Institute of Environmental Research (NIER) announcement 2019-23 (2019)
- Standard for registration of agricultural pesticides and technical, , RDA, ~, pp.Rural Development Administration announcement 2019-14 (2019)
- 박용석, "Glucose-6-phosphate dehydrogenase를 이용한 Moina macrocopa의 중금속 독성 검정", 환경독성보건학회지, 18(4), pp.305~310 (2003), 환경독성보건학회
- Lee CW, "Culture method of Simocephalus mixtus (S. mixtus) to evaluate environment toxicity and measurement method of environment toxicity by S. mixtus" (2003), Korean Intellectual Property Office
- Study on the Korean native organism for ecological toxicity evaluation, "Ryu JS", National Institute of Environmental Research (NIER), 15~18 (2006)
- 남선화, "생태독성시험을 위한 국내 자생생물종 선별 연구: (1) 수서시험종", 대한환경공학회지, 40(1), pp.34~47 (2018), 대한환경공학회[DOI]
- 남선화, "생태독성시험을 위한 국내 자생생물 후보 목록 도출: (1) 수서시험종", 대한환경공학회지, 41(1), pp.10~23 (2019), 대한환경공학회[DOI]
- Establishment toxicity assessment system and management of hazardous chemicals, "Korea research institute of chemical technology", Ministry of Environment, ~ (1999)
- Development and standardization of ecotoxicity tests using species indigenous to Korea (?), "Ryu JS", National Institute of Environmental Research (NIER), ~ (2007)
- Development and standardization of ecotoxicity tests using species indigenous to Korea (?), "Ryu JS", National Institute of Environmental Research (NIER), ~ (2008)
- Development and standardization of ecotoxicity tests using species indigenous to Korea (III), "Ryu JS", National Institute of Environmental Research (NIER), ~ (2009)
- Development of biological criteria for water quality assessment using aquatic organisms (II), "Byeon MS", National Institute of Environmental Research (NIER), ~ (2009)
- Development of environmental criteria for aquatic life, "Ryu JK", National Institute of Environmental Research (NIER), ~ (2010)
- Development and standardization of ecotoxicity tests using Neocaridina denticulata (I), "Ryu JS", National Institute of Environmental Research (NIER), ~ (2010)
- Studies on biological test system for environmental hazard assessment using indigenous species, "Ryu JS", National Institute of Environmental Research (NIER), ~ (2011)
- Studies on plan for diversification of test species in ecotoxicity assessment, "Neo Environmetal Business Co", National Institute of Environmental Research (NIER), ~ (2013)
- Validation and application of acute toxicity test using Korean native freshwater species, "Par KH", National Institute of Environmental Research (NIER), ~ (2015)
- 류지성, "국내 담수새우인 새뱅이 (Neocardina denticulata)를 이용한 중금속의 급성독성시험", 환경독성보건학회지, 22(2), pp.171~175 (2007), 환경독성보건학회
- 안윤주, "수생태계 독성평가에 적용 가능한 국내 시험종 선정", 생태와 환경, 40(1), pp.1~13 (2007), 한국수생태학회
- 남선화, "국내 생물종을 이용한 생태독성평가 기반연구 : ( I ) 어류", 생태와 환경, 40(2), pp.173~183 (2007), 한국수생태학회
- 김한순, "한국산 Mallomonas caudata (Synurophyceae)의 미세구조, 핵 SSU 그리고 색소체 rbcL 유전자", 생태와 환경, 40(3), pp.387~394 (2007), 한국수생태학회
- 안윤주, "국내 생물종을 이용한 생태독성평가 기반연구: (III) 녹조류", 생태와 환경, 41(2), pp.117~127 (2008), 한국수생태학회
- 이정석, "국내산 저서 단각류를 이용한 퇴적물 독성시험법 개발에 관한 연구", 바다, 13(2), pp.147~155 (2008), 한국해양학회
- 박용석, "효소 활성을 이용한 Moina macrocopa의 중금속 독성 검정", 환경독성보건학회지, 23(1), pp.17~22 (2008), 환경독성보건학회
- 이재우, "Pentachlorophenol Sodium Salt, Potassium Dichromate, Sodium Azide에 대한 새뱅이(Neocaridina denticulata) 급성독성", 환경독성보건학회지, 25(3), pp.223~228 (2010), 환경독성보건학회
- 이재우, "Potassium dichromate에 대한 새뱅이 유생, 물벼룩 및 송사리 치어 급성독성 민감도 비교", 환경생물, 30(4), pp.314~318 (2012), 한국환경생물학회
