충남지역 다중이용시설의 환경수계에서 분리한 레지오넬라균의 특성 분석
Characterization of Legionella Isolated from the Water System at Public Facilities in Chungcheongnam-do Province
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Background: The Legionella case detection and notification rate have increased in public artificial water environments where people visit, including large buildings, public baths, and hospitals. Objectives: In this study, the distribution of Legionella and its epidemiologic characteristics were analyzed in the water systems of public facilities in Chungcheongnam-do Province in South Korea. Methods: Culture and PCR analysis were performed on 2,991 environmental water system samples collected from 2017 to 2019, and associations with year, facilities, seasons, and temperature of water system were statistically analyzed by using R-Studio for Windows. Descriptive data was compared using chi-square tests and independent t-tests. Results: The detection rate of Legionella increased from 3.1% in 2017 to 10.3% in 2019, appearing most frequently in the order of public baths, large-scale buildings, hospitals, and apartments. It was detected mainly in summer from June to August, over 1.0×103 CFU/L on average in 133 cases (66.5%). Lots of germs were detected in bathtub water, cooling tower water, and warm water (p<0.001), and it was detected at higher rates in the cities where multipurpose facilities were concentrated than in rural areas (p=0.018). Conclusions: This study suggests that continuous monitoring and control are required for Legionella in the water system environment of high risk facilities. Moreover, these results will be helpful to prepare efficient management plans to prevent the Legionellosis that occurs in Chungcheongnam-do Province.
Abstract
Background: The Legionella case detection and notification rate have increased in public artificial water environments where people visit, including large buildings, public baths, and hospitals. Objectives: In this study, the distribution of Legionella and its epidemiologic characteristics were analyzed in the water systems of public facilities in Chungcheongnam-do Province in South Korea. Methods: Culture and PCR analysis were performed on 2,991 environmental water system samples collected from 2017 to 2019, and associations with year, facilities, seasons, and temperature of water system were statistically analyzed by using R-Studio for Windows. Descriptive data was compared using chi-square tests and independent t-tests. Results: The detection rate of Legionella increased from 3.1% in 2017 to 10.3% in 2019, appearing most frequently in the order of public baths, large-scale buildings, hospitals, and apartments. It was detected mainly in summer from June to August, over 1.0×103 CFU/L on average in 133 cases (66.5%). Lots of germs were detected in bathtub water, cooling tower water, and warm water (p<0.001), and it was detected at higher rates in the cities where multipurpose facilities were concentrated than in rural areas (p=0.018). Conclusions: This study suggests that continuous monitoring and control are required for Legionella in the water system environment of high risk facilities. Moreover, these results will be helpful to prepare efficient management plans to prevent the Legionellosis that occurs in Chungcheongnam-do Province.
참고문헌 (22)
- "Infectious Disease Control and Prevention Act"[URL]
- Kim JS, "An outbreak of Legionellosis(Pontiac fever)in ICU of K hospital, Korea", Korean J Epidemiol, 7(1), pp.44~58 (1985)
- Kim MJ, "A prospective multicenter study of the etiological analysis in adults with community-acquired pneumonia : Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi", Korean J Infect Dis, 33(1), pp.24~31 (2001)
- 이인섭, "바닥분수에서 물놀이를 하는 아동들의Legionella균 감염 위해도 분석", 미생물학회지, 49(4), pp.360~368 (2013), 한국미생물학회[DOI]
- "Promoting Guidelines for Safe Recreational Water Environments/Facilities in South Korea"[URL]
- Ortiz-Roque CM, "Abundance and distribution of Legionellaceae in Puerto Rican waters", Appl Environ Microbiol, 53(9), pp.2231~2236 (1987)
- Riffard S, "Occurrence of Legionella in groundwater : an ecological study", Water Sci Technol, 43(12), pp.99~102 (2001)
- Brooks T, "Detection and identification of Legionella species from groundwaters", J Toxicol Environ Health A, 67(20-22), pp.1845~1859 (2004)
- Ko KS, "Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene(rpoB)and DotA gene(dotA)sequences", J Bacteriol, 184(8), pp.2123~2130 (2002)
- Glick TH, "Pontiac fever. An epidemic of unknown etiology in a health department: I. Clinical and epidemiologic aspects", Am J Epidemiol, 107(2), pp.149~160 (1978)
- Friedman S, "Pontiac fever outbreak associated with a cooling tower", Am J Public Health, 77(5), pp.568~572 (1987)
- Go GP, "Exposure and human risk assessment of bioaerosol", Air Clean Technol, 20(1), pp.50~56 (2007)
- Hae Kyung Lee, "Genomic Diversity of Legionella pneumophila Serogroup 1 from Environmental Water Sources and Clinical Specimens Using Pulsed-Field Gel Electrophoresis (PFGE) from 1985 to 2007, Korea", The Journal of Microbiology, 48(5), pp.547~553 (2010), 한국미생물학회
- Chien ST, "Epidemiological investigation of a case of nosocomial Legionnaires'disease in Taiwan : implications for routine environmental surveillance", Clin Microbiol Infect, 16(6), pp.761~763 (2010)
- "Management Guidelines for Legionnaires’ Diseases 2019"[URL]
- Diederen BM, "Legionella spp. and Legionnaires’ disease", J Infect, 56(1), pp.1~12 (2008)
- 이현경, "경기도내 수계시설에서 분리된 레지오넬라균의 분포현황 및 Legionella pneumophila serogroup 1의 유전학적 다양성 연구", 미생물학회지, 53(3), pp.156~162 (2017), 한국미생물학회[DOI]
- "Infectious Disease Portal"[URL]
- Kim DU, "Identification and distribution of Legionella species from environmental water sources in Chungnam, Korea", J CNIHE, 22, pp.11~24 (2012)
- Sun Hee Kim, "Surveillance of Legionella Contamination on Water Supply Systems of Public Utilizing Facilities in Gwangju, Korea", Journal of Bacteriology and Virology, 40(1), pp.19~28 (2010), 대한미생물학회
- 김진남, "레지오넬라균 출현위해도에 대한 현행 온천수 수질기준의 적합성 분석", 한국미생물·생명공학회지, 43(3), pp.259~266 (2015), 한국미생물·생명공학회[DOI]
- Bang SJ, "Indoor and outdoor distribution of Legionella spp and microbes on cooling towers water of central air conditioning facilites", Korean J Environ Health Soc, 28(3), pp.39~48 (2002)