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滋陰健脾湯加吳茱萸 추출물이 흰쥐의 뇌혈류역학에 미치는 영향

The Effect of Jaeumgeonbitang adding Evodiae Fructus Extract on the Cerebral Hemodynamics in Rats

정현우|정명수|공경희|이기승|이은경
대한예방한의학회 (Society of Preventive Korean Medicine)|2015.12|대한예방한의학회지 (Society of Preventive Korean Medicine)|제19권 제3호|pp. 155-170

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참고문헌

키워드

JaeumgeonbitangEvodiae Fructuscerebral hemodynamicscerebral blood flow
주제분류한의학

초록

Objective : Jaeumgeonbitang have been used in Korean medicine for many centuries as a therapuetic agent of vertigo. JAE was extract of Jaeumgeonbitang adding Evodiae Fructus. The effects of JAE on the cerebral blood flow and blood pressure is not known. This study was designed to investigate the effects of JAE on the ischemic crebral injuries. Method : We performed to investigate effects of JAE on the changes of regional cerebral blood flow(rCBF) and mean arterial blood pressure (MABP) in normal and ischemic rats, and further to determine the mechanism and cytokines production (IL-1β, TNF-α, IL-10, TGF-β) of JAE. Results : In normal rats, JAE significantly increased rCBF and significantly decreased MABP in a dosedependent manner. This result suggested that JAE significantly increased rCBF by dilating pial arterial diameter. Increase of JAE-induced rCBF was significantly inhibited by the pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, and was significantly inhibited by methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. Decrease of JAE-induced MABP was significantly increased by the pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. So, these results suggested that the mechanism of JAE was mediated by cyclooxygenase. In ischemic rat, the rCBF was significantly and stably increased by JAE (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group (JAE 10 ㎎/㎏ treated group) was significantly decreased IL-1β and TNF-α production compared with Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group was significantly increased IL-10 production compared with Control group. Conclusion : These results suggested that JAE was significantly and stably increased regional cerebral blood flow by inhibited IL-1β and TNF-α production, and increased IL-10 production.

Abstract

Objective : Jaeumgeonbitang have been used in Korean medicine for many centuries as a therapuetic agent of vertigo. JAE was extract of Jaeumgeonbitang adding Evodiae Fructus. The effects of JAE on the cerebral blood flow and blood pressure is not known. This study was designed to investigate the effects of JAE on the ischemic crebral injuries. Method : We performed to investigate effects of JAE on the changes of regional cerebral blood flow(rCBF) and mean arterial blood pressure (MABP) in normal and ischemic rats, and further to determine the mechanism and cytokines production (IL-1β, TNF-α, IL-10, TGF-β) of JAE. Results : In normal rats, JAE significantly increased rCBF and significantly decreased MABP in a dosedependent manner. This result suggested that JAE significantly increased rCBF by dilating pial arterial diameter. Increase of JAE-induced rCBF was significantly inhibited by the pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, and was significantly inhibited by methylene blue (10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase. Decrease of JAE-induced MABP was significantly increased by the pretreatment with indomethacin (1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. So, these results suggested that the mechanism of JAE was mediated by cyclooxygenase. In ischemic rat, the rCBF was significantly and stably increased by JAE (10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group (JAE 10 ㎎/㎏ treated group) was significantly decreased IL-1β and TNF-α production compared with Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group was significantly increased IL-10 production compared with Control group. Conclusion : These results suggested that JAE was significantly and stably increased regional cerebral blood flow by inhibited IL-1β and TNF-α production, and increased IL-10 production.

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