Anti-obese related pharmacological effects of standard potato protein extracts on the 45%Kcal high fat diet supplied mice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives : In present study, therefore, possible beneficial pharmacological activities of standard potato protein extracts (SPE) were observed on the mild diabetic obese mice. Methods : After end of 12 weeks of continuous oral administrations of three different dosages of SPE 400, 200 and 100 mg/kg, or metformin 250 mg/kg, analyzed the hepatoprotective, hypolipidemic, hypoglycemic, nephroprotective and anti-obesity effects, separately. In addition, liver antioxidant defense systems were additionally measured with lipid metabolism-related genes expressions and hepatic glucose-regulating enzyme activities for action mechanism. Results : All of diabetes and related complications including obesity were significantly inhibited by treatment of SPE 400, 200 and 100 mg/kg, dose-dependently, and they also dramatically normalized the hepatic lipid peroxidation and depletion of liver endogenous antioxidant defense system, the changes of the hepatic glucose-regulating enzyme activities, also changes of the lipid metabolism-related genes expressions including hepatic AMPKα1 and AMPKα2 mRNA expressions, dose-dependently. Especially, SPE 200 mg/kg constantly showed favorable inhibitory activities against type II diabetes and related complications as comparable to those of metformin 250 mg/kg in HFD mice, respectively. Conclusions : The present work demonstrated that SPE 400, 200 and 100 mg/kg showed favorable anti-diabetic and related complications including obesity refinement activities in HFD mice, through AMPK upregulation mediated hepatic glucose enzyme activity and lipid metabolism-related genes expression, antioxidant defense system and pancreatic lipid digestion enzyme modulatory activities.
Abstract
Objectives : In present study, therefore, possible beneficial pharmacological activities of standard potato protein extracts (SPE) were observed on the mild diabetic obese mice. Methods : After end of 12 weeks of continuous oral administrations of three different dosages of SPE 400, 200 and 100 mg/kg, or metformin 250 mg/kg, analyzed the hepatoprotective, hypolipidemic, hypoglycemic, nephroprotective and anti-obesity effects, separately. In addition, liver antioxidant defense systems were additionally measured with lipid metabolism-related genes expressions and hepatic glucose-regulating enzyme activities for action mechanism. Results : All of diabetes and related complications including obesity were significantly inhibited by treatment of SPE 400, 200 and 100 mg/kg, dose-dependently, and they also dramatically normalized the hepatic lipid peroxidation and depletion of liver endogenous antioxidant defense system, the changes of the hepatic glucose-regulating enzyme activities, also changes of the lipid metabolism-related genes expressions including hepatic AMPKα1 and AMPKα2 mRNA expressions, dose-dependently. Especially, SPE 200 mg/kg constantly showed favorable inhibitory activities against type II diabetes and related complications as comparable to those of metformin 250 mg/kg in HFD mice, respectively. Conclusions : The present work demonstrated that SPE 400, 200 and 100 mg/kg showed favorable anti-diabetic and related complications including obesity refinement activities in HFD mice, through AMPK upregulation mediated hepatic glucose enzyme activity and lipid metabolism-related genes expression, antioxidant defense system and pancreatic lipid digestion enzyme modulatory activities.
