hCG로 유도된 자궁근종세포와 자궁평활근세포의 증식에 대한 半支蓮 억제효과
Inhibitory effect of Scutellaria barbata D. Don (Lamiaceae) onhCG-promoted proliferation of uterine leiomyomal andmyometrial smooth muscle cells
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Abstract
半支蓮(Scutellaria barbata D. Don; Lamiaceae: SB)은 중국 남부지방에 자생적으로 분포하는 다년생 본초식물로 韓醫學에서 소염제와 항암제로 쓰여져 왔다. 본 연구는 半支蓮의 자궁근종에 대한 증식억제효과와 그 기전을 해명하는데 목표를 두고 수행하였다. 半支蓮의 熱水 推出物과 정상자궁평활근세포 및 자궁근종세포를 본 실험에 사용하였다. 자궁근종은 종종 임신기간에 빠르게 커지는데 이는 hCG가 자궁근종의 세포증식에 영향을 주는 것을 의미한다. 그래서 본 실험에서는 hCG 처리된 정상자궁평활근세포 및 자궁근종세포의 과잉증식에 대한 半支蓮의 저해활동을 조사하고 근종세포의 세포주기관련 단백질(효소)의 발현에 대한 半支蓮의 영향을 검토하였다. 실험 결과 hCG는 자궁평활근세포과 자궁근종세포 양쪽 모두의 세포증식을 현저히 증가시켰으며, 半支蓮은 양쪽 세포 모두에서 hCG가 나타낸 증식효과를 대폭 감소시켰다. 한편, 半支蓮의 세포증식 억제효과는 자궁평활근세포보다 자궁근종세포에서 더 의미있게 나타났다. 또한 半支蓮은 hCG로 처리한 자궁근종세포에서 발현된 세포증식에 관련되는 단백질(효소)인 PCNA, cycline E와 cdc2의 증식도 현저히 감소시켰다. 이러한 결과는 半支蓮이 자궁근종세포에서 hCG로 유발된 세포의 과잉증식을 감소시키며, 이는 半支蓮이 자궁근종세포의 세포주기관련 단백질(효소)의 증식을 억제하는 것과 연관된 것으로 사료된다.
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