Effect of MEM Vitamins Supplementation of In vitro Maturation Medium and In vitro Culture Medium on the Development of Porcine Embryos
3
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was carried out to examine the influence of minimum essential medium (MEM) vitamins supplementation to in vitro maturation medium and in vitro culture medium on the development of porcine embryos. Porcine embryo development was investigated following cultivation in both in vitro maturation and culture medium with the supplementation of MEM vitamins (0, 0.1, 0.2 and 0.4%) using immature oocytes collected from the ovary of prepubertal gilts. Embryo development was observed and the total cell number in each blastocyst generated under the culture conditions was quantified following supplementation of the medium. The embryonic development rate of the blastocyst and hatched blastocyst was higher, but not significantly so, when 0.4% MEM vitamins were supplemented to the in vitro maturation medium of the porcine oocyte. Interestingly, the total number of cells in the blastocyst was significantly higher in the in vitro maturation MEM vitamins supplemented group compared to either the untreated group or the group which had MEM vitamins supplemented to both in vitro maturation and in vitro culture medium simultaneously (p<0.05). Therefore, the supplementation of 0.4% MEM vitamins to the in vitro mature medium has a beneficial effect on the embryonic development of in vitro produced blastocysts derived from the immature porcine oocytes.
Abstract
This study was carried out to examine the influence of minimum essential medium (MEM) vitamins supplementation to in vitro maturation medium and in vitro culture medium on the development of porcine embryos. Porcine embryo development was investigated following cultivation in both in vitro maturation and culture medium with the supplementation of MEM vitamins (0, 0.1, 0.2 and 0.4%) using immature oocytes collected from the ovary of prepubertal gilts. Embryo development was observed and the total cell number in each blastocyst generated under the culture conditions was quantified following supplementation of the medium. The embryonic development rate of the blastocyst and hatched blastocyst was higher, but not significantly so, when 0.4% MEM vitamins were supplemented to the in vitro maturation medium of the porcine oocyte. Interestingly, the total number of cells in the blastocyst was significantly higher in the in vitro maturation MEM vitamins supplemented group compared to either the untreated group or the group which had MEM vitamins supplemented to both in vitro maturation and in vitro culture medium simultaneously (p<0.05). Therefore, the supplementation of 0.4% MEM vitamins to the in vitro mature medium has a beneficial effect on the embryonic development of in vitro produced blastocysts derived from the immature porcine oocytes.
참고문헌 (42)
- Abeydeera, L. R, "Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa", Biol. Reprod, 57, pp.729~734 (1997)
- Abeydeera, L. R, "Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor", Theriogenology, 54, pp.787~797 (2000)
- Abeydeera, L. R., "Effect of incubation temperature on vitro maturation of porcine oocytes: Nuclear maturation, fertilization and developmental competence", Zygote, 9, pp.331~337 (2001)
- Ali, A. A, "Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development", Theriogenology, 59, pp.939~949 (2003)
- Camous, S, "Cleavage beyong the block stage and survival after transfer of early bovine embyos cultured with trophoblastic vesicles", J. Reprod. Fertil, 72, pp.479~485 (1984)
- Chang, H. S, "Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development", Mol. Reprod. Dev, 56, pp.331~335 (2000)
- Choi, Y. H, "Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome", Theriogenology, 58, pp.1187~1197 (2002)
- Dobrinsky, J. F, "Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development", Biol. Reprod, 55, pp.1069~1074 (1996)
- Eyestone, W. H, "Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue or in conditioned medium", J. Reprod. Fertil, 85, pp.710~720 (1989)
- Fukui, Y., "Effect of gonadotropins and steroids and culture on bovine oocytes maturation", Theriogenology, 18, pp.161~175 (1992)
- Hawthorne, J. N, "Inositol phospholipids. In: Phospholipids", pp.263~278 (1982), Elsevier Biomedical Press
- Heyman, Y, "In vitro cleavage of bovine and ovine early embryos: Improved development using co-culture with trophoblastic vesicles", Theriogenology, 27, pp.59~68 (1987)
