실험동물 랫드를 이용한 미세먼지 기도노출에 따른 호흡기계 독성에 대한 마늘의 예방효과 탐색
Preventive Effect of Garlic Administration on Respiratory Toxicity Induced through Intratracheal Instillation of Fine Dust (PM10) in Rats
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Objectives: Exposure to fine dust (PM10) could contribute to the occurrence of cardiovascular disease or respiratory abnormalities. Since garlic is known to possess an anti-oxidative stress effect, the present study was performed to evaluate the effect of garlic intake on fine dust-mediated pulmonary toxicity. Methods: Rats were intratracheally instilled with fine dust at 15 mg/kg body weight (BW)/day for five days following five-day intragastric intubation of garlic at 0.7 or 1.4 g/kgBW/day, or 13.1 mg/kgBW/day S-allyl-cysteine (SAC) as a reference component in garlic. Blood and bronchoalveolar lavage fluid (BALF) were collected. Results: Deposit of fine dust was visually and histopathologically observed in the lungs. Body weight gain during the instillation period was significantly lowered in all the groups instilled with fine dust. Neutrophil numbers in blood were significantly elevated in the fine dust alone group, but this alteration was diminished in the groups administered with garlic. Levels of serum glutathione were lower in the rats instilled with fine dust alone, and this decrease in the glutathione level seems dose-dependently compensated among the groups administered with garlic. Similar findings were observed in the BALF with statistical significance. Typical pulmonary histopathological observation related with inflammation was demonstrated in the lungs of the rats exposed to fine dust alone, whereas such histopathologic findings were not improved in the groups administered with garlic. Conclusion: The present study suggests that garlic intake could alleviate fine dust-mediated pulmonary or systemic toxicities. Further investigation is necessary to delineate the mechanism of garlic-mediated effects on pulmonary function.
Abstract
Objectives: Exposure to fine dust (PM10) could contribute to the occurrence of cardiovascular disease or respiratory abnormalities. Since garlic is known to possess an anti-oxidative stress effect, the present study was performed to evaluate the effect of garlic intake on fine dust-mediated pulmonary toxicity. Methods: Rats were intratracheally instilled with fine dust at 15 mg/kg body weight (BW)/day for five days following five-day intragastric intubation of garlic at 0.7 or 1.4 g/kgBW/day, or 13.1 mg/kgBW/day S-allyl-cysteine (SAC) as a reference component in garlic. Blood and bronchoalveolar lavage fluid (BALF) were collected. Results: Deposit of fine dust was visually and histopathologically observed in the lungs. Body weight gain during the instillation period was significantly lowered in all the groups instilled with fine dust. Neutrophil numbers in blood were significantly elevated in the fine dust alone group, but this alteration was diminished in the groups administered with garlic. Levels of serum glutathione were lower in the rats instilled with fine dust alone, and this decrease in the glutathione level seems dose-dependently compensated among the groups administered with garlic. Similar findings were observed in the BALF with statistical significance. Typical pulmonary histopathological observation related with inflammation was demonstrated in the lungs of the rats exposed to fine dust alone, whereas such histopathologic findings were not improved in the groups administered with garlic. Conclusion: The present study suggests that garlic intake could alleviate fine dust-mediated pulmonary or systemic toxicities. Further investigation is necessary to delineate the mechanism of garlic-mediated effects on pulmonary function.
