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紫河車 藥鍼이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향

Effects of Hominis Placenta Pharmacopuncture Therapy on the Experimentally-induced Endometriosis in the Rats

김형준|이동녕|유영기|신미란
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2014.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제27권 제1호|pp. 101-119|DOI http://dx.doi.org/10.15204/jkobgy2014.27.1.101

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키워드

VEGFCOX-2EndometriosisHominis Placenta PharmacopunctureMCP-1 TNF-α
주제분류한의학

초록

Objectives: This study was performed to investigate the effects of Hominis Placentapharmacopuncture (HPP) therapy on the experimentally-induced endometriosis in the rats. Materials and Methods: Endometriosis was induced in rats by autotransplantinguterine tissue to the peritoneum and divided them into three groups: (1) sham-operatedgroup (n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and HPP treated group. Sham-operatedgroup and control group were inject with normal saline once a every other day for30days, while treated group was injected with HPP extract once a every other dayfor same duration. Injected point of HPP and normal saline were subcutaneous tissueat Gwanwon (CV4) acupoint. Then we measured the body weight, the volume ofendometriotic implants, the weigh of uterus and ovaries, and investigated theconcentration of cytokines (MCP-1, TNF-α) in peritoneal fluids. Histopathology,immunohistochemisty for COX-2 and VEGF, and histochemistry for mast cell intransplanted uterine tissue were performed. Results: The volume (mm2) of endometriotic implants in HPP treated group(55.4±41.6) was significantly decreased (p<0.01) compared with control group (140±66.1). And the concentration (pg/ml) of MCP-1 in peritoneal fluids in HPP treated group(1117.6±60.5) was significantly decreased (p<0.01) compared with control group(1446.2±280.3). The concentration (pg/ml) of TNF-α in peritoneal fluids in HPP treatedgroup (80.6±31.4) was decreased (p<0.01) compared with control group (145.3±86.9). Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatorycell and angiogenesis in transplanted uterine tissue of HPP treated group wereweakly observed than those of control group. The COX-2 expression in endometrial,epithelial and stromal cells in transplanted uterine tissue of HPP treated group wasdecreased compared with control group. The VEGF expression of endometriotic epithelia,neovascular endothelia and stromal cell in transplanted uterine tissue of HPP treatedgroup were weakly observed than those of control goup. Conclusions: HPP is effect on Endometriosis of rats by Experimentally-induced.

Abstract

Objectives: This study was performed to investigate the effects of Hominis Placentapharmacopuncture (HPP) therapy on the experimentally-induced endometriosis in the rats. Materials and Methods: Endometriosis was induced in rats by autotransplantinguterine tissue to the peritoneum and divided them into three groups: (1) sham-operatedgroup (n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and HPP treated group. Sham-operatedgroup and control group were inject with normal saline once a every other day for30days, while treated group was injected with HPP extract once a every other dayfor same duration. Injected point of HPP and normal saline were subcutaneous tissueat Gwanwon (CV4) acupoint. Then we measured the body weight, the volume ofendometriotic implants, the weigh of uterus and ovaries, and investigated theconcentration of cytokines (MCP-1, TNF-α) in peritoneal fluids. Histopathology,immunohistochemisty for COX-2 and VEGF, and histochemistry for mast cell intransplanted uterine tissue were performed. Results: The volume (mm2) of endometriotic implants in HPP treated group(55.4±41.6) was significantly decreased (p<0.01) compared with control group (140±66.1). And the concentration (pg/ml) of MCP-1 in peritoneal fluids in HPP treated group(1117.6±60.5) was significantly decreased (p<0.01) compared with control group(1446.2±280.3). The concentration (pg/ml) of TNF-α in peritoneal fluids in HPP treatedgroup (80.6±31.4) was decreased (p<0.01) compared with control group (145.3±86.9). Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatorycell and angiogenesis in transplanted uterine tissue of HPP treated group wereweakly observed than those of control group. The COX-2 expression in endometrial,epithelial and stromal cells in transplanted uterine tissue of HPP treated group wasdecreased compared with control group. The VEGF expression of endometriotic epithelia,neovascular endothelia and stromal cell in transplanted uterine tissue of HPP treatedgroup were weakly observed than those of control goup. Conclusions: HPP is effect on Endometriosis of rats by Experimentally-induced.

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