Antidiabetic Effect of Standardized Chrysanthemum rubellum Hydroethanolic Extract by Targeting α-Glucosidase and the PTP-1B Signaling Pathway for Alleviating Diabetes in Experimental Model
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Objectives: The study’s goal was to find out whether Chrysanthemum rubellum extract has anti-diabetic properties by concentrating on α-glucosidase and the PTP-1B signaling pathway. C. rubellum flowers were used for extraction using Methanol/water (80/20) as solvent. Methods: LC-MS techniques was used to check the presence of phytoconstituents present in C. rubellum extract. In vitro antidiabetic activity was evaluated using α-glucosidase inhibitory activity and PTP-1B signaling pathway. On Streptozotocin (STZ)-induced rats with diabetes, the in vivo antidiabetic efficacy was assessed using a test for oral glucose tolerance. Results: The phytoconstituents identified in the extract of C. rubellum were apigenin, diosmin, myricetin, luteolin, luteolin-7-glucoside, and Quercitrin as compound 1-6, respectively. Results showed that diosmin exhibited highest α-glucosidase inhibitory activity i.e. 90.39%. The protein level of PTP-1B was lowered and the insulin signalling activity was directly increased by compounds 1-6. The maximum blood glucose levels were seen in all groups’ OGTT findings at 30 minutes following glucose delivery, followed by gradual drops. In comparison to the control group, the extract’s glucose levels were 141 mg/dL at 30 minutes before falling to 104 mg/dL after 120 minutes. The current study has demonstrated, in summary, that extract with phytoconstituents reduce blood sugar levels in rats. Conclusion: This finding suggests that extract may reduce the chance of insulin resistance and shield against disorders like hyperglycemia.
Abstract
Objectives: The study’s goal was to find out whether Chrysanthemum rubellum extract has anti-diabetic properties by concentrating on α-glucosidase and the PTP-1B signaling pathway. C. rubellum flowers were used for extraction using Methanol/water (80/20) as solvent. Methods: LC-MS techniques was used to check the presence of phytoconstituents present in C. rubellum extract. In vitro antidiabetic activity was evaluated using α-glucosidase inhibitory activity and PTP-1B signaling pathway. On Streptozotocin (STZ)-induced rats with diabetes, the in vivo antidiabetic efficacy was assessed using a test for oral glucose tolerance. Results: The phytoconstituents identified in the extract of C. rubellum were apigenin, diosmin, myricetin, luteolin, luteolin-7-glucoside, and Quercitrin as compound 1-6, respectively. Results showed that diosmin exhibited highest α-glucosidase inhibitory activity i.e. 90.39%. The protein level of PTP-1B was lowered and the insulin signalling activity was directly increased by compounds 1-6. The maximum blood glucose levels were seen in all groups’ OGTT findings at 30 minutes following glucose delivery, followed by gradual drops. In comparison to the control group, the extract’s glucose levels were 141 mg/dL at 30 minutes before falling to 104 mg/dL after 120 minutes. The current study has demonstrated, in summary, that extract with phytoconstituents reduce blood sugar levels in rats. Conclusion: This finding suggests that extract may reduce the chance of insulin resistance and shield against disorders like hyperglycemia.
참고문헌 (18)
- Cortes-Justo E, "The function of the endocannabinoid system in the pancreatic islet and its implications on metabolic syndrome and diabetes", Islets, 15(1), pp.1~11 (2023)
- Fujiwara Y, "Relationship between diet", exercise and pharmacotherapy to enhance the GLP-1 levels in type 2 diabetes, 2019, pp.2(3), e00068~
- Ganesan K, "StatPearls" (2023), StatPearls Publishing
- Padhi S, "Type II diabetes mellitus : a review on recent drug based therapeutics", Biomed Pharmacother, 131, pp.110708~ (2020)
- Shinoyama H, "Floriculture, ornamental and plant biotechnology:advances and topical issues (Vol. II)", pp.140~163 (2006), Global Science Books
- 김양지, "Hypoglycemic effect of standardized Chrysanthemum zawadskii ethanol extract in high-fat diet", streptozotocin-induced diabetic mice and rats, 2018, pp.27(6), 1771~1779[DOI]
- Shao Y, "Chrysanthemum indicum L.: a comprehensive review of its botany, phytochemistry and pharmacology", Am J Chin Med, 48(4), pp.871~897 (2020)
- Liang WL, "The composition of chrysanthemum extracts and their pharmacological functions", STEMedicine, 2(5), pp.e69~ (2021)
- Akmal M, "Stat-Pearls" (2023), StatPearls Publishing
- Yamakage H, "Association of protein tyrosine phosphatase 1B gene polymorphism with the effects of weight reduction therapy on bodyweight and glycolipid profiles in obese patients", J Diabetes Investig, 12(8), pp.1462~1470 (2021)
- Sakaguchi M, "Phosphatase protector alpha4(α4)is involved in adipocyte maintenance and mitochondrial homeostasis through regulation of insulin signaling", Nat Commun, 13(1), pp.6092~ (2022)
- Zhang X, "A novel protein tyrosine phosphatase 1B inhibitor with therapeutic potential for insulin resistance", Br J Pharmacol, 173(12), pp.1939~1949 (2016)
- OECD, "Test No. 425: acute oral toxicity: up-and-down procedure" (2022), OECD
- Barik R, "Antidiabetic activity of aqueous root extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats", Indian J Pharmacol, 40(1), pp.19~22 (2008)
- Gershell L, "Type 2 diabetes market", Nat Rev Drug Discov, 4(5), pp.367~368 (2005)
- Koren S, "Inhibition of the protein tyrosine phosphatase PTP1B : potential therapy for obesity, insulin resistance and type-2 diabetes mellitus", Best Pract Res Clin Endocrinol Metab, 21(4), pp.621~640 (2007)
- Derosa G, "α-Glucosidase inhibitors and their use in clinical practice", Arch Med Sci, 8(5), pp.899~906 (2012)
- 국은비, "Anti-Alzheimer’s disease activity of compounds from the root bark of Morus alba L.", Archives of Pharmacal Research, 40(3), pp.338~349 (2017), 대한약학회