Biomass Production Potential of Chlorella vulgaris Under Different CO_2 Concentrations and Light Intensities
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The increasing demand of the crops(soybean and corn) for biofuel production has increased the focus of the animal nutritionists to look for alternative feeds, which are economic and environmental friendly. To identify microalgae as suitable candidate as an alternative feed, growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (0.07, 1.4, 3.0 and 5.0%) and photon densities(39.19, 72.97, 105.41, 116.22, 135.14, 175.68µmol/m^2/s) by employing a photo-bioreactor. Swine wastewater was also investigated as nutritional source to economize the biomass production. Results showed that the higher biomass production was found to be at 3.0% CO_2 compared to other CO_2 concentrations. However, no difference in biomass production was found at 105.41μmol/m^2/s and above photon densities with 12 h of photoperiodicity. It was observed that C. vulgaris could easily grow in 200 times diluted swine wastewater and growth was found to be similar with that of artificial medium. Provided the conducive conditions for optimal growth, it has also the potentiality of depleting ammonia nitrogen(NH_4-N) and orthophosphate(PO_4^3－-P) completely from the wastewater after 3~4 days of cultivation. Thus, growing C. vulgaris would not only solve the problem of animal feed, but also help in biological CO_2 mitigation and wastewater treatment.
Abstract
The increasing demand of the crops(soybean and corn) for biofuel production has increased the focus of the animal nutritionists to look for alternative feeds, which are economic and environmental friendly. To identify microalgae as suitable candidate as an alternative feed, growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (0.07, 1.4, 3.0 and 5.0%) and photon densities(39.19, 72.97, 105.41, 116.22, 135.14, 175.68µmol/m^2/s) by employing a photo-bioreactor. Swine wastewater was also investigated as nutritional source to economize the biomass production. Results showed that the higher biomass production was found to be at 3.0% CO_2 compared to other CO_2 concentrations. However, no difference in biomass production was found at 105.41μmol/m^2/s and above photon densities with 12 h of photoperiodicity. It was observed that C. vulgaris could easily grow in 200 times diluted swine wastewater and growth was found to be similar with that of artificial medium. Provided the conducive conditions for optimal growth, it has also the potentiality of depleting ammonia nitrogen(NH_4-N) and orthophosphate(PO_4^3－-P) completely from the wastewater after 3~4 days of cultivation. Thus, growing C. vulgaris would not only solve the problem of animal feed, but also help in biological CO_2 mitigation and wastewater treatment.
참고문헌 (27)
- Carvalho, A. P, "Micro algal reactors: a review of enclosed system designs and performances", Biotechnol. Prog, 22, pp.1490~1506 (2006)
- Chae, S. R, "Single cell protein production of Euglena gracilis and carbon dioxide fixation in an innovative photo-bioreactor", BIORESOURCE TECHNOLOGY, 97, pp.322~329 (2006), ELSEVIER SCI LTD
- Cheng, L, "Carbon dioxide removal from air by microalgae cultured in a membrane- photobioreactor", Sep. Purif. Technol, 50, pp.324~329 (2006)
- Chinnasamy, S, "Biomass production potential of a wastewater alga Chlorella vulgaris ARC 1 under elevated levels of CO2 and temperature", Int. J. Mol. Sci, 10, pp.518~532 (2009)
- Chiu, S. Y, "Reduction of CO2 by a high-density culture of Chlorella sp. in a semi-continuous photobioreactor", Bioresource Technol, 99, pp.3389~3396 (2008)
- de la Noue, J, "Algae and waste water", J. Appl. Phycol, 4, pp.247~254 (1992)
- De Morais, M. G, "Bio fixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor", J. Biotechnol, 129, pp.39~445 (2007)
- Gonzalez, L. E, "Efficiency of ammonia and phosphorus removal from a Colombian agro industrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus", Bioresource Technol, 60, pp.259262~ (1997)
- MijeongLeeJeong, "Carbon Dioxide Mitigation by Microalgal Photosynthesis", Bulletin of the Korean Chemical Society, 24(12), pp.1763~1766 (2003), 대한화학회
- Jin, H. F, "Influence of nitrate feeding on carbon dioxide fixation by microalgae", JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC, 2006, pp.41, 2813~2824[DOI]
- 강민숙, "기능성 생물 소재로서의 클로렐라", KSBB Journal, 19(1), pp.1~11 (2004), 한국생물공학회
- Kayombo, S, "Effect of substrate concentration on the growth of heterotrophic bacteria and algae in secondary facultative ponds", Water Res, 37, pp.2937~2943 (2003)
- Keffer, J. E, "Use of Chlorella vulgaris for CO2 mitigation in a photobioreactor", J. Ind. Microbiol. Biotechnol, 29, pp.275~280 (2002)
- Khatun, M, "Chlorella and Scenedesmus: Isolation, identification and mass cultivation for feeding cattle. Bangladesh Livestock Research Institute A report on the use of algae as potential feed supplements for cattle", pp.1~8 (1994)
- Kodama, M, "A new species of highly CO2-tolerant fast growing marine microalga suitable for high density culture", J. Mar. Biotechnol, 1, pp.21~25 (1993)
- 이진석, "Review of Advances in Biological CO2 Mitigation Technology", Biotechnology and Bioprocess Engineering, 8(6), pp.354~359 (2003), 한국생물공학회
- Mallick,N, "Biotechnological potential of immobilized algae for wastewater N, P and metal removal: a review", Biometals, 15, pp.377~390 (2002)
- Martinez. M. E, "Nitrogen and phosphorus removal from urban wastewater by the microalga Scenedesmus obliquus", Bioresource Technol, 73, pp.263~272 (2000)
- Mulbry, W, "Recycling of manure nutrients: use of algal biomass from dairy manure treatment as a slow release fertilizer", Bioresource Technol, 96, pp.451~458 (2005)
- Olguin,E.J, "Phycoremediation: key issues for cost-effective nutrient removal processes", Biotechnol. Adv, 22, pp.81~91 (2003)
- Phang,S.M, "Role of algae in livestock-fish integrated farming system", FAO, 1992, 49~56
- Preston, T. R, "Productive and sustainable livestock production: New opportunities using tropical feed resources", International Conference on Increasing Livestock Production through Utilization of Local Resources, pp.23~34 (1993)
- Shi, J, "Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study", J. Appl. Phycol, 19, pp.417~423 (2007)
- Sung, K. D, "CO2 fixation by Chlorella sp. KR-1 and its cultural characteristics", Bioresource Technol, 68, pp.269~273 (1999)
- Takagi, M, "Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella Cells", J. Biosci. Bioeng, 101, pp.223~226 (2006)
- Travieso, L, "Batch mixed culture of Chlorella vulgaris using settled and diluted piggery waste", Ecol. Engineering, 28, pp.158~165 (2006)
- Travieso, L, "Experiments on immobilization of microalgae for nutrient removal in wastewater treatments", Bioresource Technol, 5, pp.181~186 (1996)