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기니픽을 이용한 Sweet Bee Venom의 항원성 평가

Experimental study of antigenicity test of Sweet Bee Venom in Guinea Pigs

권기록|조병준
대한약침학회 (Korean Pharmacopuncture Institute)|2011.12|Journal of Pharmacopuncture|제14권 제4호|pp. 23-32

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참고문헌

키워드

PCAASAMelittinantigenicitySweet Bee Venom
주제분류한의학

초록

Objectives: This study was performed to examine the antigenic potential of pure melittin (Sweet Bee Venom - SBV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), authorized a non-clinical studies institution, under the regulations of Good Laboratory Practice (GLP). Antigenic potential of SBV was examined by active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) in guinea pigs. SBV was subcutaneously administered at 0.07 and 0.28㎎/㎏and also as a suspension with adjuvant (Freund's complete adjuvant: FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control response (5㎎/㎖- FCA). Results:1. In the ASA test, experimental groups showed some symptoms of anaphylaxis like piloerection,hyperpnea and staggering gait. 2. In the PCA test, low dosage group did not show any antibody responses, whereas high dosage group showed positive responses. 3. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. 4. In the autopsy of body, the abnormalities of lung were detected in the corpse. This means that the cause of death may induced anaphylactic shock. Conclusions: Above findings suggested that SBV had antigenic potential in guinea pig. Further studies on the subject should be conducted to yield more concrete evidences.

Abstract

Objectives: This study was performed to examine the antigenic potential of pure melittin (Sweet Bee Venom - SBV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), authorized a non-clinical studies institution, under the regulations of Good Laboratory Practice (GLP). Antigenic potential of SBV was examined by active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) in guinea pigs. SBV was subcutaneously administered at 0.07 and 0.28㎎/㎏and also as a suspension with adjuvant (Freund's complete adjuvant: FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control response (5㎎/㎖- FCA). Results:1. In the ASA test, experimental groups showed some symptoms of anaphylaxis like piloerection,hyperpnea and staggering gait. 2. In the PCA test, low dosage group did not show any antibody responses, whereas high dosage group showed positive responses. 3. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. 4. In the autopsy of body, the abnormalities of lung were detected in the corpse. This means that the cause of death may induced anaphylactic shock. Conclusions: Above findings suggested that SBV had antigenic potential in guinea pig. Further studies on the subject should be conducted to yield more concrete evidences.

참고문헌 (32)

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관련 논문AI추천

Sweet BV의 rat를 이용한 단회 근육시술 독성시험권기록, 김영진, 임청산대한약침학회 · Journal of Pharmacopuncture2009 · 제12권 제4호Sweet Bee Venom 시술이 Rat의 호흡기계에 미치는 영향권기록, 이종영대한약침학회 · Journal of Pharmacopuncture2011 · 제14권 제3호Sweet Bee Venom의 rat를 이용한 4주 반복 근육시술 독성시험권기록, 권혜연대한약침학회 · Journal of Pharmacopuncture2011 · 제14권 제1호Sweet Bee Venom의 비글견을 이용한 단회근육시술 독성시험권기록, 윤휘철, 이광호대한약침학회 · Journal of Pharmacopuncture2010 · 제13권 제4호Sweet Bee Venom의 비글견을 이용한 4주 반복 근육시술 독성시험권기록, 박재석, 이광호대한약침학회 · Journal of Pharmacopuncture2010 · 제13권 제4호Sweet BV 시술 후 발생한 과민반응 환자의 증례보고강계성, 권기록, 권혜연, 이광호, 임청산, 정희선대한약침학회 · Journal of Pharmacopuncture2009 · 제12권 제2호

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