NFI-C 결손 생쥐의 유지와 특성
Maintenance and characteristics of the Nfic-deficient mouse
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The phylogenetically conserved nuclear factorI (NFI) gene family encodes site- specific transcription factors essential for the development of a number of organ systems, 까lere are four Nfi genes in mammals (j\뼈， Nfib, Nfic, and Njix) and singJe Nfi genes in Drosophila melanogaster, Caenorhabditis elegans, Anophelesspp‘, and other simple animals, NF1-C nul1 mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots while other tissueslorgans in the body, including ameloblasts appear to be unaffected and nOlmal, However, little is known about the mechanism of NF1 -C function in odontoblast differentiation and dentin formation, 1n 뻐s study, growth, fertility and morphological characteristics of Nfic-disruption were observed in order to maintain and characterize the NF1 -C nul\ mice, The results were as follows: 1. Fourteen heterozygotes corsses prodllced 215 Fl progeny distribllted: 134 wild type, 78 Nji-c.I . , and 4 Nfi-c.I . , The single PCR distinguishes both genotype and sex, Primer A and B amplified 545 bp of the Njic gene with neo-cassette, whereas primerò A and C amplified 3많 bp of the original Nfic gene 2, Gross observations of the mOllth and jaw of Nji-c-l- mice revealed virtually no mandibular incisors overgrown, thin maxillary mClsors 3. There were no growth retardation and increased moπ머ity of Nfi-c.l . mice 4, 1n morphological observation, abnormal odontoblasts of the NF1 -C nul1 mouse were round in shape, lost their p뼈rity， organized as a sheet of cel1s, and trapped in osteodentin-like mineralized tissue, These results suggested that this animal model provides invaluable information on lInderstanding the molecular processes responsible for odontoblast differentiation and dentin formation.
Abstract
The phylogenetically conserved nuclear factorI (NFI) gene family encodes site- specific transcription factors essential for the development of a number of organ systems, 까lere are four Nfi genes in mammals (j\뼈， Nfib, Nfic, and Njix) and singJe Nfi genes in Drosophila melanogaster, Caenorhabditis elegans, Anophelesspp‘, and other simple animals, NF1-C nul1 mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots while other tissueslorgans in the body, including ameloblasts appear to be unaffected and nOlmal, However, little is known about the mechanism of NF1 -C function in odontoblast differentiation and dentin formation, 1n 뻐s study, growth, fertility and morphological characteristics of Nfic-disruption were observed in order to maintain and characterize the NF1 -C nul\ mice, The results were as follows: 1. Fourteen heterozygotes corsses prodllced 215 Fl progeny distribllted: 134 wild type, 78 Nji-c.I . , and 4 Nfi-c.I . , The single PCR distinguishes both genotype and sex, Primer A and B amplified 545 bp of the Njic gene with neo-cassette, whereas primerò A and C amplified 3많 bp of the original Nfic gene 2, Gross observations of the mOllth and jaw of Nji-c-l- mice revealed virtually no mandibular incisors overgrown, thin maxillary mClsors 3. There were no growth retardation and increased moπ머ity of Nfi-c.l . mice 4, 1n morphological observation, abnormal odontoblasts of the NF1 -C nul1 mouse were round in shape, lost their p뼈rity， organized as a sheet of cel1s, and trapped in osteodentin-like mineralized tissue, These results suggested that this animal model provides invaluable information on lInderstanding the molecular processes responsible for odontoblast differentiation and dentin formation.