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참전복(Haliotis discus hannai) Myostatin 유전자의 cDNA 동정 및 발현 분석

Molecular Cloning and Expression Pattern of Abalone (Haliotis discus hannai) Myostatin cDNA

조미진|오미영|박흠기|김정환|진형주|이상범
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2009.04|한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)|제42권 제2호|pp. 139-145

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참고문헌

주제분류수산학

초록

Abstract

We cloned and sequenced the open reading frame (ORF) cDNA encoding myostatin from the muscle of abalone (Haliotis discus hannai). The ORF cDNA of the abalone myostatin is 1134 bp and encoded 377 amino acid residues that were 60-96% homologous with the amino acids of other organism myostatins. In addition, the ORF contained a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues in the C-terminus. Semi-quantitative RT-PCR revealed the presence of myostatin mRNA in various tissues. The strongest expression was observed in the mantle of female abalone, and the gills and heart of male abalone.

참고문헌 (21)

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