참고문헌 (67)
- Wendel AA, "Conjugated linoleic acid fails to worsen insulin resistance but induces hepatic steatosis in the presence of leptin in ob", ob mice, 2008, pp.49(1), 98~106
- Tilg H, "Adipocytokines: mediators linking adipose tissue, inflammation and immunity", Nature reviews Immunology, 6(10), pp.772~783 (2006)[DOI]
- James PT, "The worldwide obesity epidemic", Obesity research, 9(Suppl 4), pp.228S~233S (2001)[DOI]
- Zimmet P, "The burden of type 2 diabetes: are we doing enough?", Diabetes & metabolism, 29(4), pp.6S9~6S18 (2003)
- Kunitomi M, "Relationship between reduced serum IGF-I levels and accumulation of visceral fat in Japanese men", International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, 26(3), pp.361~369 (2002)[DOI]
- Hida K, "Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity", Proceedings of the National Academy of Sciences of the United States of America, 102(30), pp.10610~10615 (2005)[DOI]
- German Diabetes Association, "2001", 109(Suppl 2), pp.10.1055, s-2001-18576
- Goldstein BJ, "Insulin resistance as the core defect in type 2 diabetes mellitus", The American journal of cardiology, 90(5A), pp.3G~10G (2002)
- Angulo P, "Nonalcoholic fatty liver disease", The New England journal of medicine, 346(16), pp.1221~1231 (2002)[DOI]
- Kadowaki T, "Adiponectin and adiponectin receptors", Endocrine reviews, 26(3), pp.439~451 (2005)[DOI]
- Inzucchi SE, "Oral antihyperglycemic therapy for type 2 diabetes: scientific review", Jama, 287(3), pp.360~372 (2002)[DOI]
- Kang SJ, "Fermentation with Aquilariae Lignum enhances the anti-diabetic activity of green tea in type II diabetic db", db mouse, 2014, pp.6(9), 3536~3571
- Jung YM, "Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects", Nutrition research (New York, NY), 31(5), pp.387~396 (2011)[DOI]
- Kim CM, "Red-koji fermented red ginseng ameliorates high fat diet-induced metabolic disorders in mice", Nutrients, 5(11), pp.4316~4332 (2013)[DOI]
- Choi JS, "Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes", International journal of molecular medicine, 39(6), pp.1437~1451 (2017)[DOI]
- Chen H, "Physicochemical properties and antioxidant capacity of 3 polysaccharides from green tea, oolong tea, and black tea", Journal of food science, 74(6), pp.C469~C474 (2009)[DOI]
- Hays NP, "Prevention and treatment of type 2 diabetes: current role of lifestyle, natural product, and pharmacological interventions", Pharmacology & therapeutics, 118(2), pp.181~191 (2008)[DOI]
- Elkahoui S, "Dietary Supplementation of Potato Peel Powders Prepared from Conventional and Organic Russet and Non-organic Gold and Red Potatoes Reduces Weight Gain in Mice on a High-Fat Diet", Journal of agricultural and food chemistry, 66(24), pp.6064~6072 (2018)[DOI]
- Ju R, "Purple Sweet Potato Attenuate Weight Gain in High Fat Diet Induced Obese Mice", Journal of food science, 82(3), pp.787~793 (2017)[DOI]
- Kim MH, "Purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "Golden Valley"", Biochemical and biophysical research communications, 346(3), pp.681~686 (2006)[DOI]
- Komarnytsky S, "Potato protease inhibitors inhibit food intake and increase circulating cholecystokinin levels by a trypsin-dependent mechanism", International journal of obesity (2005), 35(2), pp.236~243 (2011)[DOI]
- Ku SK, "Anti-obesity and anti-diabetic effects of a standardized potato extract in ob", ob mice, 2016, pp.12(1), 354~364
- Lee DG, "In vitro antioxidant and anti-adipogenic effects of slendesta, standard potato extracts containing 5% protease inhibitor II", African Journal of Traditional, Complementary and Alternative Medicines, 13(2), pp.164~175 (2016)[DOI]
