Maternal Swimming Exercise During Pregnancy Improves Memory Through Enhancing Neurogenesis and Suppressing Apoptosis via Wnt/β-Catenin Pathway in Autistic Mice
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Purpose: Wnt pathway is closely related to neurodevelopmental process associated with cognitive function. After administration of valproic acid to the pregnant mice, the effect of swimming exercise of pregnant mice on the memory, neuronal production, and apoptosis of pups was studied in relation with Wnt/β-catenin signaling pathway. Methods: On day 12 of pregnancy, mice were injected subcutaneously with 400-mg/kg valproic acid. The pregnant mice in the control with swimming exercise group and in the valproic acid injection with swimming exercise group were allowed for swimming for 30 minutes one time per a day, repeated 5 days per a week, during 3 weeks. Step-through avoidance task and Morris water maze task for memory function, immunohistochemistry for 5-bromo-2’-deoxyuridine (BrdU)-positive cells and western blot for brain-derived neurotrophic factor (BDNF), Wnt, β-catenin, Bcl-2 related X protein (Bax), B-cell lymphoma 2 (Bcl-2), cleaved caspase-3 were carried out. Results: Maternal swimming exercise during pregnancy improved memory function, increased BDNF expression, and neuronal proliferation in the valproic acid injected pups. Maternal swimming exercise during pregnancy suppressed Wnt expression and phosphorylation of β-catenin in the valproic acid injected pups. Maternal swimming exercise inhibited Bax and cleaved caspase-3 expression and increased Bcl-2 expression in the valproic acid injected pups. Conclusions: Maternal swimming exercise during pregnancy improved memory function by increasing cell proliferation and inhibiting apoptosis through Wnt/β-catenin signaling cascade activation in the valproic acid injected pups. Maternal swimming exercise during pregnancy may have a protective effect on factors that induce autism in the fetus.
Abstract
Purpose: Wnt pathway is closely related to neurodevelopmental process associated with cognitive function. After administration of valproic acid to the pregnant mice, the effect of swimming exercise of pregnant mice on the memory, neuronal production, and apoptosis of pups was studied in relation with Wnt/β-catenin signaling pathway. Methods: On day 12 of pregnancy, mice were injected subcutaneously with 400-mg/kg valproic acid. The pregnant mice in the control with swimming exercise group and in the valproic acid injection with swimming exercise group were allowed for swimming for 30 minutes one time per a day, repeated 5 days per a week, during 3 weeks. Step-through avoidance task and Morris water maze task for memory function, immunohistochemistry for 5-bromo-2’-deoxyuridine (BrdU)-positive cells and western blot for brain-derived neurotrophic factor (BDNF), Wnt, β-catenin, Bcl-2 related X protein (Bax), B-cell lymphoma 2 (Bcl-2), cleaved caspase-3 were carried out. Results: Maternal swimming exercise during pregnancy improved memory function, increased BDNF expression, and neuronal proliferation in the valproic acid injected pups. Maternal swimming exercise during pregnancy suppressed Wnt expression and phosphorylation of β-catenin in the valproic acid injected pups. Maternal swimming exercise inhibited Bax and cleaved caspase-3 expression and increased Bcl-2 expression in the valproic acid injected pups. Conclusions: Maternal swimming exercise during pregnancy improved memory function by increasing cell proliferation and inhibiting apoptosis through Wnt/β-catenin signaling cascade activation in the valproic acid injected pups. Maternal swimming exercise during pregnancy may have a protective effect on factors that induce autism in the fetus.
