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보허탕가감 투여가 산후 생쥐의 유즙분비량 및 유즙분비 관련 인자에 미치는 영향

Effects of Boheotang-gagam on Milk Production and Factors Related Lactation in Postpartum Mice

이창현|임지영|이은희|김홍준|이아영
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2016.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제29권 제1호|pp. 35-52|DOI http://dx.doi.org/10.15204/jkobgy.2016.29.1.035

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키워드

Boheotangaquaporin (AQP)lactationgalactagogues
주제분류한의학

초록

Objectives: The purpose of this study was to investigate the effect on factors related the expression of aquaporins (AQP) and milk production after administration of Boheotang-gagam in lactating mice. Methods: The SKH-1 mice were randomly allocated to the control group which was administered with distilled water for two weeks after the parturition and the experimental groups such as, lactating+400G group (L400G) which was administered with Boheotang-gagam 400 mg/day, lactating+600G group (L600G) which was administered with 600 mg/day for two weeks after the parturition, and 400G+lactating+400G group (400G-L400G) which was administered with 400 mg/day for 3 weeks starting one week prior to parturition for experiment (n=6 per group). Results:1. With regard to the immunohistochemical staining reaction for AQP1, AQP3, and AQP5, stronger immune response was also showed in mammary gland in all experimental groups as compared to the control group. AQP1 showed stronger immune response in the capillaries and venules which were located around the interlobular duct, while stronger immune response of AQP3 and AQP5 showed in the secretory alveolar epithelia and intralobular and interlobular ductal epithelial cells. 2. In the western blot, L400G group showed the most increased expression followed by L600G and then 400G-L400G group in AQP1. In AQP3, the order of expression density was observed as L600G, 400G-L400G and L400G group. Lastly, in AQP5, L400G group presented the most increased expression followed by L600G and 400G-L400G group. Conclusions: Boheotang-gagam would have the effect of increasing the lactation of mice after the birth by increasing the prolactin level and adjusting the expression of AQPs and prolactin receptor in the mammary glands.

Abstract

Objectives: The purpose of this study was to investigate the effect on factors related the expression of aquaporins (AQP) and milk production after administration of Boheotang-gagam in lactating mice. Methods: The SKH-1 mice were randomly allocated to the control group which was administered with distilled water for two weeks after the parturition and the experimental groups such as, lactating+400G group (L400G) which was administered with Boheotang-gagam 400 mg/day, lactating+600G group (L600G) which was administered with 600 mg/day for two weeks after the parturition, and 400G+lactating+400G group (400G-L400G) which was administered with 400 mg/day for 3 weeks starting one week prior to parturition for experiment (n=6 per group). Results:1. With regard to the immunohistochemical staining reaction for AQP1, AQP3, and AQP5, stronger immune response was also showed in mammary gland in all experimental groups as compared to the control group. AQP1 showed stronger immune response in the capillaries and venules which were located around the interlobular duct, while stronger immune response of AQP3 and AQP5 showed in the secretory alveolar epithelia and intralobular and interlobular ductal epithelial cells. 2. In the western blot, L400G group showed the most increased expression followed by L600G and then 400G-L400G group in AQP1. In AQP3, the order of expression density was observed as L600G, 400G-L400G and L400G group. Lastly, in AQP5, L400G group presented the most increased expression followed by L600G and 400G-L400G group. Conclusions: Boheotang-gagam would have the effect of increasing the lactation of mice after the birth by increasing the prolactin level and adjusting the expression of AQPs and prolactin receptor in the mammary glands.

참고문헌 (37)

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관련 논문AI추천

팔물탕 복용이 산후 유즙분비 관련 인자에 미치는 영향김태희, 송윤희대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제1호왕불유행, 목통, 통초가 산후 생쥐의 유즙분비량과 유즙분비 관련 인자에 미치는 영향김홍준, 이가위, 이은희, 이창현대한한방부인과학회 · 대한한방부인과학회지2018 · 제31권 제2호補虛湯과 補虛湯加鹿茸이 산후 생쥐의 유즙분비에 미치는 영향김태희, 이은희대한한방부인과학회 · 대한한방부인과학회지2008 · 제21권 제4호補虛湯과 補虛湯加鹿茸이 산후 생쥐의 면역반응에 미치는 영향김태희, 이은희대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제3호가미생화탕과 가미보허탕의 연속 투여가 산후 체중정체와 체성분 변화에 미치는 영향김동일, 하정일대한한방부인과학회 · 대한한방부인과학회지2007 · 제20권 제2호猪蹄와 通乳湯去穿山甲이 白鼠의 乳汁分泌에 미치는 影響김성보, 이경섭, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2004 · 제17권 제2호

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