Human Neuroblastoma Cell Line BE2에 대한 健脾補腎抗癌湯의 세포고사 기전 연구
Anti-cancer Study of The Apoptotic Mechanism of Gunbibosinhangam-Tang on Human Neuroblastoma Cell Line BE2
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Abstract
Objective: In order to investigate cell death mechanism by Gunbibosinhangam-Tang(G.B.H) in cancer cells, the activities of apoptosis signaling pathway were tested in human neuroblastoma cell line BE2.Methods: Viability of BE2 cells was markedly decreased by treatment of the water extract of G.B.H in a dose-dependent manner. G.B.H-induced cell death was confirmed as apoptosis characterized by chromatin condensation. We tested whether the water extract of G.B.H effects the anti-apoptotic proteins such as Bcl-XL.Results: Bcl-XL was uneffected by the addition of the water extract of G.B.H. in a time-dependent manner. Cleavage of PARP(poly-ADP-ribose polymerase) by activation of caspase-8 protease was also observed in BE2 cells by the treatment of the water extract of G.B.H.Conclusion: These results suggest that the water extract of G.B.H exerts anti-cancer effects on human neuroblastoma BE2 cells by inducing the apoptotic death via activation of intrinsic caspase cascades.
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