- Haluzik M, "Genetic background (C57BL", 6J versus FVB, ob mice, pp.Endocrinology, 2004[DOI]
- Lin HZ, "Metformin reverses fatty liver disease in obese, leptin-deficient mice", Nature medicine, 6(9), pp.998~1003 (2000)[DOI]
- Kurundkar SB, "Effect of a novel biphenyl compound, VMNS2e on ob", ob mice, 2011, pp.650(1), 472~478
- Plummer MR, "Atherosclerotic lesion formation and triglyceride storage in obese apolipoprotein AI-deficient mice", The Journal of nutritional biochemistry, 19(10), pp.664~673 (2008)[DOI]
- Margalit M, "Glucocerebroside ameliorates the metabolic syndrome in OB", OB mice, 2006, pp.319(1), 105~110
- Sone H, "Disease model: hyperinsulinemia and insulin resistance. Part A-targeted disruption of insulin signaling or glucose transport", Trends in molecular medicine, 7(7), pp.320~322 (2001)
- Park SH, "Euonymus alatus prevents the hyperglycemia and hyperlipidemia induced by high-fat diet in ICR mice", Journal of ethnopharmacology, 102(3), pp.326~335 (2005)[DOI]
- Ma A, "AMPK activation enhances the anti-atherogenic effects of high density lipoproteins in apoE-", - mice, 2017, pp.58(8), 1536~1547
- Korea Food and Drug Administration, "Testing Guidelines for Safety Evaluation of Drugs", ~ (2015)
- Lee JE, "Fermentation of Green Tea with 2% Aquilariae lignum Increases the Anti-Diabetic Activity of Green Tea Aqueous Extracts in the High Fat-Fed Mouse", Nutrients, 7(11), pp.9046~9078 (2015)[DOI]
- Chung SI, "Comparative study on the hypoglycemic and antioxidative effects of fermented paste (doenjang) prepared from soybean and brown rice mixed with rice bran or red ginseng marc in mice fed with high fat diet", Nutrients, 6(10), pp.4610~4624 (2014)[DOI]
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", The Journal of biological chemistry, 226(1), pp.497~509 (1957)
- Lowry OH, "Protein measurement with the Folin phenol reagent", The Journal of biological chemistry, 193(1), pp.265~275 (1951)
- Sun Y, "A simple method for clinical assay of superoxide dismutase", Clinical chemistry, 34(3), pp.497~500 (1988)
- Hulcher FH, "Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A", Journal of lipid research, 14(6), pp.625~631 (1973)
- Davidson AL, "Factors underlying significant underestimations of glucokinase activity in crude liver extracts: physiological implications of higher cellular activity", Archives of biochemistry and biophysics, 253(1), pp.156~167 (1987)[DOI]
- Alegre M, "Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction", Analytical biochemistry, 173(1), pp.185~189 (1988)[DOI]
- Bentle LA, "Interaction of anions and divalent metal ions with phosphoenolpyruvate carboxykinase", The Journal of biological chemistry, 251(10), pp.2916~2921 (1976)
- Sung YY, "Anti-obesity activity, acute toxicity, and chemical constituents of aqueous and ethanol Viola mandshurica extracts", BMC complementary and alternative medicine, 17(1), pp.297~ (2017)[DOI]
- Schmittgen TD, "Analyzing real-time PCR data by the comparative CT method", Nature protocols, 3(6), pp.1101~1108 (2008)[DOI]
- Kawakami S, "Effects of dietary supplementation with betaine on a nonalcoholic steatohepatitis (NASH) mouse model", Journal of nutritional science and vitaminology, 58(5), pp.371~375 (2012)[DOI]
- Fujita H, "Effects of antidiabetic treatment with metformin and insulin on serum and adipose tissue adiponectin levels in db", db mice, 2005, pp.52(4), 427~433
- Mitchell M, "Adipokines: implications for female fertility and obesity", Reproduction (Cambridge, England), 130(5), pp.583~597 (2005)[DOI]
- Wilson JS, "Combined effects of protein deficiency and chronic ethanol consumption on rat pancreas", Digestive diseases and sciences, 33(10), pp.1250~1259 (1988)[DOI]
