Effect of Alpha-Linolenic Acid on Oocyte Maturation and Embryo Development in Pigs
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and 100 μM) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with 50 μM ALA were fertilized and cultured in IVC medium with ALA (25, 50 and 100 μM) during early-embryogenesis (48 hours after fertilization). Then, embryos were cultured with 25 μM ALA during early embryogenesis and/or late embryogenesis (120 hours after early-embryogenesis). In results, oocyte maturation were significantly increased by 50 μM ALA treatment groups compared with control groups (p<0.05). Treatment of 25 μM ALA during early-embryogenesis enhanced cleavage rate of em-bryo compared with other groups (p<0.05), whereas formation and total cell number of blastocyst had no significant differ-ence. Similarly, cleavage rate of embryos were increased by 25 μM ALA supplement during early- or late-embryogenesis than ALA treatment both stage of embryogenesis (p<0.05), but did not influence to blastocyst formation. Interestingly, total cell number of blastocyst were enhanced in ALA treatment group during early-embryogenesis. These findings indicated that ALA supplement enhance the nuclear maturation of oocyte and embryo development, however, excessive ALA could negatively in-fluence. Therefore, we suggest that ALA is used for improvement of in vitro production of mammalian embryo and further study regarding with functional mechanism of ALA is needed
Abstract
The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and 100 μM) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with 50 μM ALA were fertilized and cultured in IVC medium with ALA (25, 50 and 100 μM) during early-embryogenesis (48 hours after fertilization). Then, embryos were cultured with 25 μM ALA during early embryogenesis and/or late embryogenesis (120 hours after early-embryogenesis). In results, oocyte maturation were significantly increased by 50 μM ALA treatment groups compared with control groups (p<0.05). Treatment of 25 μM ALA during early-embryogenesis enhanced cleavage rate of em-bryo compared with other groups (p<0.05), whereas formation and total cell number of blastocyst had no significant differ-ence. Similarly, cleavage rate of embryos were increased by 25 μM ALA supplement during early- or late-embryogenesis than ALA treatment both stage of embryogenesis (p<0.05), but did not influence to blastocyst formation. Interestingly, total cell number of blastocyst were enhanced in ALA treatment group during early-embryogenesis. These findings indicated that ALA supplement enhance the nuclear maturation of oocyte and embryo development, however, excessive ALA could negatively in-fluence. Therefore, we suggest that ALA is used for improvement of in vitro production of mammalian embryo and further study regarding with functional mechanism of ALA is needed
참고문헌 (28)
- Aitken RJ, "Cis-unsaturated fatty acids stimu-late reactive oxygen species generation and lipid per-oxidation in human spermatozoa", J Clin Endocrinol Metab, 91, pp.4154~4163 (2006)
- Al-Gubory KH, "The role of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes", Int J Biochem Cell Biol, 42, pp.1634~1650 (2010)
- Ambrose DJ, "Lower pregnancy losses in lactating dairy cows fed a diet enriched in alpha-linolenic acid", J Dairy Sci, 89, pp.3066~3074 (2006)
- Bilby TR, "Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in sum-mer", J Dairy Sci, 89, pp.3891~3903 (2006)
- Elahi F, "Cilostazol improves developmental competence of pig oocytes by increasing intraoocyte cyclic adeno-sine monophosphate level and delaying meiotic re-sumption", Reprod Domest Anim, 51, pp.220~226 (2016)
- Fouladi-Nashta AA, "Oo-cyte quality in lactating dairy cows fed on high levels of n-3 and n-6 fatty acids", Reproduction, 138, pp.771~781 (2009)
- Guerin P, "Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings", Hum Reprod, 7, pp.175~189 (2001)
- Homa ST, "Changes in linoleic acid dur-ing follicular development and inhibition of spontane-ous breakdown of germinal vesicles in cumulus-free bovine oocytes", J Reprod Fertil, 94, pp.153~160 (1992)
- Kątska-Książkiewicz L, "Pig embryo production by in vitro maturation and fertilization of ovarian oocytes. A review", J Anim Feed Sci, 15, pp.525~542 (2006)
- Khalil WA, "Protective effects of antioxidants on linoleic acid–treated bovine oocytes during maturation and subsequent embryo develop-ment", Theriogenology, 80, pp.161~168 (2013)
- Khandoker M, "Metabolism of exogenous fatty acids by preimplantation rabbit embryos", Jpn J Fertil Steril, 43, pp.195~201 (1998)
- 김정훈, "The Effect of Fibroblast Co-culture on In Vitro Maturation of Mouse Preantral Follicles", 발생과 생식, 17(3), pp.269~274 (2013), 한국발생생물학회
- Kitagawa Y, "Ef-fects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in por-cine embryos", Theriogenology, 62, pp.1186~1197 (2004)
- Kowaltowski AJ, "Mitochondrial dam-age induced by conditions of oxidative stress", Free Radic Biol Med, 26, pp.463~471 (1999)
- Lee S, "Sequential treatment with resveratrol-trolox improves development of porcine embryos derived from parthe-nogenetic activation and somatic cell nuclear transfer", Theriogenology, 84, pp.145~154 (2015)
- Lee Y, "Alpha-linolenic acid treatment during oocyte maturation enhances embryonic devel-opment by influencing mitogen-activated protein ki-nase activity and intraoocyte glutathione content in pigs", J Anim Sci, 94, pp.3255~3263 (2016)
- Lin L, "Osmo-tic stress induced by sodium chloride, sucrose or treha-lose improves cryotolerance and developmental com-petence of porcine oocytes", Reprod Fertil Dev, 21, pp.338~344 (2009)
- Marei WF, "The effect of linolenic acid on bovine oocyte maturation and development", Biol Reprod, 81, pp.1064~1072 (2009)
- Marei WF, "Differ-ential effects of linoleic and alpha-linolenic fatty acids on spatial and temporal mitochondrial distribution and activity in bovine oocytes", Reprod Fertil Dev, 24, pp.679~690 (2012)
- McKeegan PJ, "The role of fatty acids . in oocyte and early embryo development", Reprod Fertil Dev, 24, pp.59~67 (2011)
- Nagai T, "The improvement of in vitro maturation systems for bovine and porcine oocytes", Theriogenolo-gy, 55, pp.1291~1301 (2001)
- Pratt SL, "Factors influencing the commercialisation of cloning in the pork industry", J Reprod Fertil Suppl, 62, pp.303~315 (2006)
- Sturmey RG, "Role of fatty acids in energy provision during oocyte matu-ration and early embryo development", Reprod Domest Anim, 44, pp.50~58 (2009)
- Takahashi M, "Oxidative stress and redox regulation on in vitro development of mammalian embryos", J Re-prod Dev, 58, pp.1~9 (2012)
- Wakefield SL, "Maternal supply of omega-3 polyunsaturated fatty acids alter mechanisms involved in oocyte and early embryo development in mouse", Am J Physiol Endocrinol Metab, 294, pp.E425~E434 (2007)
- Wathes DC, "Polyun-saturated fatty acids in male and female reproduction", Biol Reprod, 77, pp.190~201 (2007)
- Wonnacott KE, "Dietary omega-3 and-6 polyunsaturated fatty acids affect the composi-tion and development of sheep granulosa cells, oocytes and embryos", Reproduction, 139, pp.57~69 (2010)
- Wu Y, "Citrinin exposure affects oocytes matu-ration and embryo development by inducing oxidative stress-mediated apoptosis", Oncotarget, 8, pp.34525~34533 (2017)