Quercetin의 급여가 산양의 사료이용성, 혈액상 및 육질에 미치는 영향
Effects of Dietary Quercetin on the Feed Utilization, Blood Parameters, and Meat Quality in Korean Native Goats
1
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
본 실험은 quercetin의 급여가 우리나라 재래 산양의 사료이용성, 혈액 성상 및 육질에 미치는 영향을 조사하고자 실시하였으며, 공시동물로 체중 15kg 정도의 우리나라 재래산양 16두(2처리 8반복)를 공시하여 TMR 사료에 quercetin을 0 및 200mg/kg씩 첨가하여 사양실험을 실시하였다. 실험결과로서, 재래산양의 사료섭취량, 음수량, 배뇨량 및 배분량은 유의한 차이가 없었으며, 조지방, NDF 및 ADF의 소화율도 처리간에 유의한 차이가 없었으나, 건물 및 조단백질의 소화율은 첨가구가 무첨가구에 비하여 유의하게 높은 결과였다(P<0.05). 그리고 반추위액의 pH는 처리 간에 유의한 차이가 없었고, total VFA, 프로피온산 및 낙산의 농도는 첨가구가 유의하게 높았다(P<0.05). 그러나 초산, 이소 낙산, 이소 발레린산 및 발레린산은 처리구간에 유의한 차이가 없었다. A/P비는 첨가구가 유의하게 낮게 나타났다. BUN․BUN/ creatinine은 quercetin 첨가구가 무첨가구에 비하여 유의하게 높았으나, ALT․AST․Creatinine․Albumin․γ-GT․Ca․Pi의 농도는 유의한 영향을 관찰할 수 없었다. 재래산양육의pH, 보수력 및 총 페놀 함량은 유의적인 차이를 보이지 않았으며, ABTS+radical소거 능력은 첨가구가 무첨구에 비하여 도축 후 0시간에서 유의하게 높게 나타났으나 일수가 경과함에 따라 유의한 차이는 없어졌다. 관능검사에서는 quercetin을 섭취한 산양의 육에서 색과, 조직감과 전반적인 부분의 선호도에서 유의적으로 높은 결과를 보였다. 결론적으로 건물과 조단백질 소화율, 반추위 발효성상 및 관능검사에서 긍정적인 결과를 얻었지만 육질 평가에 있어 pH, 보수력, 전자공여능 및 지방산패도에 유의적인 차이를 보이지 않았다. 그러나 재래산양육의 ABTS 환원력이나 관능적 특성에서 긍정적인 결과를 나타내어 quercetin 급여가 육질 향상에 효과적일 수 있는 가능성은 제시되었다. 따라서 추후 quercetin 급여가 육질에 미치는 영향에 대한 지속적인 연구가 필요하다고 하겠다.
Abstract
This study was conducted to investigate the effects of dietary quercetin on feed utilization, blood parameters, and meat quality of Korean native goats. Totally sixteen Korean native goats, 15kg of average BW aged at 7 months, were employed in the experiment with eight replicates per treatment. One group was fed quercetin at 200mg/kg level and the other group was fed none as control for 15 days. Dietary inclusion of quercetin did not affect feed intake, water intake, and the amount of urine and feces. Digestibilities of crude fat, NDF, and ADF for 5 days were not affected, but digestibility of crude protein was increased by the dietary inclusion of qurecetin(P<0.05). Quercetin increased rumen total VFA, propionate, and butyrate significantly (P<0.05). Acetate/propionate ratio(A/P) in the quercetin treated group was significantly higher than control. 2,2-Azinobis-(3- ethylbenzothiazoline-6-sulfonic acid)(ABTS+) reducing activity of the loin from goat fed quercetin was higher than that of control. Sensory analysis conducted at 24 hr post mortem revealed that color, texture, and overall acceptability of the loin from goat fed quercetin were significantly preferred to that of control. Feeding quercetin did not influence pH, water holding capacity, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, TBARS value, and fatty acid composition of the loin significantly. In conclusion, the dietary inclusion of quercetin increased the digestibility of crude protein, rumen total VFA, propionate, butyrate, and A/P ratio. In addition the higher color and texture preference and ABTS+reducing activity of loin indicating some beneficial effect on enhancement of meat qualityin goats.
