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Sweet Bee Venom의 rat를 이용한 4주 반복 근육시술 독성시험

Study of four weeks repeated-dose toxic test of Sweet Bee Venom in rats

권기록|권혜연
대한약침학회 (Korean Pharmacopuncture Institute)|2011.03|Journal of Pharmacopuncture|제14권 제1호|pp. 5-24

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참고문헌

키워드

melittinSweet Bee Venomfour weeksrepeated dose toxicity
주제분류한의학

초록

Objectives: This study was performed to analyse four weeks repeated -dose toxicity of Sweet Bee Venom (SBV-pure melittin, the major component of honey bee venom) in rats. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP)at Biotoxtech Company, a non-clinical study authorized institution. Male and female rats of 5 weeks old were chosen for the pilot study of four weeks repeated-dose toxicity and was injected at the level of 0.56 ㎎/㎏ body weight (eighty times higher than the clinical application dosage as the high dosage), followed by 0.28 and 0.14 ㎎/㎏ as midium and low dosage, respectively. Equal amount of normal saline was injected as the control group every day for four weeks. Results:1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups appealed pain sense in the treating time compared to the control group, and side effects such as hyperemia and movement disorder were observed around the area of injection in all experiment groups, and the higher dosage in treatment, the higher occurrence in side effects. 3. Concerning weight measurement, neither male nor female groups showed significant changes compared to the control group. 4. Concerning to the CBC and biochemistry, all experiment groups didn't show any significant changes compared to the control group. 5. Concerning weight measurement of organs, experiment groups didn't show any significant changes compared to the control group. 6. To verify abnormalities of organs and tissues, those such as cerebellum, cerebrum, liver, lung, kidney,and spinal cords were removed and we conducted histologocal observation with H-E staining. Concerning the histologocal observation of liver tissues, some fatty changes were observed around portal vein in 0.56 ㎎/㎏ experiment group. But another organs were not detected in any abnormalities. 7. The proper high dosage of SBV for the thirteen weeks repeated test in rats may be 0.28 ㎎/㎏ in one time. Conclusion: Above findings suggest that SBV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.

Abstract

Objectives: This study was performed to analyse four weeks repeated -dose toxicity of Sweet Bee Venom (SBV-pure melittin, the major component of honey bee venom) in rats. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP)at Biotoxtech Company, a non-clinical study authorized institution. Male and female rats of 5 weeks old were chosen for the pilot study of four weeks repeated-dose toxicity and was injected at the level of 0.56 ㎎/㎏ body weight (eighty times higher than the clinical application dosage as the high dosage), followed by 0.28 and 0.14 ㎎/㎏ as midium and low dosage, respectively. Equal amount of normal saline was injected as the control group every day for four weeks. Results:1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups appealed pain sense in the treating time compared to the control group, and side effects such as hyperemia and movement disorder were observed around the area of injection in all experiment groups, and the higher dosage in treatment, the higher occurrence in side effects. 3. Concerning weight measurement, neither male nor female groups showed significant changes compared to the control group. 4. Concerning to the CBC and biochemistry, all experiment groups didn't show any significant changes compared to the control group. 5. Concerning weight measurement of organs, experiment groups didn't show any significant changes compared to the control group. 6. To verify abnormalities of organs and tissues, those such as cerebellum, cerebrum, liver, lung, kidney,and spinal cords were removed and we conducted histologocal observation with H-E staining. Concerning the histologocal observation of liver tissues, some fatty changes were observed around portal vein in 0.56 ㎎/㎏ experiment group. But another organs were not detected in any abnormalities. 7. The proper high dosage of SBV for the thirteen weeks repeated test in rats may be 0.28 ㎎/㎏ in one time. Conclusion: Above findings suggest that SBV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.

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관련 논문AI추천

Sweet BV의 rat를 이용한 단회 근육시술 독성시험권기록, 김영진, 임청산대한약침학회 · Journal of Pharmacopuncture2009 · 제12권 제4호Sweet Bee Venom의 비글견을 이용한 4주 반복 근육시술 독성시험권기록, 박재석, 이광호대한약침학회 · Journal of Pharmacopuncture2010 · 제13권 제4호Study on a 4-Week Recovery Test of Sweet Bee Venom after a 13-Week, Repeated, Intramuscular Dose Toxicity Test in Sprague-Dawley Rats강현민, 권기록, 김병우, 이광호, 이승배, 임청산대한약침학회 · Journal of Pharmacopuncture2014 · 제17권 제2호Study of a 13-weeks, Repeated, Intramuscular Dose, Toxicity Test of Sweet Bee Venom in Sprague-Dawley Rats강현민, 권기록, 이광호, 임청산대한약침학회 · Journal of Pharmacopuncture2014 · 제17권 제2호Sweet Bee Venom의 비글견을 이용한 단회근육시술 독성시험권기록, 윤휘철, 이광호대한약침학회 · Journal of Pharmacopuncture2010 · 제13권 제4호Intravenous Single Dose Toxicity of Sweet Bee Venom in Sprague-Dawley Rats권기록, 선승호, 유준상, 이광호대한약침학회 · Journal of Pharmacopuncture2015 · 제18권 제3호

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