Single Intramuscular-dose Toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) in Sprague-Dawley Rats
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Objectives: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices under a request by the Korean Pharmacopuncture Institute. This experiment was performed based on the testing standards of “Toxicity Test Standards for Drugs” by the Ministry of Food and Drug Safety. Subjects were divided into 4 groups: 1 control group in which normal saline was administered and 3 test groups in which 0.1, 0.5 or 1.0 mL of WCF was administered; a single intramuscular dose was injected into 5 males and 5 females in each group. General symptoms and body weights were observed/measured for 14 days after injection. At the end of the observation period, hematological and clinical chemistry tests were performed, followed by necropsy and histopathological examinations of the injected sections. Results: No mortalities were observed in any group. Also, symptoms, body weight, hematology, clinical chemistry and necropsy were not affected. However, histopathological examination of the injected part in one female in the 1.0-mL group showed infiltration of mononuclear cells and a multi-nucleated giant cell around eosinophilic material. Conclusion: Administration of single intramuscular doses of WCF in 3 groups of rats showed that the approximate lethal dose of WCF for all rats was in excess of 1.0 mL, as no mortalities were observed for injections up to and including 1.0 mL.
Abstract
Objectives: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices under a request by the Korean Pharmacopuncture Institute. This experiment was performed based on the testing standards of “Toxicity Test Standards for Drugs” by the Ministry of Food and Drug Safety. Subjects were divided into 4 groups: 1 control group in which normal saline was administered and 3 test groups in which 0.1, 0.5 or 1.0 mL of WCF was administered; a single intramuscular dose was injected into 5 males and 5 females in each group. General symptoms and body weights were observed/measured for 14 days after injection. At the end of the observation period, hematological and clinical chemistry tests were performed, followed by necropsy and histopathological examinations of the injected sections. Results: No mortalities were observed in any group. Also, symptoms, body weight, hematology, clinical chemistry and necropsy were not affected. However, histopathological examination of the injected part in one female in the 1.0-mL group showed infiltration of mononuclear cells and a multi-nucleated giant cell around eosinophilic material. Conclusion: Administration of single intramuscular doses of WCF in 3 groups of rats showed that the approximate lethal dose of WCF for all rats was in excess of 1.0 mL, as no mortalities were observed for injections up to and including 1.0 mL.
참고문헌 (17)
- Korean Pharmacopuncture Institute, "Pharmacopuncturology", pp.21~23 (2008), Elsevier Korea LLC
- Yook TH., "Effects on the local thermal change following herbal acupuncture on D. I. T. I", The Acupuncture, 17(3), pp.57~68 (2000)
- Kang KS., "Clinical studies on the cases of side effect of CF herbal acupuncture treatment", Pharmacopuncture, 4(2), pp.65~71 (2001)
- Lee HT., "Effect of Cathami Flos aqua-acupuncture on arthritis induced by injecting Freund’s complete adjuvant", The Journal of East-West Medicine, 23(3), pp.6~20 (1998)
- 성현제, "Rainbow Power 요법 시술이 퇴행성 슬관절염에 미치는 임상 효능연구", Korean Journal of Acupuncture, 20(4), pp.8~98 (2003), 경락경혈학회
- 서정철, "The Effect of CF Herbal Acupuncture by Oswestry Disability Index", Journal of Pharmacopuncture, 4(3), pp.15~20 (2001), 대한약침학회[DOI]
- 오치석, "中脘에 施術한 紅花藥鍼이 抗癌 및 免疫機能에 미치는 影響", 대한침구의학회지, 21(5), pp.205~218 (2004), 대한침구의학회
- Kim YT., "The effects of aqua-acupuncture with Hirudo nipponica Whitman and Carthamus tinctorius L. on the intravascular coagulation induced by endotoxin in rats", The Acupuncture, 14(1), pp.464~477 (1997)
- Kim Kyung-Woon, "A Case of Alopecia Areata Treated with Bee Venom and Carthami Flos Herbal Acupuncture", Journal of Pharmacopuncture, 7(2), pp.91~96 (2004), 대한약침학회[DOI]
- 양승인, "홍화 약침을 병행한 희발월경 환자 치험 2례", Journal of Pharmacopuncture, 8(3), pp.107~113 (2005), 대한약침학회
- 임사비나, "The study of irritation and toxicity of Carthami oil aquapuncture solution applied topically to the skin and the eye mucous membrane", Journal of Pharmacopuncture, 3(1), pp.53~63 (2000), 대한약침학회[DOI]
- 안창석, "The Study on Acute and Subacute Toxicity and Sarcoma-180 Anti-cancer Effects of Carthami Tinctor-Fructus Herbal-acupuncture(CF)", Journal of Pharmacopuncture, 5(1), pp.7~26 (2002), 대한약침학회[DOI]
- 최석우, "봉약침과 홍화약침으로 치료한 레이노 현상을 동반한 수근관증후군 환자 1례", Journal of Pharmacopuncture, 12(1), pp.103~108 (2009), 대한약침학회
- 김성욱, "紅花藥針이 局所腦血流量 및 平均血壓에 미치는 實驗的 影響", 대한침구의학회지, 21(2), pp.167~182 (2004), 대한침구의학회
- 우동수, "홍화 약침액의 Nitric Oxide에 대한 소거효과", Journal of Pharmacopuncture, 10(2), pp.25~30 (2007), 대한약침학회
- 이종영, "경락약침의 윤제(CF, JsD)에 의한 부작용 사례보고", Journal of Pharmacopuncture, 10(3), pp.137~142 (2007), 대한약침학회
- Kim BW., "The side effect of lubricants pharmacopuncture in rat tissues", 13(1), pp.87~92 (2010)