Single-Dose Toxicity Study of Intramuscular Neuralgia-Pharmacopuncture Injection in Rats
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: Neuralgia-pharmacopuncture (NP) was recently developed as a water-soluble type of pharmacopuncture inspired by CS (care special pain)-pharmacopuncture. I aimed to evaluate the toxic response and approximate lethal dose of when NP when administered intramuscularly to Sprague Dawley rats. Methods: The experimental group was divided into the NP test substance group and the saline control group and administered at a dose of 1.0 mL/animal to the posterior thigh muscles on both sides using a 1 mL syringe; each group consisted of five males and five females. Each rat was monitored for clinical signs and changes in body weight for 14 days after a single intramuscular injection. After completing observation, necropsy findings and localized tolerance at the injection site were assessed via gross necropsy and histopathological examination. Results: No deaths occurred in the NP or control group, regardless of sex. During the observation period, no changes (such as general symptoms, weight change, or visual observation results at the time of autopsy) were judged to be due to the test substance. Histopathological examination showed no changes at the administration site judged to be caused by the test substance in either the male or female test substance administration groups. In addition, mononuclear cell infiltration of the outer membrane of the femoris muscle at the administration site was observed at the same frequency and extent in the control and NP groups, and was judged to be caused by physical stimulation by the injection needle; therefore, it had no toxicological significance. Conclusion: Based on the above results, the approximate lethal dose for a single intramuscular administration of the test substance NP in Sprague-Dawley rats was judged to be > 1.0 mL/animal, and there were no findings that were judged to be due to the test substance at the administration site.
Abstract
Objectives: Neuralgia-pharmacopuncture (NP) was recently developed as a water-soluble type of pharmacopuncture inspired by CS (care special pain)-pharmacopuncture. I aimed to evaluate the toxic response and approximate lethal dose of when NP when administered intramuscularly to Sprague Dawley rats. Methods: The experimental group was divided into the NP test substance group and the saline control group and administered at a dose of 1.0 mL/animal to the posterior thigh muscles on both sides using a 1 mL syringe; each group consisted of five males and five females. Each rat was monitored for clinical signs and changes in body weight for 14 days after a single intramuscular injection. After completing observation, necropsy findings and localized tolerance at the injection site were assessed via gross necropsy and histopathological examination. Results: No deaths occurred in the NP or control group, regardless of sex. During the observation period, no changes (such as general symptoms, weight change, or visual observation results at the time of autopsy) were judged to be due to the test substance. Histopathological examination showed no changes at the administration site judged to be caused by the test substance in either the male or female test substance administration groups. In addition, mononuclear cell infiltration of the outer membrane of the femoris muscle at the administration site was observed at the same frequency and extent in the control and NP groups, and was judged to be caused by physical stimulation by the injection needle; therefore, it had no toxicological significance. Conclusion: Based on the above results, the approximate lethal dose for a single intramuscular administration of the test substance NP in Sprague-Dawley rats was judged to be > 1.0 mL/animal, and there were no findings that were judged to be due to the test substance at the administration site.
참고문헌 (23)
- Jung C, "Pharmacopuncture clinical guide", pp.326~ (2023), Koonja publishing co
- Jeong Jin-Ho, "A Study on the Significance of Acupuncture and Pharmacopuncture Therapy for Cold Accumulation Through a Literature Review on the Historical Development Process in Cold Accumulation Treatment", Journal of Acupuncture Research, 39(4), pp.267~274 (2022), 대한침구의학회[DOI]
- Park JE, "Usage status and satisfaction with pharmacopuncture in Korea: a survey among Korean medicine doctors", Eur J Integr Med, 27, pp.121~130 (2019)
- Hwang Ji Hye, "Single-Dose Intramuscular Toxicity Study of SU-Eohyeol Pharmacopuncture in Rats", Journal of Pharmacopuncture, 25(3), pp.268~275 (2022), 대한약침학회[DOI]
- "Good laboratory practice (GLP)" (2017)[URL]
- "Partial amendment official announcement about toxicity test for drug" (2015)[URL]
- Lee MY, "Genotoxicity evaluation of Guibi-Tang extract using an in vitro bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus test", BMC Complement Altern Med, 14, pp.215~ (2014)
- Park JE, "A survey on Korean medicine doctor‘s use of Herbal Acupuncture and recognition of its national health insurance coverage", master, pp.2018
- Hwang Ji Hye, "Single-Dose Intramuscular Toxicity Test Using No-Pain Pharmacopuncture in Sprague–Dawley Rats", Journal of Pharmacopuncture, 26(1), pp.86~93 (2023), 대한약침학회[DOI]
- The co-textbook publishing committee of Korean medicine college, "Herbology" (2004), Younglimsa
- Subedi L, "Neuroprotective and anti-inflammatory activities of allyl isothiocyanate through attenuation of JNK", NF-κB, Int J Mol Sci, pp.2017, 18(7)[DOI]
- Jo AR, "Inhibitory effect of moschamine isolated from Carthamus tinctorius on LPS-induced inflammatory mediators via AP-1 and STAT1", 3 inactivation in RAW 264.7 macrophages, 2017, pp.27(23), 5245~5251
- Alwan AM, "Onco-immunity and therapeutic application of amygdalin : a review", J Oral Biol Craniofac Res, 13(2), pp.155~163 (2023)
- 황지혜, "요추 추간판탈출증 환자에 대한 봉약침과 CS약침을 병행한 한의학적 복합치료 효과에 대한 증례보고", Korean Journal of Acupuncture, 35(4), pp.239~246 (2018), 경락경혈학회[DOI]
- Jung C, "A clinical study of immune pharmacopuncturology" (2011), Kyungrak Medical Publishing co
- Gong HM, "A case report of herpes zoster patient treated with additional CS, V pharmacopuncture", J Korea Immuno-Yakchim Soc, 5(1), pp.27~36 (2016)
- Kim SK, "Single intramuscular dose toxicity study of CSII-YAKCHIM in Sprague-Dawley rats", J Korea Immuno-Yakchim Soc, 4(1), pp.1~7 (2015)
- Lee KJ, "Bacterial reverse mutation test of CSII-YAKCHIM", J Korea Immuno-Yakchim Soc, 4(1), pp.9~17 (2015)
- Lee MS, "In vivo micronucleus test of CSII-YAKCHIM in mice", J Korea Immuno-Yakchim Soc, 4(1), pp.19~24 (2015)
- Yang JW, "In vitro mammalian chromosomal aberration test of CSII-YAKCHIM", J Korea Immuno-Yakchim Soc, 4(1), pp.25~31 (2015)
- 정다정, "Single Intravenous-dose Toxicity of Water-soluble Carthami-flos Pharmacopuncture (WCF) in Rats", Journal of Pharmacopuncture, 17(3), pp.31~39 (2014), 대한약침학회[DOI]
- Jung C, "A pharmacokinetic study of HOpharmacopuncture solution", J Korea Immuno-Yakchim Soc, 6(1), pp.21~28 (2017)
- Kim HG, "A pharmacokinetic study of pharmacopuncture wild ginseng pharmacopuncture solution", J Korea Immuno-Yakchim Soc, 1(1), pp.63~79 (2012)