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Immunomodulatory effects of silymarin after subacute exposure to mice: A tiered approach immunotoxicity screening

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초록

Silymarin is a flavonoid complex extracted from the Silybum marianum plant with a wide range of pharmacological and biochemical effects. In the present study, the immunomodulatory effects of silymarin were investigated in BALB/c mice. Silymarin was administered daily by intraperitoneal injection at doses of 50, 100 and 150mg/kg for 14 consecutive days. Following the exposure, host hematological parameters, spleen cellularity and histopathological examination, as well as delayed-type hypersensitivity (DTH) responses, hemagglutination titers (HA), splenocyte cytokine production and lymphocyte proliferation assay were studied in all of the test groups of animals. The results showed that the low dose of silymarin (50 mg/kg) could stimulate both cellular and humoral immune functions in the treated hosts. In addition, silymarin at 100 mg/kg appeared to impact on DTH responses and lymphoproliferation. Based on the finding here, it would seem that silymarin has efficient immunostimulant properties. As a recommendation, the application of silymarin along with acupuncture technique (herbal acupuncture) can be thought as a good plan to modulate and enhance the immune system for the management of several immunodeficiency disorders. However, further studies are required to demonstrate this hypothesis.

Abstract

Silymarin is a flavonoid complex extracted from the Silybum marianum plant with a wide range of pharmacological and biochemical effects. In the present study, the immunomodulatory effects of silymarin were investigated in BALB/c mice. Silymarin was administered daily by intraperitoneal injection at doses of 50, 100 and 150mg/kg for 14 consecutive days. Following the exposure, host hematological parameters, spleen cellularity and histopathological examination, as well as delayed-type hypersensitivity (DTH) responses, hemagglutination titers (HA), splenocyte cytokine production and lymphocyte proliferation assay were studied in all of the test groups of animals. The results showed that the low dose of silymarin (50 mg/kg) could stimulate both cellular and humoral immune functions in the treated hosts. In addition, silymarin at 100 mg/kg appeared to impact on DTH responses and lymphoproliferation. Based on the finding here, it would seem that silymarin has efficient immunostimulant properties. As a recommendation, the application of silymarin along with acupuncture technique (herbal acupuncture) can be thought as a good plan to modulate and enhance the immune system for the management of several immunodeficiency disorders. However, further studies are required to demonstrate this hypothesis.

참고문헌 (35)

