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돼지 황체에서 TNFα？분비 대식세포와 내피세포에 관한 면역조직화학적 연구

Immunohistochemical Study on the TNFα-Secreting Macrophages and Endothelial Cells in the Porcine Corpus Luteum

김원식|김수일|박창식|조근자|한승로
한국축산학회 (Korean Society of Animal Sciences and Technology)|2005.10|한국축산학회지 (Journal of Animal Science and Technology)|제47권 제5호|pp. 711-720

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참고문헌

주제분류축산학

초록

황체내 TNFα는 큰황체세포를 포함한 황체내 세포들의 사망을 일으키고, 황체내 혈관의 퇴화와 프로게스테론 농도의 감소를 초래하는 등 황체의 구조적 및 기능적 퇴화를 유발하는 것으로 밝혀졌다. 반면 이와는 대조적으로 TNFα는 황체발달의 초기에는 황체자극효과를 가지고 있다는 보고도 있다. 황체내에서 TNFα의 중요성에도 불구하고, 아직 이 물질의 분비원이 대식세포라는 보고들과 대식세포는 주된 분비원이고 내피세포에서도 소량 분비된다는 보고 등 이견이 있다. 이를 알아보기 위해 저자들은 돼지에서 비임신기와 임신기의 성숙황체와 용해황체를 이용하여 대식세포와 TNFα 면역조직화학을 실시한 결과, 성숙황체내에서 TNFα의 주된 분비원은 대식세포이고 내피세포도 일부 분비하는 것을 확인하였으며, 황체용해가 진행되면서 내피세포가 TNFα의 분비와 반응에 다양성과 역동성을 보이고 있음을 추정할 수 있었다.

Abstract

In the corpus luteum, TNFα is known to induce functional and structural luteolysis. In addition, it acts as luteotropic agent during the initial and early stage of luteal development. In spite of its importance in corpus luteal development, there is still different opinions for the source cells of TNFα in the corpus luteum. One is the macrophages only, and the other is macrophages are the main source and endothelial cells are the minor source. In this experiment, using the porcine corpora lutea of pregnancy and ovulatory stages, hematoxylin-eosin stain, macrophage and TNFα immunohistochemistry were carried to reveal the sources of TNFα. As a result, MAC 387-positive macrophages were present in all the stages of corpora lutea. In the mature corpora lutea of nonpregnant stages, the sites of MAC 387-positive macrophages and those of TNFα- positive macrophages were coincided, and the sites of endothelial cells and those of TNFα-positive endothelial cells were nearly coincided. But, in the mature CL of pregnant stage, mid- and advanced luteolytic stages of both nonpregnant and pregnant stages, the sites of MAC 387-positive macrophages and those of TNFα-positive macrophages were coincided, but not in the endothelial cells. Accordingly, it can be concluded that macrophages are the main source of TNFα in the corpus luteum and endothelial cells are the minor source in the mature and mid-lytic stages, but, in the advanced luteolytic stage, macrophages are the only source of TNFα.

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