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半支蓮의 GnRH agonist 유사활성에 의한 자궁근종 배양세포 성장억제효과

Antiproliferative effect of Scutellaria barbata D. Don. on cultured human uterine leiomyoma cells

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참고문헌

주제분류한의학

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Abstract

자궁근종은 자궁근육층의 양성 평활근세포종으로 apoptosis(細胞死)가 억제되어 과잉증식 특성을 나타낸다. 본 연구에서는 半支蓮의 자궁근종에 대한 증식저해 활성을 검정하기 위하여 배양세포의 증식억제활성을 검토하였다. 즉 사람의 자궁근종 배양세포를 사용하여 半支蓮의 자궁근종 증식저해활성을 기존의 억제인자로 알려진 gonadotrophin releasing hormone (GnRH) agonist의 하나인 buserelin과 비교실험을 통하여 해명하였다. 근종세포는 환자의 동의를 통해 자궁적출 시술후 견본으로 시험관내에서 인공배양을 하여, 배양세포에서 존재하는 GnRH receptor를 reverse-transcriptase 분석으로 검정하였다. buserelin 또는 半支蓮을 배양근종세포에 첨가한 후 72시간동안 저해활성을 검정한 결과 두 가지 약제 모두 농도의존적인 저해활성을 나타내었다.(10-5 mol/l buserelin 및 20-40 g/ml 半支蓮) 이러한 세포증식의 저해는 PKC activator인 12-O-tetradecanoylphorbor-13-acetate (TPA)에 의해 감소되었으며, PKC inhibitor인 calphostin C를 전처리 할 경우 半支蓮에 의한 증식억제 활성은 검출되지 않았다. 이러한 결과들은 buserelin과 半支蓮이 배양된 자궁근종세포에서 protein kinase C 활성화를 통한 증식억제효과를 나타내고 있음을 시사하였다. 따라서, 본 半支蓮의 작용기전이 종양세포의 세포주기의 제어에 있으므로 향후 cell cycle에 대한 연구가 필요할 것으로 보인다.

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