Intravenous Toxicity Study of Water-soluble Ginseng Pharmacopuncture in SD Rats
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초록
Objectives: Radix Ginseng has been used for thousandsof years to treat a wide variety of diseases. Radix ginsenghas also been used as a traditional medicine for boostingQi energy and tonifying the spleen and lungs. Traditionally,its effect could be obtained orally. Nowadays, anew method, the injection of herbal medicine, is beingused. This study was performed to investigate the single-dose intravenous toxicity of water-soluble ginsengpharmacopuncture (WSGP) in Sprague-Dawley (SD)rats. Methods: All experiments were carried out at Biotoxtech,an institute authorized to perform non-clinicalstudies under the regulation of Good Laboratory Practice(GLP). At the age of six weeks, 40 SD rats, 20 male ratsand 20 female rats, were allocated into one of 4 groupsaccording to the dosages they would receive. The WSGPwas prepared in the Korean Pharmacopuncture Instituteunder the regulation of Korea-Good ManufacturingPractice (K-GMP). Dosages of WSGP were 0.1, 0.5 and1.0 mL/animal for the experimental groups, and normalsaline was administered to the control group. The rat'sgeneral conditions and body weights, the results of their hematological and biochemistry tests, and their necropsyand histopathological findings were investigatedto identify the toxicological effect of WSGP injected intravenously. The effect was examined for 14 days afterthe WSGP injection. This study was performed underthe approval of the Institutional Animal Ethics Committeeof Biotoxtech. Results: No deaths were found in this single-dose toxicitytest on the intravenous injection of WSGP, and nosignificant changes in the rat's general conditions andbody weights, the results on their hematological andbiochemistry test, and their necropsy findings wereobserved during the test. The local area of the injectionsite showed minial change. The lethal dose wasassumed to be over 1.0 mL/animal in both sexes. Conclusion: These results indicate that WSGP is safeat dosages up to 1 mL/animal.
Abstract
Objectives: Radix Ginseng has been used for thousandsof years to treat a wide variety of diseases. Radix ginsenghas also been used as a traditional medicine for boostingQi energy and tonifying the spleen and lungs. Traditionally,its effect could be obtained orally. Nowadays, anew method, the injection of herbal medicine, is beingused. This study was performed to investigate the single-dose intravenous toxicity of water-soluble ginsengpharmacopuncture (WSGP) in Sprague-Dawley (SD)rats. Methods: All experiments were carried out at Biotoxtech,an institute authorized to perform non-clinicalstudies under the regulation of Good Laboratory Practice(GLP). At the age of six weeks, 40 SD rats, 20 male ratsand 20 female rats, were allocated into one of 4 groupsaccording to the dosages they would receive. The WSGPwas prepared in the Korean Pharmacopuncture Instituteunder the regulation of Korea-Good ManufacturingPractice (K-GMP). Dosages of WSGP were 0.1, 0.5 and1.0 mL/animal for the experimental groups, and normalsaline was administered to the control group. The rat'sgeneral conditions and body weights, the results of their hematological and biochemistry tests, and their necropsyand histopathological findings were investigatedto identify the toxicological effect of WSGP injected intravenously. The effect was examined for 14 days afterthe WSGP injection. This study was performed underthe approval of the Institutional Animal Ethics Committeeof Biotoxtech. Results: No deaths were found in this single-dose toxicitytest on the intravenous injection of WSGP, and nosignificant changes in the rat's general conditions andbody weights, the results on their hematological andbiochemistry test, and their necropsy findings wereobserved during the test. The local area of the injectionsite showed minial change. The lethal dose wasassumed to be over 1.0 mL/animal in both sexes. Conclusion: These results indicate that WSGP is safeat dosages up to 1 mL/animal.
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