Loss of Caveolin 1 is Associated With the Expression of Aquaporin 1 and Bladder Dysfunction in Mice
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Purpose: It is suggested that caveolin and aquaporin might be closely associated with bladder signal activity. We investigatedthe effect of the deletion of caveolin 1, using caveolin 1 knockout mice, on the expression of aquaporin 1 in order to identifytheir relation in the urothelium of the urinary bladder. Methods: The cellular localization and expressions of aquaporin 1 and caveolin 1, in the wild type and caveolin 1 knockoutmice urinary bladder, were examined by Western blot and immunofluorescence techniques. Results: Aquaporin 1 and caveolin 1 were coexpressed in the arterioles, venules, and capillaries of the suburothelial layer inthe wild type controls. Aquaporin 1 protein expression was significantly higher in the caveolin 1 knockout mice than in thewild type controls (P<0.05). Conclusions: The results imply that aquaporin 1 and caveolin 1 may share a distinct relation with the bladder signal activity. This might play a specific role in bladder dysfunction.
Abstract
Purpose: It is suggested that caveolin and aquaporin might be closely associated with bladder signal activity. We investigatedthe effect of the deletion of caveolin 1, using caveolin 1 knockout mice, on the expression of aquaporin 1 in order to identifytheir relation in the urothelium of the urinary bladder. Methods: The cellular localization and expressions of aquaporin 1 and caveolin 1, in the wild type and caveolin 1 knockoutmice urinary bladder, were examined by Western blot and immunofluorescence techniques. Results: Aquaporin 1 and caveolin 1 were coexpressed in the arterioles, venules, and capillaries of the suburothelial layer inthe wild type controls. Aquaporin 1 protein expression was significantly higher in the caveolin 1 knockout mice than in thewild type controls (P<0.05). Conclusions: The results imply that aquaporin 1 and caveolin 1 may share a distinct relation with the bladder signal activity. This might play a specific role in bladder dysfunction.
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