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皂角刺가 estradiol valerate로 유발된 白鼠의 다낭성 난소에 미치는 영향

Effects of Gleditsiae Spina(GS) on the Polycystic Ovary Induced by Estradiol Valerate in Rats

조성희|구희준
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2010.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제23권 제2호|pp. 71-84

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참고문헌

키워드

Polycystic ovary syndrome(PCOS)Glenditsia spinaAnti-hyperplasia drug
주제분류한의학

초록

Purpose: In the theory of traditional medicine, Glenditsia spina(GS) can resolve carbuncle, relive swelling, dispel wind and destroy parasites. This study was designed to investigate the effects of GS on gene expression of ovarian tissue in polycystic ovary syndrome(PCOS) rats. Methods: In this experiment, female rats injected with a single dose of 2 mg estradiol valerate(EV) and GS was given for 5 weeks. The genetic profile for the effects on ovarian tissue in PCOS rats was measured using microarray technique, and the functional analysis on these genes was conducted. Results: 985 genes were increased in control and restored to normal level in GS group. (B), 733 genes were decreased in control group and restored to normal level in GS group. (F). Metabolic pathways related in B group genes were Graft- versus-host disease, Allograft rejection, Autoimmune thyroid disease, Cytokine -cytokine receptor interaction, Small cell lung cancer, Type I diabetes mellitus. Metabolic pathways related in F group genes were Antigen processing and present, Adipocytokine signalling pathway, Focal adhesion, ECM-receptor interaction, Pancreatic cancer, Notch signalling pathway, Tight junction. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as c-Fos, c-Myc, ABL1 related in B group, MAPK8, RASA1, CALR related in F group that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conclusion: These results suggest possibility of GS as anti-cancer and anti- hyperplasia drug in PCOS. In addition, the present author also suggests that related mechanisms are involved in suppression of proto-oncogene such as c-Fos, c-Myc and ABL1, and in regulation of cell cycle such as RASA1.

Abstract

Purpose: In the theory of traditional medicine, Glenditsia spina(GS) can resolve carbuncle, relive swelling, dispel wind and destroy parasites. This study was designed to investigate the effects of GS on gene expression of ovarian tissue in polycystic ovary syndrome(PCOS) rats. Methods: In this experiment, female rats injected with a single dose of 2 mg estradiol valerate(EV) and GS was given for 5 weeks. The genetic profile for the effects on ovarian tissue in PCOS rats was measured using microarray technique, and the functional analysis on these genes was conducted. Results: 985 genes were increased in control and restored to normal level in GS group. (B), 733 genes were decreased in control group and restored to normal level in GS group. (F). Metabolic pathways related in B group genes were Graft- versus-host disease, Allograft rejection, Autoimmune thyroid disease, Cytokine -cytokine receptor interaction, Small cell lung cancer, Type I diabetes mellitus. Metabolic pathways related in F group genes were Antigen processing and present, Adipocytokine signalling pathway, Focal adhesion, ECM-receptor interaction, Pancreatic cancer, Notch signalling pathway, Tight junction. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as c-Fos, c-Myc, ABL1 related in B group, MAPK8, RASA1, CALR related in F group that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conclusion: These results suggest possibility of GS as anti-cancer and anti- hyperplasia drug in PCOS. In addition, the present author also suggests that related mechanisms are involved in suppression of proto-oncogene such as c-Fos, c-Myc and ABL1, and in regulation of cell cycle such as RASA1.

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皂角刺가 Estradiol valerate에 의하여 유발된 다낭성난소에 미치는 영향김형우, 신동선, 안원근, 조수인한의병리학회 · 동의생리병리학회지2011 · 제25권 제4호當歸가 다낭성난소증후군이 유발된 흰쥐 난소조직의 유전자 발현에 미치는 영향류기준, 조성희대한한방부인과학회 · 대한한방부인과학회지2011 · 제24권 제3호開鬱二陳湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소 발달에 미치는 영향김형준, 서일복, 윤문희, 이동녕대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제2호향부자가 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 영향김형우, 박경미, 양동선, 양승정, 조성희, 조수인대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제4호五積散이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF발현에 미치는 영향김종대, 김형준, 서일복, 이동녕대한한방부인과학회 · 대한한방부인과학회지2011 · 제24권 제1호調經種玉湯이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF 발현에 미치는 影響김형준, 서일복, 양준모, 이동녕대한한방부인과학회 · 대한한방부인과학회지2011 · 제24권 제2호

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