- Gartner LP, "Color Textbook of Histology. 3rd ed" (2007), Saunders
- Snedeker SM, "Do interactions between gut ecology and environmental chemicals contribute to obesity and diabetes?", Environmental health perspectives, 120(3), pp.332~339 (2012)[DOI]
- Larsen ML, "Effect of long-term monitoring of glycosylated hemoglobin levels in insulin-dependent diabetes mellitus", The New England journal of medicine, 323(15), pp.1021~1025 (1990)[DOI]
- Terauchi Y, "Glucokinase and IRS-2 are required for compensatory beta cell hyperplasia in response to high-fat diet-induced insulin resistance", The Journal of clinical investigation, 117(1), pp.246~257 (2007)[DOI]
- Noriega-Lopez L, "Pancreatic insulin secretion in rats fed a soy protein high fat diet depends on the interaction between the amino acid pattern and isoflavones", The Journal of biological chemistry, 282(28), pp.20657~20666 (2007)[DOI]
- Neves RH, "Hepatic stereology of Schistosomiasis mansoni infected-mice fed a high-fat diet", Memorias do Instituto Oswaldo Cruz, 101(Suppl 1), pp.253~260 (2006)[DOI]
- Quine SD, "Effects of (-)-epicatechin, a flavonoid on lipid peroxidation and antioxidants in streptozotocin-induced diabetic liver, kidney and heart", Pharmacological reports : PR, 57(5), pp.610~615 (2005)
- Sodikoff C, "Laboratory Profiles of Small Animal Diseases: A Guide to Laboratory Diagnosis" (1995), Mosby
- Garg MC, "Effect of vitamin C supplementation on oxidative stress in experimental diabetes", Indian journal of experimental biology, 35(3), pp.264~266 (1997)
- Ceriello A, "New insights on non-enzymatic glycosylation may lead to therapeutic approaches for the prevention of diabetic complications", Diabetic medicine : a journal of the British Diabetic Association, 9(3), pp.297~299 (1992)[DOI]
- Maiese K, "New Insights for Oxidative Stress and Diabetes Mellitus", Oxidative medicine and cellular longevity, 2015, pp.875961~ (2015)
- Akbel E, "The subchronic exposure to malathion, an organophosphate pesticide, causes lipid peroxidation, oxidative stress, and tissue damage in rats: the protective role of resveratrol", Toxicology research, 7(3), pp.503~512 (2018)[DOI]
- Jung UJ, "Supplementation of persimmon leaf ameliorates hyperglycemia, dyslipidemia and hepatic fat accumulation in type 2 diabetic mice", PloS one, 7(11), pp.e49030~ (2012)[DOI]
- Wu D, "Ameliorative effect of berberine on renal damage in rats with diabetes induced by high-fat diet and streptozotocin", Phytomedicine : international journal of phytotherapy and phytopharmacology, 19(8-9), pp.712~718 (2012)[DOI]
- Wu D, "Exogenous hydrogen sulfide mitigates the fatty liver in obese mice through improving lipid metabolism and antioxidant potential", Medical gas research, 5(1), pp.1~ (2015)[DOI]
- Coope GJ, "Predictive blood glucose lowering efficacy by Glucokinase activators in high fat fed female Zucker rats", British journal of pharmacology, 149(3), pp.328~335 (2006)[DOI]
- van Schaftingen E, "The glucose-6-phosphatase system", The Biochemical journal, 362, pp.513~532 (2002)[DOI]
- Lin CH, "Effects of Bofu-Tsusho-San on diabetes and hyperlipidemia associated with AMP-activated protein kinase and glucose transporter 4 in high-fat-fed mice", International journal of molecular sciences, 15(11), pp.20022~20044 (2014)[DOI]
- Dulloo AG, "Adaptive thermogenesis and uncoupling proteins: a reappraisal of their roles in fat metabolism and energy balance", Physiology & behavior, 83(4), pp.587~602 (2004)[DOI]
- Wu X, "Involvement of AMP-activated protein kinase in glucose uptake stimulated by the globular domain of adiponectin in primary rat adipocytes", Diabetes, 52(6), pp.1355~1363 (2003)[DOI]