LPS로 활성화된 대식세포에서 玄附理經湯의 시험관내 항염효과 및 Piroxicam과 병용효과
In Vitro Anti-inflammatory Effects of Hyunbulikyung-tang Aqueous Extracts on LPS activated Raw 264.7 Cells, and Synergic Effects with Piroxicam
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Objectives: To observe the in vitro anti-inflammatory effects of Hyunbulikyung-tangaqueous extracts (HBLKT) and the possible synergic combination effects with anonsteroidal anti-inflammatory drug, piroxicam. Methods: Anti-inflammatory effects of HBLKT (yield=16.17%) were observedon LPS activated raw 264.7 cells based on ED50 to cell viability, NO, PGE2, TNF-α,IL-1β and IL-6 productions as compared with piroxicam, in the present study. In addition, the combination effects of HBLKT with piroxicam were observed aftertreatment of HBLKT 1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50, 1/32ED50 and 1/64 ED50 concentrations, respectively. Results: Significant (p<0.01 or p<0.05) increases of cell viabilities and decreasesof NO, PGE2, TNF-α, IL-1β and IL-6 cytokine releases were detected in HBLKT1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50 and 1/32 ED50 concentrationco-treatment as compared with each of single 1/4 ED50 concentration of piroxicamand HBLKT treatments, respectively. Although significant (p<0.01 or p<0.05)increases of cell viabilities and decreases of NO, PGE2, TNF-α, IL-1β and IL-6cytokine releases were also demonstrated in piroxicam 1/64 ED50 + HBLKT 1/4ED50 co-treatment as compared with LPS control, no significant changes weredetected as compared with each of single 1/4 ED50 concentration of piroxicamand HBLKT treatments, in this experiment. Conclusions: Hyunbulikyung-tang showed cell protective and anti-inflammatoryeffects against LPS activated raw 264.7 cells. It, therefore, expected that HBLKTwill be showed favorable effects to relieve dysmenorrhea related to over expressedinflammatory cytokines, and it also expected that the clinical dosages of piroxicamcan be reduced as 1/8 levels as combination with HBLKT.
Abstract
Objectives: To observe the in vitro anti-inflammatory effects of Hyunbulikyung-tangaqueous extracts (HBLKT) and the possible synergic combination effects with anonsteroidal anti-inflammatory drug, piroxicam. Methods: Anti-inflammatory effects of HBLKT (yield=16.17%) were observedon LPS activated raw 264.7 cells based on ED50 to cell viability, NO, PGE2, TNF-α,IL-1β and IL-6 productions as compared with piroxicam, in the present study. In addition, the combination effects of HBLKT with piroxicam were observed aftertreatment of HBLKT 1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50, 1/32ED50 and 1/64 ED50 concentrations, respectively. Results: Significant (p<0.01 or p<0.05) increases of cell viabilities and decreasesof NO, PGE2, TNF-α, IL-1β and IL-6 cytokine releases were detected in HBLKT1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50 and 1/32 ED50 concentrationco-treatment as compared with each of single 1/4 ED50 concentration of piroxicamand HBLKT treatments, respectively. Although significant (p<0.01 or p<0.05)increases of cell viabilities and decreases of NO, PGE2, TNF-α, IL-1β and IL-6cytokine releases were also demonstrated in piroxicam 1/64 ED50 + HBLKT 1/4ED50 co-treatment as compared with LPS control, no significant changes weredetected as compared with each of single 1/4 ED50 concentration of piroxicamand HBLKT treatments, in this experiment. Conclusions: Hyunbulikyung-tang showed cell protective and anti-inflammatoryeffects against LPS activated raw 264.7 cells. It, therefore, expected that HBLKTwill be showed favorable effects to relieve dysmenorrhea related to over expressedinflammatory cytokines, and it also expected that the clinical dosages of piroxicamcan be reduced as 1/8 levels as combination with HBLKT.
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