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논문KCI 등재

四物湯 加味方이 黑色腫 細胞枯死에 미치는 效果

Effect of Samultanggamibang on Apoptosis of Melanoma cells

박은정|이해자|장성진
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2006.04|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제20권 제1호|pp. 257-272

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Abstract

Objective:In this study, the ability of Oriental medicine Samultanggamibang (SMTG) to induce apoptosis was investigated in B16F10 melanoma cells. Methods:Tetrazolium-based colorimetric assay was performed for cytotoxicity test. Several new assays for the basis of biochemical events associated with apoptosis such as DNA fragmentation by a flow cytometry, caspase-3 activation and PARP cleavage by Western blotting should be carried out potentially useful for the basis of biochemical events associated with apoptosis such as a flow cytometry and caspase-3 activation. Results:(1) The number of B16F10 melanoma cells was less than 30 % after exposure to 1 mg/ml SMTG for 48 h. SMTG increased cytotoxicity of B16F10 melanoma cells in a dose- and time-dependent manner. (2) The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 21 % at 24 h and 25 % at 48 h after treatment with 1 mg/ml SMTG. (3) SMTG-induced apoptosis was accompanied by the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymerase. (4) SMTG induces the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymerase and eventually leads to apoptosis through c-Jun NH2 -terminal protein kinase (JNK)-dependent manner in B16F10 melanoma cells. Conclusions:SMTG had a strong cytotoxic effect on B16F10 melanoma cells.

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