Genetic diversity of Halla horses using microsatellite markers
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초록
Background: Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people’s weighted interest toward Jeju horses. Method: Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion: In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of Hobs was 0.749 with a range from 0.612(HMS1) to 0. 857(ASB2). Also, it was found that Hexp and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of Hexp was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was 5.90 × 10−20 when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were 4.08 × 10−15 and 3.56 × 10−8, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.
Abstract
Background: Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people’s weighted interest toward Jeju horses. Method: Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion: In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of Hobs was 0.749 with a range from 0.612(HMS1) to 0. 857(ASB2). Also, it was found that Hexp and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of Hexp was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was 5.90 × 10−20 when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were 4.08 × 10−15 and 3.56 × 10−8, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.
참고문헌 (17)
- Ayres KL, "API-CALC 1.0: computer program for calculating the average probability of identity allowing for substructure, inbreeding and the presence of close relatives", Mol Ecol Notes, 4, pp.315~318 (2004)
- Azor PJ, "Genetic characterization of the Spanish Trotter horse breed using microsatellite markers", Genet Mol Biol, 30, pp.37~42 (2007)
- Botstein D, "Construction of a genetic linkage map in man using restriction fragment length polymorphisms", Am J Hum Genet, 32, pp.314~331 (1980)
- Can˜on J, "The genetic structure of Spanish Celtic horse breeds inferred from microsatellite data", Anim Genet, 31, pp.39~48 (2000)
- 조병욱, "초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정", 생명과학회지, 17(10), pp.1441~1446 (2007), 한국생명과학회
- 조길재, "Microsatellite DNA를 이용한 말 집단의 유전적 특성 및 유연 관계", 생명과학회지, 17(5), pp.699~705 (2007), 한국생명과학회
- 조길재, "Microsatellite DNA형 분석을 이용한 더러브렛 말의 친자감정", 한국동물자원과학회지, 46(2), pp.129~136 (2004), 한국동물자원과학회
- Choi SK, "Genetic characterization and polymorphisms for parentage testing of the Jeju horse using 20 microsatellite loci", J Vet Med Sci, 70, pp.1111~1115 (2008)
- Giacomoni EH, "Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers", Genet Mol Res, 18, pp.261~270 (2008)
- Jeju Special Self-Governing Province., "A study on the appropriate utilization of Jeju crossbred horse", ~ (2013)
- Lee SY, "A Case of Parentage Testing for Thoroughbred Horse by Microsatellite DNA Typing", Korean J Forensic Sci, 7, pp.70~75 (2006)
- Marletta D, "Analysis of genetic diversity and the determination of relationships among western Mediterranean horse breeds using microsatellite markers", J Anim Breed Genet, 123, pp.315~325 (2006)
- Marshall TC, "Statistical confidence for likelihood-based paternity inference in natural populations", Mol Ecol, 7, pp.639~655 (1998)
- 2014 Actual condition survey of horse industry, "Ministry of Agriculture, Food and Rural Affairs", ~ (2015)
- Park SDE, "The excel microsatellite toolkit (version 3.1)", ~ (2001)
- Thirstrup JP, "Genetic analysis, breed assignment and conservation priorities of three native Danish horse breeds", Anim Genet, 39, pp.496~505 (2008)
- Van de Goor LHP, "A proposal for standardization in forensic equine DNA typing: allele nomenclature for 17 equine-specific STR loci", Anim Genet, 41, pp.122~127 (2009)