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논문KCI 등재

鷄血藤이 자궁내막암세포의 증식억제 및 세포자멸사에 미치는 영향

Growth inhibition and induction of apoptosis in endometrial cancer cell line by Spatholobus suberectus Dunn

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대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2005.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제18권 제2호|pp. 40-51

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Abstract

Purpose : This study was aimed to investigate the anti-cancer effect of the stem of Spatholobus Subrectus Dunn on the human uterine endometrial cancer cells and the mechanism of Spatholobus Subrectus Dunn on endometrial cancer cell line Methods : We counted the number of death cells treated with indicated concentration of the stem of Spatholobus Subrectus Dunn. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to see whether Spatholobus Subrectus Dunn induced anti-proliferation effect was due to apoptosis and then we observed the differential cell cycle-related gene expression by western blot analysis. Results : 1. The inhibitory effect on the growth of human endometrial cancer cell treated with the stem of Spatholobus Subrectus Dunn was increased in a concentration proportional. 2. The result of flow cytometry analysis, subG1 phase arrest was investigated 35.41% in endometrial cancer cells treated with the stem of Spatholobus Subrectus Dunn and showed the fashion of proportional concentration. 3. The gene expression of p21 and Bax protein was increased according to increasing concentration but p27, p53, CDk2, cyclin E and Bcl-2 were not exchanged. 4. The character of apoptosis, DNA fragmentation was significantly observed the fashion of proportional concentration. 5. The expression of pro-caspase3 and PARP were decreased dependent on treatment concentration. Conclusion : This study showed that the stem of Spatholobus Subrectus Dunn have the inhibitory effect on the proliferation of human uterine endometrial cancer cell and the effect was related with apoptosis.

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관련 논문AI추천

鷄血藤이 자궁경부암세포의 세포자멸사 유도와 관련 유전자 발현에 미치는 영향김병회, 백승희대한한방부인과학회 · 대한한방부인과학회지2005 · 제18권 제1호鷄血藤 추출물이 Jurkat T 임파구의 세포고사 및 세포주기 억제에 미치는 효과이인, 나헌식, 문병순, 송영준, 이계승, 전병훈, 정우철, 조남수한의병리학회 · 동의생리병리학회지2004 · 제18권 제1호鷄血藤이 子宮筋腫細胞의 增殖抑制 및 세포자멸사에 미치는 영향김동철, 백승희, 이화경대한한방부인과학회 · 대한한방부인과학회지2006 · 제19권 제3호p53-결핍 Jurkat T 임파구의 세포사멸에 대한 鷄血藤 물 추출물의 세포주기 억제효과김대근, 김원신, 김춘관, 박영준, 송호준, 이형철, 전병훈, 황상구한의병리학회 · 동의생리병리학회지2001 · 제15권 제6호자궁경부암세포에 대한 天花粉의 성장억제 및 세포사멸효과이현희, 임은미대한한방부인과학회 · 대한한방부인과학회지2005 · 제18권 제3호계혈등 EtOAc subfraction-2가 종양전이 억제효과에 관한 연구김성훈, 유시용, 이종수, 이현철, 허정은한의병리학회 · 동의생리병리학회지2003 · 제17권 제2호

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