초오 약침 개발을 위한 백서 독성 연구 및 생리활성에 대한 연구
The Study on toxicity and biological activities of Aconiti ciliare tuber Pharmacopuncture in Rats
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Objective : We investigate toxicity of Aconiti ciliare tuber and antioxidant activity of Aconiti ciliare tuber Pharmacopuncture to develop safe Aconiti ciliare tuber Pharmacopuncture and find out the effect. Methods : In order to investigate toxicity of Aconiti ciliare tuberm, we administered Aconiti ciliare tuberm orally to rats and examined the survival rate, comparing with the survival rate of rats administered by Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae. We examined the in vitro biological activity of Aconiti ciliare tuber Pharmacopuncture, including the total polyphenol content, and ABTS radical scavenging. Results and Conclusions :The LD50 of Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae was 9.0g/kg, on the other hand, the LD50 of Aconiti ciliare tuberm was more than 15g/kg. The total polyphenol contents of Aconiti ciliare tuberm Pharmacopuncture was 2.31mg/L. The 2,2'-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS) was 10.26%. We conclude that Aconiti ciliare tuberm is not highly toxic, Aconiti ciliare tuber Pharmacopuncture has a little antioxidant effect.
Abstract
Objective : We investigate toxicity of Aconiti ciliare tuber and antioxidant activity of Aconiti ciliare tuber Pharmacopuncture to develop safe Aconiti ciliare tuber Pharmacopuncture and find out the effect. Methods : In order to investigate toxicity of Aconiti ciliare tuberm, we administered Aconiti ciliare tuberm orally to rats and examined the survival rate, comparing with the survival rate of rats administered by Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae. We examined the in vitro biological activity of Aconiti ciliare tuber Pharmacopuncture, including the total polyphenol content, and ABTS radical scavenging. Results and Conclusions :The LD50 of Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae was 9.0g/kg, on the other hand, the LD50 of Aconiti ciliare tuberm was more than 15g/kg. The total polyphenol contents of Aconiti ciliare tuberm Pharmacopuncture was 2.31mg/L. The 2,2'-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS) was 10.26%. We conclude that Aconiti ciliare tuberm is not highly toxic, Aconiti ciliare tuber Pharmacopuncture has a little antioxidant effect.
참고문헌 (28)
- Shin MK, "Clinical Herbology", pp.498~499 (1986), Namsandang
- Kim CM, "The complete translation of Chinese herbal medicine", pp.2396~2404 (1998), Jungdam
- 최도일, "초오가 포함된 단방약에 의한 Aconitine 중독", 대한응급의학회지, 13(2), pp.175~180 (2002), 대한응급의학회
- 문정미, "초오 중독 환자에서 심혈관계의 변화에 대한 고찰", 대한응급의학회지, 13(1), pp.55~60 (2002), 대한응급의학회
- Lee GM, "Study of Aconiti tuber on normal Rats: change of body weight, transaminase", The Journal of Korea Acupuncture & Moxibustion Society, 16(1), pp.269~282 (1999)
- Yu CK, "The Bibilographic studies on Aconiti Ciliare Tuber and Radix Aconiti", The journal of pharmacopuncture, 4(2), pp.91~ (2001)
- Dewanto V, "Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity", J Agric Food Chem, 50, pp.3010~3014 (2002)
- Venden Berg R, "Applicability of an improved Trolox equivalent anti-oxidant capacity(TEAC) assay for evaluation of anti-oxidant capacity measurements of mixture", Food Chem, 66, pp.511~517 (1999)
- Rice-Evans CA, "Structure-antioxidant activity relationships of flavonoids and phenolic acids", Free Radic Biol Med, 20, pp.933~956 (1993)
- 김지은, "용매 종류와 효소 처리에 따른 쓴 메밀 추출물의 항산화 활성 및 α-Glucosidase 저해 활성의 변화", 한국식품영양과학회지, 38(8), pp.989~995 (2009), 한국식품영양과학회
- National College of Oriental Medicine Herbology, "Herbology", pp.331~332 (1995), Younglimsa
- Lee JH, "Effects on Gene Expression in the Rat Liver of Aconitine and Aconitum Species", Herbology, 22(2), pp.8~ (2007)
- Textbook Compilation Committee of Herbal pharmacology, "Herbal pharmacology", pp.430~436 (2009), Shinil
- Drug Botany Research, "Botanical drugs particulars", pp.178~179 (1984), Korean Learning Material Publishers
- Zhu GJ, "Radix aconitum, Sayucktang Analgesic, anti-inflammatory effect of pharmacodynamic study", Journal of Chinese medicine, 17(2), pp.104~106 (1992)
- Ju JP, "Radix aconitum, Digestive & Relieving pain pharmacology study", Journal of Chinese medicine, 17(4), pp.238~241 (1992)
- Yang GZ, "Warm & hot drug(Radix aconitum, Zingiberis recens rhizoma, Cinnamomi cortex) The effects of compound and a small compound on the sympathetic, adrenal gland, TSH, and LH", Journal of Chinese medicine, 7(11), pp.688~690 (1999)
- Juong SH, "The analgesic and antiimflamatory effect of A.I.P.R. aquq-acupuncture on the arthritis induced by Freund's complete Adjuvant in rats", The Journal of Korea Acupuncture & Moxibustion Society, 14(1), pp.334~336 (1997)
- Lee GM, "The Effects of Aqua-acupuncture with Radix aconiti on the Contraction of Rat`s Spinal Cord Injury", The Journal of Korea Acupuncture & Moxibustion Society, 16(1), pp.283~296 (1999)
- 정한성, "육계․부자의 약침과 경구투여가 2형 당뇨 모델 생쥐의 혈당강하에 미치는 영향", 대한침구의학회지, 27(5), pp.1~12 (2010), 대한침구의학회
- Jung Soo, "Chinese herbal medicine and control of adverse reactions", pp.430~436 (1992), Traditional Chinese Medicine Press
- Shin JY, "Explanation of Medicine", pp.208~209 (1991), Sungbosa
- The effect of Theragra chalcogramma, and soup simmered with Semen Glycine & Radix Glycyrrhizae on the toxity of Korean Aconiti ciliare tuber, "Kim Sung Nam", Sangi graduate school, 25~26
- Madsen HL, "Radical scavenging by dietary flavonoids", Eur Food Technol, 211, pp.240~246 (2000)
- Madsen HL, "Screen of antioxidative activity of spices", Food Chem, 57, pp.331~377 (1996)
- Kim HJ, "polyphenolic com-pound content and antioxidative activities by extract from seed, sprout and flower of safflower", J. Korean Soc. Food Sic. Nutr, 29, pp.1127~1132 (2000)
- Kyung Mi Yoo, "Evaluation of Different Methods of Antioxidant Measurement", Food Science and Biotechnology, 16(2), pp.177~182 (2007), 한국식품과학회
- NJ. Miller, "A novel method for measuring anti-oxidant capacity and its application to monitoring the antioxidant status in premature neonants", Clicical Science, 26, pp.265~277 (1993)
