본문 바로가기
KERD
논문KCI 미등재

Workflow of Cryo-Electron Microscopy and Status of Domestic Infrastructure

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류자연과학일반

초록

Cryo-electron microscopy (cryo-EM) allows the analysis of the near-native structures of samples such as proteins, viruses, and sub-cellular organelles at the sub-nano scale. With the recent development of analytical methods, this technique has achieved remarkable results. The importance of cryo-EM gained wide recognition due to last year’s award of the Nobel Prize in Chemistry. To help promote the knowledge of this technique, this paper introduces the basic workflows of cryo-EM and domestic cryo-EM service institutes.

Abstract

Cryo-electron microscopy (cryo-EM) allows the analysis of the near-native structures of samples such as proteins, viruses, and sub-cellular organelles at the sub-nano scale. With the recent development of analytical methods, this technique has achieved remarkable results. The importance of cryo-EM gained wide recognition due to last year’s award of the Nobel Prize in Chemistry. To help promote the knowledge of this technique, this paper introduces the basic workflows of cryo-EM and domestic cryo-EM service institutes.

참고문헌 (12)

  1. Bai X C, "Ribosome structures to near atomic resolution from thirty thousand cryo-EM particles", eLIFE, 2, pp.461~ (2013)
  2. Bammes B E, "Direct electron detection yields cryo-EM reconstructions at resolutions beyond 3", 4 Nyquist frequency, 2012, pp.177, 589~601
  3. Briggs J A, "Structural biology in situ--the potential of subtomogram averaging", Curr. Opin. Struct. Biol., 23, pp.261~267 (2013)[DOI]
  4. Carroni M, "Cryo electron microscopy to determine the structure of macromolecular complexes", Methods, 95, pp.78~85 (2016)[DOI]
  5. Danev R, "Cryo-EM single particle analysis with the volta phase plate", eLIFE, 5, pp.13046~ (2016)
  6. Dubochet J, "Cryo-electron microscopy of vitrified specimens", Q. Rev. Biophys., 21, pp.129~228 (1988)[DOI]
  7. Frank J, "Computer averaging of electron micrographs of 40S ribosomal subunits", Science, 214, pp.1353~1355 (1981)[DOI]
  8. Grassucci R A, "Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes", Nat. Protoc., 3, pp.330~339 (2008)[DOI]
  9. Li X, "Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM", Nat. Methods, 10, pp.584~590 (2013)[DOI]
  10. McMullan G, "Comparison of optimal performance at 300 keV of three direct electron detectors for use in low dose electron microscopy", Ultramicroscopy, 147, pp.156~163 (2014)[DOI]
  11. Scheres S H, "RELION: implementation of a Bayesian approach to cryo-EM structure determination", J. Struct. Biol., 180, pp.519~530 (2012)[DOI]
  12. Schur F K, "Determination of protein structure at 8.5A resolution using cryo-electron tomography and sub-tomogram averaging", J. Struct. Biol., 184, pp.394~400 (2013)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (4건)