- Hong, J. Y, "Effects of amino acid on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media", Theriogenology, 62, pp.1473~1482 (2004)
- Iritani, A, "Sperm peneration of pig follicular oocytes matured in culture", J. Reprod. Fertil, 54, pp.379~383 (1978)
- Kamat, J. P, "Methylene blue plus light-induced lipid peroxidation in rat liver microsomes: inhibition by nicotinamide (Vitamin B3) and other antioxidants", Chem. Biol. Interact, 99, pp.1~16 (1996)
- Kane, M. T, "The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro", J. Exp. Zool, 245, pp.220~223 (1988)
- Kane, M. T, "Vitamins requirements for development of eight-cell hamster embryos to hatching blastocysts in vitro", Biol. Reprod, 39, pp.1137~1143 (1988)
- KiKuchi, K, "Successful piglet production after transfer of blastocysts produced by a modified in vitro system", Biol. Reprod, 66, pp.1033~1041 (2002)
- Kitagawa, Y, "Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos", Theriogenology, 62, pp.1186~1197 (2004)
- Krishnaswamy, K, "Effect of vitamin B on oxygen radicals, mitochondrial membrane potential, and lipid peroxidation in H2O2-treated U937 monocytes", Free Radic. Biol. Med, 36, pp.423~428 (2004)
- Lane, M, "Amino acids and vitamins prevent culture-induced metabolic perturbation and associated loss of viability of mouse blastocysts", Hum. Reprod, 13, pp.991~997 (1998)
- Legge, M, "Free radical sacvengers ameliorate the 2-cell block in mouse embryo culture", Hum. Reprod, 6, pp.867~871 (1991)
- Lim, J. M, "Perifusion culture system for bovine embryos: improvement of embryo development by use of bovine oviduct epithelial cells, an antioxidant and polyvinyl alcohol", Reprod. Fertil. Dev, 94, pp.411~418 (1997)
- Mattioli, M., "Developemental competence of pig oocytes matured and fertilized in vitro", Theriogenology, 31, pp.1201~1207 (1989)
- May, J. V., "Differential effects of epidermal growth factor, somatomedian-C", insulinlike growth factor and transforming growth factor-beta on porcine granulose cell DNA synthesis and cell proliferation, 1988, pp.123, 168~179
- McKiernen, S. H, "Culture of one-cell hamster embryos with wate soluble vitamins: pantothenate stimulates blastocyst production", Hum. Reprod, 15, pp.157~164 (2000)
- McDowell, L. R, "Vitamins in animal and human nutrition" (2000), Iowa State University Press
- Mishra, A, "Successful developoment in vitro of hamster 8-cell embryos to ‘zona-escaped’ and attached blastocysts: assessment of qulaity and trophoblast outgrowth", Reprod. Fertil. Dev, 10, pp.413~420 (1998)
- Motlik, J., "Meiotic competence in vitro of pig oocytes isolated from early antral follicles", J. Reprod. Fertil, 72, pp.323~328 (1984)
- Murray, R. K, "Lipid peroxidation is a source of free radicals in vitro", Harper's Biochem, 20, pp.142~143 (1990)
- Nagai, T, "In vitro fertilization of pig oocytes by frozen boar spermatozoa", J. Reprod. Fertil, 84, pp.585~591 (1988)
- Ogawa, T., "The effect of serum fractions on single-cell mouse embryos in vitro", J. In vitro Fertil. Embryo. Transfer, 4, pp.153~158 (1987)
- Olson, S. E, "Culture of in vitroproduced bovine embryos with vitamin E improves development in vitro and after transfer to recipients", Biol. Reprod, 62, pp.248~252 (2000)
- Prather, R, "Practical consideration for the in vitro production of pig embryos. Theriogenology 49:23-32. Petters, R. M. and K. D. Wells. 1993. Culture of pig embryos", J. Reprod. Fertil. Suppl, 48, pp.61~73 (1993)
- Rizos, D. A., "Bovine embryos culture in the presence or absence of serum implication for blastocyst development, cryotolerance, and messenger RNA expression", Biol. Reprod, 68, pp.236~243 (2003)
- Saito, H., "The effect of serum fractions on embryo growth", Fertil. Steril, 41, pp.761~765 (1984)
- Stokes, P. J., "Development of porcine embryos in vivo and in vitro; evidence for embryo ‘cross talk’ in vitro", Dev. Biol, 284, pp.62~71 (2005)
- Stryer, L, "Biochemistry", pp.322~325 (1988), W. H. Freeman
- Vance, D. E, "Phospholipid metabolism in eukaryotes. In: Biochemistry of lipids and membranes", pp.1986, The Benjamin
- Wang, W. H., "Induction of cortical granule exocytosis of pig oocytes by spermatozoa during meiotic maturation", J. Reprod. Fertil, 109, pp.247~255 (1997)
- Xu, X. J., "Development of in vitro fertilized bovine ova in two co-culture systems", Anim. Reprod, 1, pp.372~374 (1992)
- Yoshioka, K, "Birth of piglets derived from porcine zygotes cultured in a chemically defined medium", Biol. Reprod, 66, pp.112~119 (2002)