참고문헌 (32)
- Mahoney AD, "Autism spectrum disorders and prematurity : a review across gestational age subgroups", Adv Neonatal Care, 13, pp.247~251 (2013)
- Esposito G, "An exploration of symmetry in early autism spectrum disorders : analysis of lying", Brain Dev, 31, pp.131~138 (2009)
- Vallée A, "Warburg effect hypothesis in autism spectrum disorders", Mol Brain, 11, pp.1~ (2018)
- Clevers H, "Wnt", β-catenin signaling and disease, 2012, pp.149, 1192~1205
- Huang J, "Maintenance of hematopoietic stem cells through regulation of Wnt and mTOR pathways", Nat Med, 18, pp.1778~1785 (2012)
- Chen J, "Dysregulation of the IGF-I", PI3K, mTOR signaling pathway in autism spectrum disorders, pp.Int J Dev Neurosci, 2014[DOI]
- Sun Q, "Mammalian target of rapamycin up-regulation of pyruvate kinase isoenzyme type M2 is critical for aerobic glycolysis and tumor growth", Proc Natl Acad Sci U S A, 108, pp.4129~4134 (2011)
- Lin Ru Wang, "Effects of treadmill exercise on the anxiety-like behavior through modulation of GSK3β", β-catenin signaling in the maternal separation rat pup, 2019, pp.15(2), 206~212[DOI]
- Quiroz JA, "Mitochondrially mediated plasticity in the pathophysiology and treatment of bipolar disorder", Neuropsychopharmacology, 33, pp.2551~2565 (2008)
- Krumm N, "A de novo convergence of autism genetics and molecular neuroscience", Trends Neurosci, 37, pp.95~105 (2014)
- Go HS, "Prenatal exposure to valproic acid increases the neural progenitor cell pool and induces macrocephaly in rat brain via a mechanism involving the GSK-3β", β-catenin pathway, 2012, pp.63, 1028~1041
- Voss MW, "Bridging animal and human models of exercise-induced brain plasticity", Trends Cogn Sci, 17, pp.525~544 (2013)
- Vivar C, "All about running: synaptic plasticity, growth factors and adult hippocampal neurogenesis", Curr Top Behav Neurosci, 15, pp.189~210 (2013)
- Cotman CW, "Exercise builds brain health : key roles of growth factor cascades and inflammation", Trends Neurosci, 30, pp.464~472 (2007)
- Duzel E, "Can physical exercise in old age improve memory and hippocampal function", Brain (2016)
- Bayod S, "Wnt pathway regulation by long-term moderate exercise in rat hippocampus", Brin Res, 1543, pp.38~48 (2014)
- Marcelino TB, "Effect of maternal exercise on biochemical parameters in rats submitted to neonatal hypoxia-ischemia", Brain Res, 1622, pp.91~101 (2015)
- Gomes da Silva S, "Maternal exercise during pregnancy increases BDNF levels and cell numbers in the hippocampal formation but not in the cerebral cortex of adult rat offspring", PLoS One, 11, pp.e0147200~ (2016)
- Wagner GC, "A new neurobehavioral model of autism in mice : pre-and postnatal exposure to sodium valproate", J Autism Dev Disorder, 36, pp.779~793 (2006)
- 박상서, "Effects of swimming exercise on social isolation-induced memory impairment and apoptosis in old rats", Journal of exercise rehabilitation, 16(3), pp.234~241 (2020), 한국운동재활학회[DOI]
- 김미아, "Berberine Ameliorates Brain Inflammation in Poloxamer 407-Induced Hyperlipidemic Rats", International Neurourology Journal, 23, pp.102~110 (2019), 대한배뇨장애요실금학회[DOI]
- 지은상, "Treadmill exercise in obese maternal rats during pregnancy improves short-term memory through neurogenesis in the hippocampus of rat pups", Journal of exercise rehabilitation, 16(5), pp.392~397 (2020), 한국운동재활학회[DOI]
- 박상서, "Treadmill exercise ameliorates nicotine withdrawal-induced symptoms", Journal of exercise rehabilitation, 15(3), pp.383~391 (2019), 한국운동재활학회[DOI]
- 고영준, "Treadmill exercise in obese maternal rats during pregnancy improves spatial memory through activation of phosphatidylinositol 3-kinase pathway in the hippocampus of rat pups", Journal of exercise rehabilitation, 16(6), pp.483~488 (2020), 한국운동재활학회[DOI]
- 이재민, "Wnt signaling pathway is implicated in the alleviating effect of treadmill exercise on maternal separation-induced depression", Journal of exercise rehabilitation, 15(2), pp.200~205 (2019), 한국운동재활학회[DOI]
- Bölte S, "The Gestalt of functioning in autism spectrum disorder: results of the international conference to develop final consensus International Classification of Functioning, Disability and Health core sets", Autism, 23, pp.449~467 (2019)
- Gobbi G, "Sodium-and magnesium-valproate in vivo modulate glutamatergic and GABAergic synapses in the medial prefrontal cortex", Psychopharmacology(Berl), 185, pp.255~262 (2006)
- Markram K, "The intense world theory-a unifying theory of the neurobiology of autism", Front Hum Neurosci, 4, pp.224~ (2010)
- Kataoka S, "Autism-like behaviours with transient histone hyperacetylation in mice treated prenatally with valproic acid", Int J Neuropsychopharmacol, 16, pp.91~103 (2013)
- Roullet FI, "Behavioral and molecular changes in the mouse in response to prenatal exposure to the anti-epileptic drug valproic acid", Neuroscience, 170, pp.514~522 (2010)
- Monti B, "Biochemical, molecular and epigenetic mechanisms of valproic acid neuroprotection", Curr Mol Pharmacol, 2, pp.95~109 (2009)
- 김상훈, "Treadmill exercise ameliorates impairment of spatial learning memory in pups born to old and obese mother rats", Journal of exercise rehabilitation, 17(4), pp.234~240 (2021), 한국운동재활학회[DOI]