참고문헌 (36)
- Dockery DW, "An association between air pollution and mortality in six U.S. cities", N Engl J Med, 329, pp.1753~1759 (1993)
- Atkinson RW, "Urban ambient particle metrics and health : A time series analysis", Epidemiology, 21, pp.501~511 (2010)
- Loomis D, "The carcinogenicity of outdoor air pollution", Lancet Oncol, 14, pp.1262~1263 (2013)
- "Development of Guideline for the preventive health damage caused by fine particle and yellow dust (Cardiovascular disease)"[URL]
- "Development of guideline for the prevention and management of particulate matter/Asian dust particles-induced adverse health effect (Pulmonary diseases)"[URL]
- Sunil VR, "World Trade Center(WTC)dust exposure in mice is associated with inflammation, oxidative stress and epigenetic changes in the lung", Exp Mol Patho, 102(1), pp.50~58 (2017)
- Anderson JO, "Clearing the air : a review of the effects of particulate matter air pollution on human health", J Med Tox, 8(2), pp.166~175 (2012)
- Miller KA, "Long-term exposure to air pollution and incidence of cardiovascular events in women", N Engl J Med, 356(5), pp.447~458 (2007)
- Qian Z, "Ambient air pollution and preterm birth : a prospective birth cohort study in Wuhan, China", Int J Hyg Environ Health, 219(2), pp.195~203 (2016)
- Bergès R, "Comparison of the chemopreventive efficacies of garlic powders with different alliin contents against aflatoxin B1 carcinogenicity in rats", Carcinogenesis, 25(10), pp.1953~1959 (2004)
- Adaki S, "Garlic : Review of literature", Indian J Cancer, 51(4), pp.577~581 (2014)
- Asemani Y, "Allium vegetables for possible future of cancer treatment", Phytother Res, 33(12), pp.3019~3039 (2019)
- Shaikh SA, "Good for the lung but bad for the liver? Garlic-induced hepatotoxicity following liver transplantation", J Clin Pharm Ther, 42(5), pp.646~648 (2017)
- Desai, G, "Onion and Garlic Intake and Breast Cancer, a Case-Control Study in Puerto Rico", Nutr Cancer, 72(5), pp.791~800 (2020)
- Hsieh, CC, "Comparing the Protection Imparted by Different Fraction Extracts of Garlic (Allium sativum L.) against Der p-Induced Allergic Airway Inflammation in Mice", Int J Mol Sci, 20(19), pp.4879~ (2019)
- Lee H, "Z-ajoene from Crushed Garlic Alleviates Cancer-Induced Skeletal Muscle Atrophy", Nutrients, 11(11), pp.2724~ (2019)
- Syed Mohammed Basheeruddin Asdaq, "Antioxidant and hypolipidemic potential of aged garlic extract and its constituent, s-allyl cysteine, in rats", Evid Based Complement Alternat Med, 2015, pp.328545~ (2015)
- American Thoracic Society, "Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease", Am J Respir Crit Care Med, 152(5 pt 2), pp.S77~S121 (1995)
- Pauwels RA, "Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: NHLBI", WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary, 2001, pp.163, 1256~1276
- Gross NJ, "Extrapulmonary effects of chronic obstructive pulmonary disease", Curr Opin Pulm Med, 7(2), pp.84~92 (2001)
- Agusti AG, "Systemic effects of chronic obstructive pulmonary disease", Eur Respir J, 21(2), pp.347~360 (2003)
- Wouters EF, "Systemic effects in COPD", Chest, 121(5 Suppl), pp.127S~130S (2002)
- "OECD Guidelines 403 for the testing of chemicals (Acute inhalation toxicity)", ~
- Osier M, "Intratracheal inhalation vs intratracheal instillation: differences in particle effects", Fundam Appl Toxicol, 40(2), pp.220~227 (1997)
- Jaffary SRA, "Acute and Sub Chronic Oral Toxicity Studies of Weight Loss Formulation in Experimental Animal Models", RADS J. Pharm. Pharm. Sci, 5(2), pp.8~12 (2017)
- Baarends EM, "Total free living energy expenditure in patients with severe chronic obstructive pulmonary disease", Am J Respir Crit Care Med, 155, pp.549~554 (1997)
- Ferreira IM, "Nutritional support for individuals with COPD : a meta-analysis", Chest, 117(3), pp.672~678 (2000)
- Brook RD, "Air pollution and cardiovascular disease : a statement for healthcare professionals from the Expert Panel on Population and Prevention Science of the American Heart Association", Circulation, 109(21), pp.2655~2671 (2004)
- Foyer CH, "Photooxidative stress in plants", Physiologia Plantarum, 92(4), pp.696~717 (1994)
- Gruhlke MC, "The human allicin-proteome : S-thioallylation of proteins by the garlic defence substance allicin and its biological effects", Free Radic Biol Med, 131, pp.144~153 (2019)
- Kim I, "Application of various cytotoxic endpoints for the toxicity prioritization of fine dust (PM2.5)sources using a multi-criteria decision-making approach", Environ Geochem Health, 42, pp.1775~1788 (2020)
- Mikkelsen L, "Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles", Nanotoxicology, 7(2), pp.117~134 (2013)
- Zhang Y, "Investigation of fine chalk dust particles’ chemical compositions and toxicities on alveolar macrophages in vitro", Chemosphere, 120, pp.500~506 (2015)
- 김현희, "S-allylcysteine의 자궁경부암세포주 HeLa에 대한 세포증식 억제효과", 생명과학회지, 25(4), pp.397~405 (2015), 한국생명과학회
- Kodera Y, "Garlic chemistry : chemical and biological properties of sulfur-containing compounds derived from garlic", Food Factors in Health Promotion and Disease Prevention, 30, pp.346~357 (2003)
- Zarezadeh M, "Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat : possible involved mechanisms", Eur J Pharmacol, 795, pp.13~21 (2017)