참고문헌 (33)
- Ahn, D. U., "Volatiles production and lipid oxidation on irradiated cooked sausage as related to packaging storage", J. Food. Sci., 64, pp.226~229 (1999)
- Ahn, S. I., "Antioxidative activity and nitrite-scavenging abilities of some phenolic compounds", Kor J. Food Cookery Sci., 23, pp.19~24 (2007)
- AOAC, "Official Method of Analysis.(16th ed.)" (1995), Association of Official Analytical Chemists
- Botsoglou, N., "The incorporation of dehydrated rosemary leaves in the rations of turkey and their impact on the oxidative stability of the produced raw and cooked meat", Int J Food Sci Nutr., 58, pp.312~320 (2007)
- Blois, M. S., "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1190~1200 (1958)
- Chen, Y. C., "Flavone inhibition of tumor growth via apoptosis in vitro and in vivo", Int. J. Oncol., 25, pp.661~670 (2004)
- Daniel, R. S., "Mechanism of action of antiatherogenic and related effects of Ficus bengalensis Linn. flavonoids in experimental animals", Indian J. Exp. Biol., 41, pp.296~303 (2003)
- Duarte, J., "Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats", Br. J. Pharmacol, 133, pp.117~124 (2001)
- Edenharder, R., "Free radical scavenging abilities of flavonoids as mechanism of protection against mutagenicity induced by tert-butyhydroperoxide or cumene hydro- peroxide in Salmonella typhimurium TA102", Mut. Res., 9, pp.1~18 (2003)
- Erel, O., "A novel automated direct measurement method for total antioxidant capacity using a new generation, more atable ABTS+ Radical cation", Clin. Biochem., 37, pp.277~285 (2004)
- Erwin, E. S., "Volatile fatty acid analysis of blood and rumen fluid by gas chromatography", J. Anim. Sci., 44, pp.1768~ (1961)
- Farombi, E. O., "Monosodium glutamate- induced oxidative damage and genotoxicity in the rat: modulatory role of vitamin C, vitamin E and quercetin", Human & Experimental Toxicology, 25, pp.251~259 (2006)
- Fenner, H., "Quantitative method for determining the steam volatile fatty acids in rumen fluid by gas chromatography", J. Dairy Sci., 22, pp.624~ (1963)
- Gladine, C., "Plant extracts rich in polyphenols(PERP) are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n-3 PUFA supplemented diets", Anim Feed Sci Technol, 136, pp.281~296 (2007)
- Goering, H. K., "Forage fiber analysis" (1970), ARS, USDA Agric
- Herbert, A., "Sensory evaluation practices. 2nd ed.", pp.68~75 (1993), Academic Press
- 현선희, "제주 자생식물로부터 항산화 및 화장품 기능성 소재 탐색", 한국식품과학회지, 39(2), pp.200~208 (2007), 한국식품과학회
- Janbaz, K. H., "Studies on the protective effects of caffeic acid and quercetin on chemical- induced hepatotoxicity in rodents", Phytomedicine, 11, pp.424~430 (2004)
- Jang, A., "Antioxidative potential of raw breast meat from brioler chicks fed a dietary medicinal herb extract mix", Poult Sci, 87, pp.2382~2389 (2008)
- Kahraman, A., "Protective effects of quercetin on ultraviolet a light-induced oxidative stress in the blood of rat", J. Appl. Toxicol., 22, pp.303~309 (2002)
- Effect of Dietary Quercetin on Pork Quality, "Kremer, B. T.", ~, pp.Iowa State University ASL-1621 (1999)
- Lopez-Bote, C. J., "Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat", Brit Poult Sci, 39, pp.235~240 (1998)
- Psotova, J., "Chemoprotective effects of plant phenolics against anthracyclineinduced toxicity on rat cardiomyocytes: Part III. Apigenin, baicalein, kaempherol, luteolin and quercetin", Phytother. Res., 18, pp.516~521 (2004)
- Rice-Evans, C., "Structure-antioxidant activity relationships of flavonoids and isoflavonoids", Flavonoidsin Health and Disease, pp.199~219 (1998)
- Santas, J., "Compraison of the antioxidant activity of two Spanish onion varieties", Food Chem., 107, pp.1210~1216 (2008)
- Simitzis, P. E., "Effect of dietary oregano supplementation on lamb meat characteristics", Meat Sci., 79, pp.217~223 (2008)
- Singleton, V. L., "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", AM. J. Enol. Vitic., 29, pp.144~158 (1965)
- Soloviev, A., "Arrhythmogenic peroxynitrite-induced alterations in mammalian heart contractility and itsprevention with quercetin-filled liposome", Cardiovasc. Toxicol., 2, pp.129~139 (2002)
- Vichi, S., "Determination of the presence of antioxidants deriving from sage and oregano extracts added to animal fat by means of assessment of the radical scavenging capacity by photo- chemiluminescence analysis", Nahrung, 2001, pp.45, 101~104
- Vincent, C. J. B., "Tissue Distribution of Quercetin in Rats and Pigs", J. Nutr., 135, pp.1617~1618 (2005)
- 박관하, "Mouse에서의 quercetin 경구투여 후의 체내 농도 및 대사체 isorhamnetin의 농도변화", 한국식품과학회지, 37(1), pp.90~94 (2005), 한국식품과학회
- 이수기, "Quercetin의 투여가 TMR의 in vitro 건물 소실률에 미치는 영향", CNU 세미나 자료 (2009)
- 조영일, "흰쥐에서 허혈성 재관류 손상에 대한 amphetamine 전처치의 신보호 효과가 heat shock protein에 의한 것인가?", Kidney Research and Clinical Practice, 23(1), pp.12~21 (2004), 대한신장학회