- Development of toxicity rata and recommended water quality guideline for protection of aquatic species in national freshwater (Dissemination), "Gwangju Institute of Science and Technology (GIST)", GIST, ~ (2015)
- 김은정, "산화아연 나노물질의 미소생태계 내 거동 및 생물축적", 한국환경보건학회지, 43(3), pp.194~201 (2017), 한국환경보건학회
- 최영설, "생태독성실험을 위한 한국 우점종 유리물벼룩(Daphnia galeata)의 특성 및독성민감도: 큰물벼룩(Daphnia magna)과의 비교연구", 대한환경공학회지, 38(4), pp.193~200 (2016), 대한환경공학회[DOI]
- Cui R, "Comparative study of the sensitivity of Daphnia galeata and Daphnia magna to heavy metals", Ecotoxicol Environ Saf, 162, pp.63~70 (2018)
- Guidance document for the development of OECD guidelines for testing of chemicals (as revised in 2009), "Organization for Economic Cooperation and Development (OECD)", OECD, 27~28, pp.Organization for Economic Cooperation and Development (OECD) Series on testing and assessment No. 1 (2009)
- "National Institute of Biological Resources Home Page"[URL]
- Illustrated Flora and Fauna of Korea, "Kim HS", Ministry of education, 1~414 (1997)
- Englund RA, "The occurrence and description of Neocaridina denticulata sinensis (Kemp, 1918) (Crustacea: Decapoda: Atyide), a new introduction to the Hawaiian Island", Bishop Mus Occas Pap, 58, pp.58~65 (1999)
- Huang DJ, "Effects of the endocrine disrupter chemicals chlordane and lindane on the male green neon shrimp (Neocaridina denticulata)", Chemosphere, 57(11), pp.1621~1627 (2004)
- German Standardization Organization, "German standard methods for the examination of water, waste water and sludge-Subanimal testing (group T)-Part 6: Toxicity to fish. Determination of the non-acute poisonous effect of waste water to fish eggs by dilution limits (T 6)", Deutasches Institut fur Normung E. V. (DIN), 38415~38416 (2003)
- Validation report (Phase 1) for the zebrafish embryo toxicity test Part I, "Organization for Economic Cooperation and Development (OECD)", OECD, ~, pp.Series on testing and assessment No. 157 (2011)
- Validation report (Phase 2) for the zebrafish embryo toxicity test, "Organization for Economic Cooperation and Development (OECD)", OECD, ~, pp.Series on testing and assessment No. 179 (2012)
- "ECOTOX Knowledgebase"[URL]
- Schafer C, "Effects fo 3,4-dichloroaniline on fish populations. Comparison between R- and Kstrategists;A complete life cycle test with the guppy (Poecilia reticulata)", Arch Environ Contam Toxicolo, 21, pp.297~302 (1991)
- Chen CM, "Use of Japanese medaka (Oryzias latipes) and Tilapia (Oreochromis mossambicus) in toxicity tests on different industrial effluents in Taiwan", Arch. Environ Contam Toxicol, 40(3), pp.363~370 (2001)
- European chemicals bureau, "European union risk assessment report: 3,4-dichloroaniline(3,4-DCA)", European commision, 65~ (2006)
- Bolton S, "Pharmaceutical Statistics: Practical and clinical applications", pp.21~22 (1990), Marcel Dekker Inc
- Wang N, "Intra- and interlaboratory in acute toxicity tests with glochidia and juveniles of freshwater mussels (unionidae)", Environ Toxicol Chem, 26(10), pp.2029~2035 (2007)
- Perssone G, "Review on the acute Daphnia magna toxicity test-Evaluation of th sensitivity and the precision fo assays performed with organisms from laboratory cultures or hatched from dormant eggs", Knwl Managt Aquatic Eosyst, 393(01) (2009)
- Myriophyllum spicatum toxicity test:Results of an inter-laboratory ring test using a sediment-free test system-ring test report, "Organization for Economic Cooperation and Development (OECD)", OECD, ~, pp.Series on testing and assessment No. 205 (2014)