Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention,little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here,we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.
Abstract
Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention,little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here,we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.
참고문헌 (16)
- Zhang D., "Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by ironmediated lipid peroxidation", Free Radic Biol Med, 20(1), pp.145~150 (1996)
- Yun TK., "Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds.", J Korean Med Sci., 16(7), pp.S6~18 (2001)
- Joo SS., "Reciprocal activity of ginsenosides in the production of proinflammatory repertoire, and their potential roles in neuroprotection in vitro", Planta Med, 71(5), pp.476~481 (2005)
- 권기록, "B16", F10세포를 이식한 C57BL, 대한침구의학회지, pp.2006, 23(1)[DOI]
- 장해영, "자연산 산삼, 산양삼 및 인삼의 항산화능 비교연구", Journal of Pharmacopuncture, 11(3), pp.67~78 (2008), 대한약침학회
- Lui JH., "The Ginsenosides of Various Ginseng Plants and Selected Products", J Nat Prod, 43(3), pp.340~346 (1980)
- Lim W., "Effects of population, age, and cultivation methods on ginsenoside content of wild American ginseng(Panax quinquefolium)", J Agric Food Chem, 53(22), pp.8498~8505 (2005)
- Shin SS., "Critic standardization and objectivity of mountain grown ginseng", Research Institute of Oriental Medicine Dong-Eui University, 5, pp.107~114 (2001)
- 권기록, "산삼과 장뇌삼 중 고려삼과 서양삼의 Pyrosequencing법에 의한 감별", 대한본초학회지, 19(4), pp.45~50 (2004), 대한본초학회
- 권기록, "Identification and Analysis of Differentially Expressed Genes in Mountain Cultivated Ginseng and Mountain Wild Ginseng", Journal of Acupuncture & Meridian Studies, 4(2), pp.123~128 (2011), 세계약침학회
- Debus RJ., "The manganese and calcium ions of photosynthetic oxygen evolution", Biochim Biophys Acta, 1102(3), pp.269~352 (1992)
- Hankamer B., "Boekema EJ. Structure and Membrane Organization of Photosystem Ii in Green Plants", Annu Rev Plant Physiol Plant Mol Biol, 48, pp.641~671 (1997)
- Nanba O., "Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559", Proc Natl Acad Sci USA, 84(1), pp.109~112 (1987)
- Bricker TM., "The structure and function of CP47 and CP43 in photosystem II", Photosynth Res, 72(2), pp.131~146 (2002)
- Diatchenko L., "Suppression subtractive hybridization : a method for generating differentially regulated or tissuespecific cDNA probes and libraries", Proc Natl Acad Sci USA, 93(12), pp.6025~6030 (1996)
- Diatchenko L., "Suppression subtractive hybridization : a versatile method for identifying differentially expressed genes", Methods Enzymol, 303, pp.349~380 (1999)