Sweet BV 시술이 Rat의 중추신경계에 미치는 영향 - 기능관찰 종합평가를 이용하여-
Effects of Sweet Bee Venom on the Central Nervous System in Rats -using the Functional Observational Battery-
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Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results:1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.
Abstract
Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results:1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.
참고문헌 (32)
- Academic committee of KPI, "Pharmacopuncturology", pp.167~199 (2008), Elsevier Korea
- Kwon KR, "An Experimental Study with Bee Venom Therapy on Anti-inflammatory and Analgesic Effects", The Journal of Korean Acupuncture & Moxibustion Society, 15(2), pp.97~103 (1998)
- Ko HK, "Experimental Studies on the Effect of Bee Venom Theraphy on the Analgesic, Antipyretic and Anti-inflammatory Action", Korean Journal of Oriental Medicine, 13(1), pp.283~292 (1992)
- Kwon KR, "The Effect of Bee Venom Aqua-acupuncture on the Antitumor and Immune Response in the Epithelioma by 3 - MCA", The Journal of Korean Acupuncture & Moxibustion Society, 14(12), pp.151~172 (1997)
- 이성노, "蜂藥鍼治療의 退行性膝關節炎에 대한 臨床的 考察", 대한침구의학회지, 20(5), pp.6~ (2003), 대한침구의학회
- Wang WH, "Clincal Investigation Compared with the Effects of the Bee Venom - Acupuncture on Knee Joint with Osteoarthritis", The Journal of Korean Acupuncture & Moxibustion Society, 18(3), pp.35~47 (2001)
- Kim TH, "Three cases of affections of the hip treated with Korean Bee-Venom therapy", Journal of Korean Pharmacopuncture Institute, 4(3), pp.122~130 (2001)
- 이상훈, "봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향", 대한침구의학회지, 20(2), pp.7~ (2003), 대한침구의학회
- 이상훈, "무작위 대조 이중맹검 시험을 통한 봉독 약침의 류마티스 관절염 치료 효과 연구", 대한침구의학회지, 20(6), pp.9~ (2003), 대한침구의학회
- 김경운, "요추간판탈출증에 봉약침의 근위 취혈과 근위 및 원위 취혈의 비교", 대한침구의학회지, 22(6), pp.181~187 (2005), 대한침구의학회
- 이건목, "蜂藥鍼을 위주로 한 腰椎椎間板脫出症 의 突出型 患者(protrusion disc patients) 에 대한 臨床的 考察", 대한침구의학회지, 21(5), pp.13~26 (2004), 대한침구의학회
- 전형준, "봉약침으로 치료한 요추간판탈출증 환자의 임상적 평가", 대한침구의학회지, 20(5), pp.7~ (2003), 대한침구의학회
- 이대용, "中風後遺症으로 인한 肩臂痛 患者의 蜂藥鍼治療에 대한 臨床的 考察", 대한침구의학회지, 23(4), pp.69~80 (2006), 대한침구의학회
- 정경숙, "뇌졸중 후 중추성 통증 환자에 대한 봉독약침요법 치료 효과에 대한 임상적 연구", 대한침구의학회지, 22(3), pp.69~76 (2005), 대한침구의학회
- 최영권, "Gel filtration chromatography와 propionic acid", urea polyacrylamide gel electrophoresis를 이용한 봉독 성분의 분리, 2006, pp.9(2), 105~111[DOI]
- Barbara, "Chemistry and Pharmacology of Honey Bee venom", pp.329~402 (1986), Academic Press
- Park HJ, "Antiarthritic effect of Bee Venom", Arthritis & Rheumatism, 50(11), pp.3504~3515 (2004)
- Park HJ, "JNK pathway is involved on the inhibition of inflammatory target gene expression and NF-κB activation by melittin", J. Inflammation, 5(7), pp.1~13 (2008)
- 김영진, "Sweet BV의 rat를 이용한 단회 근육시술 독성시험", 대한약침학회지, 12(4), pp.5~32 (2009), 대한약침학회
- 식품의약품안전청, "식품의약품안전청고시 제2005-60호", ~
- Irwin, S, "Comprehensive behavioural assessment; A systematic quantitative procedure for assessing the behavioural and physiologic state of the mouse", Psychopharmacologia, 13, pp.222~257 (1968)
- 허인회, "독성학", pp.9~10 (1993), 신일상사
- 식품의약품안전청, "식품의약품안전청고시 제2008-56호", ~
- Redfern W.S., "Safety pharmacology-a progressive approach", Fundamental and Clinical Pharmacology, 16, pp.161~173 (2002)
- Moser V.C., "Comparison of chlordimeform and carbaryl using a functional observational battery", Fundam. Appl. Toxicol, 11, pp.189~206 (1988)
- Anon, "ICHS7A-Note for Guidance on Safety Pharmacology Studies for Human Pharmaceuticals" (2000), European Agency for the Evaluation of Medicinal Products, Committee for Proprietary Medicinal Products(CPMP)
- Hwang YJ, "Clinical Study of Anaphylaxis on Bee-Venom Acupuncture", The Journal of Korean Acupuncture & Moxibustion Society, 17(4), pp.149~159 (2000)
- 윤현민, "봉약침요법으로 발생한 Anaphylaxis에 대한 임상보고", 대한침구의학회지, 22(4), pp.179~188 (2005), 대한침구의학회
- 권기록, "Sweet BV의 함량분석과 시술 부위별 LD50관찰", 대한약침학회지, 10(2), pp.81~86 (2007), 대한약침학회
- 권기록, "Sweet BV의 성분분석과 항체역가 및 allergy 반응에 대한 임상적 연구", 대한약침학회지, 9(2), pp.79~86 (2006), 대한약침학회
- 이진선, "봉약침(Bee Venom)과 Sweet Bee Venom의 Allergy 반응에 대한 비교연구", 대한약침학회지, 9(3), pp.61~77 (2006), 대한약침학회
- Renata MS, "Structural and functional behavior of biologically active monomeric melittin", J. Molecular Graphics and Modelling, 25, pp.767~772 (2007)