  1. Farsam V, "Antitumor and immunomodu- latory properties of artemether and its ability to reduce CD4+ CD25+ FoxP3+ T reg cells in vivo", Int Immunop-harmacol, 11(11), pp.1802~1808 (2011)[DOI]
  2. Riahi-Zanjani B, "Safranal as a safe compound to mice immune system", Avicenna J Phytomed, 5(5), pp.441~ (2015)
  3. Shirani K, "Phytotrapy of cyclophospha-mide-induced immunosuppression", Environ Toxicol Pharmacol, 39(3), pp.1262~1275 (2015)[DOI]
  4. Noori S, "Tehranolide molecule modulates the immune response, reduce regulatory T cell and inhibits tumor growth in vivo", Molecul Immunol, 47(7), pp.1579~1584 (2010)[DOI]
  5. Rahnama M, "Evaluation of anti-cancer and immunomodulatory effects of carnosol in a Balb", c WEHI-164 fibrosarcoma model, 2015, pp.12(3), 231~238
  6. Hasani-Ranjbar S, "A systematic review of the potential herbal sources of future drugs effective in oxidant-related diseases", Infamma Allergy Drug Targets, 8(1), pp.2~10 (2009)[DOI]
  7. Elyasi S, "Topical Silymarin Administration for Prevention of Capecit-abine-Induced Hand-Foot Syndrome: A Randomized, Double‐Blinded, Placebo‐Controlled Clinical Trial", Phytother Res, 31(9), pp.1323~1329 (2017)[DOI]
  8. Elyasi S, "Effect of Oral Silymarin Administration on Prevention of Radiotherapy Induced Mucositis: A Randomized, Double‐Blinded, Placebo‐Controlled Clinical Trial", Phytother Res, 30(11), pp.1879~1885 (2016)[DOI]
  9. Karimi G, "Silymarin, a promising pharmacological agent for treatment of diseases", Iran J Basic Med Sci, 14, pp.308~ (2011)
  10. Agarwal R, "Anticancer potential of silymarin: from bench to bed side", Anticancer Res, 26(6B), pp.4457~4498 (2006)
  11. Vargas-Mendoza N, "Hepatoprotective effect of silymarin", World J Hepatol, 6(3), pp.144~ (2014)[DOI]
  12. Razavi BM, "Protective effect of silymarin against chemical-induced cardiotoxicity", Iran J Basic Med Sci., 19(9), pp.916~ (2016)
  13. Darvishi Khezri H, "Potential effects of silymarin and its flavonolignan components in patients with ‐-Talassemia major: a comprehensive review in 2015", Adv Pharmacol Sci, 2016 (2016)
  14. Gharagozloo M, "Silymarin suppress CD4+ T cell activation and proliferation: Effects on NF-<TEX>${\kappa}B$<", TEX> activity and IL-2 production, 2010, pp.61(5), 405~409
  15. Morishima C, "Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infection", Gastroenterol, 138(2), pp.671~681.e2 (2010)[DOI]
  16. Johnson VJ, "Physiological Responses of a Natural Antioxidant Flavonoid Mixture, Silymarin, in BALB", c Mice, 2003, pp.69(01), 44~49
  17. Lee JS, "Silibinin polarizes T1", T2 immune responses through the inhibition of immunostimulatory function of dendritic cells, 2007, pp.210(2), 385~397
  18. Vaid M, "Silymarin inhibits ultraviolet radiation-induced immune suppression through DNA repair-dependent activation of dendritic cells and stimulation of effector T cells", Biochem Pharmacol, 85(8), pp.1066~1076 (2013)[DOI]
  19. Mady FM, "Formulation and clinical evaluation of silymarin pluronic-lecithin organogels for treatment of atopic dermatitis", Drug Des Devel Ter, 10, pp.1101~ (2016)
  20. Choi YH, "Silibinin attenuates allergic airway inflammation in mice", Biochem Biophys Res Commun, 427(3), pp.450~455 (2012)[DOI]
  21. Bakhshaee M, "Effect of silymarin in the treatment of allergic rhinitis", Otolaryngol Head Neck Surg, 145(6), pp.904~909 (2011)[DOI]
  22. Luster MI, "Consensus work-shop on methods to evaluate developmental immunotoxicity", Environ health perspect, 111(4), pp.579~ (2003)
  23. Riahi B, "Immunotoxicity of paraquat after subacute exposure to mice", Food Chem Toxicol, 48(6), pp.1627~1631 (2010)[DOI]
  24. Neishabouri EZ, "Evaluation of immunotoxicity induced by diazinon in C57bl", 6 mice, 2004, pp.196(3), 173~179
  25. Sena LA, "Mitochondria are required for antigen-specific T cell activation through reactive oxygen species signaling", Immunity, 38(2), pp.225~236 (2013)[DOI]
  26. Schieke SM, "Coordination of mitochondrial bioenergetics with G1 phase cell cycle progression", Cell Cycle, 7(12), pp.1782~1787 (2008)[DOI]
  27. Byun HO, "Mitochondrial dysfunction by complex II inhibition delays overall cell cycle progression via reactive oxygen species production", J Cell Biochem, 104(5), pp.1747~1759 (2008)[DOI]
  28. Keramati MR, "Biochemical and hematological findings of Khorasan veterans 23 years after sulfur mustard exposure", J Res Med Sci, 18(10), pp.855~ (2013)
  29. Devadas S, "Discrete generation of superoxide and hydrogen peroxide by T cell receptor stimulation", J Exp Med, 195(1), pp.59~70 (2002)[DOI]
  30. Caroleo M, "Chronic administration of paraquat produces immunosuppression of T lymphocytes and astrocytosis in rats", Toxic Subst Mech, 15(3), pp.183~194 (1996)
  31. Annunziata M, "The levels of glutathione and hemoglobin in sheep erythrocytes as a function of age", Ital J Anim Sci, 3(3), pp.283~286 (2004)[DOI]
  32. Witko-Sarsat V, "Immunomodulatory role of phagocyte-derived chloramines involving lymphocyte glutathione", Mediators Inflamm, 2(3), pp.235~241 (1993)[DOI]
  33. Victor, V. M, "Immune cells: free radicals and antioxidants in sepsis", Int Immunopharmacol, 4(3), pp.327~347 (2004)[DOI]
  34. Grifths HR, "ROS as signalling molecules in T cells--evidence for abnormal redox signalling in the autoimmune disease, rheumatoid arthritis", Redox Rep, 10(6), pp.273~280 (2005)[DOI]
  35. Mahmoudi M, "Immunotoxicity induced in mice by subacute exposure to berberine", Immunotoxicol, 13(2), pp.255~262 (2016)[DOI]

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