Effects of Bacillus-based probiotics on growth performance, nutrient digestibility, and intestinal health of weaned pigs
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Bacillus is characterized by the formation of spores in harsh environments, which makes it suitable for use as a probiotic for feed because of thermostability and high survival rate, even under long-term storage. This study was conducted to investigate the effects of Bacillus- based probiotics on growth performance, nutrient digestibility, intestinal morphology, immune response, and intestinal microbiota of weaned pigs. A total of 40 weaned pigs (7.01 ± 0.86 kg body weight [BW]; 28 d old) were randomly assigned to two treatments (4 pigs/ pen; 5 replicates/treatment) in a randomized complete block design (block = BW and sex). The dietary treatment was either a typical nursery diet based on corn and soybean meal (CON) or CON supplemented with 0.01% probiotics containing a mixture of Bacillus subtilis and Bacillus licheniformis (PRO). Fecal samples were collected daily by rectal palpation for the last 3 days after a 4-day adaptation. Blood, ileal digesta, and intestinal tissue samples were collected from one pig in each pen at the respective time points. The PRO group did not affect the feed efficiency, but the average daily gain was significantly improved (p < 0.05). The PRO group showed a trend of improved crude protein digestibility (p < 0.10). The serum transforming growth factor-β1 level tended to be higher (p < 0.10) in the PRO group on days 7 and 14. There was no difference in phylum level of the intestinal microbiota, but there were differences in genus composition and proportions. However, β-diversity analysis showed no statistical differences between the CON and the PRO groups. Taken together, Bacillus-based probiotics had beneficial effects on the growth performance, immune system, and intestinal microbiota of weaned pigs, suggesting that Bacillus can be utilized as a functional probiotic for weaned pigs.
Abstract
Bacillus is characterized by the formation of spores in harsh environments, which makes it suitable for use as a probiotic for feed because of thermostability and high survival rate, even under long-term storage. This study was conducted to investigate the effects of Bacillus- based probiotics on growth performance, nutrient digestibility, intestinal morphology, immune response, and intestinal microbiota of weaned pigs. A total of 40 weaned pigs (7.01 ± 0.86 kg body weight [BW]; 28 d old) were randomly assigned to two treatments (4 pigs/ pen; 5 replicates/treatment) in a randomized complete block design (block = BW and sex). The dietary treatment was either a typical nursery diet based on corn and soybean meal (CON) or CON supplemented with 0.01% probiotics containing a mixture of Bacillus subtilis and Bacillus licheniformis (PRO). Fecal samples were collected daily by rectal palpation for the last 3 days after a 4-day adaptation. Blood, ileal digesta, and intestinal tissue samples were collected from one pig in each pen at the respective time points. The PRO group did not affect the feed efficiency, but the average daily gain was significantly improved (p < 0.05). The PRO group showed a trend of improved crude protein digestibility (p < 0.10). The serum transforming growth factor-β1 level tended to be higher (p < 0.10) in the PRO group on days 7 and 14. There was no difference in phylum level of the intestinal microbiota, but there were differences in genus composition and proportions. However, β-diversity analysis showed no statistical differences between the CON and the PRO groups. Taken together, Bacillus-based probiotics had beneficial effects on the growth performance, immune system, and intestinal microbiota of weaned pigs, suggesting that Bacillus can be utilized as a functional probiotic for weaned pigs.
참고문헌 (58)
- Jean-Paul Lallès, "Nutritional management of gut health in pigs around weaning", Proceedings of the Nutrition Society, 66(2), pp.260~268 (2007), Cambridge University Press (CUP)[DOI]
- John R. Pluske, "Factors influencing the structure and function of the small intestine in the weaned pig: a review", Livestock Production Science, 51(1-3), pp.215~236 (1997), Elsevier BV[DOI]
- J.C. Kim, "Nutrition and pathology of weaner pigs: Nutritional strategies to support barrier function in the gastrointestinal tract", Animal Feed Science and Technology, 173(1-2), pp.3~16 (2012), Elsevier BV[DOI]
- D. O. Krause, "Response of nursery pigs to a synbiotic preparation of starch and an anti-Escherichia coli K88 probiotic", Applied and Environmental Microbiology, 76(24), pp.8192~8200 (2010), American Society for Microbiology[DOI]
- Z.C. Dlamini, "Effects of probiotics on growth performance, blood parameters, and antibody stimulation in piglets", South African Journal of Animal Science, 47(6), pp.765~775 (2017), African Journals Online (AJOL)[DOI]
- J. E. Pettigrew, "Reduced Use of Antibiotic Growth Promoters in Diets Fed to Weanling Pigs: Dietary Tools, Part 1", Animal Biotechnology, 17(2), pp.207~215 (2006), Informa UK Limited[DOI]
- Hans H. Stein, "Reduced Use of Antibiotic Growth Promoters in Diets Fed to Weanling Pigs: Dietary Tools, Part 2", Animal Biotechnology, 17(2), pp.217~231 (2006), Informa UK Limited[DOI]
- J. M. Heo, "Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds", Journal of Animal Physiology and Animal Nutrition, 97(2), pp.207~237 (2013), Wiley[DOI]
- Jun Soeng Lee, "Effects of microencapsulated organic acids on growth performance, nutrient digestibility, fecal microbial counts, and blood profiles in weaning pigs", 한국축산학회지, 63(1), pp.104~113 (2021), 한국축산학회[DOI]
- C.F.M. de Lange, "Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigs", Livestock Science, 134(1-3), pp.124~134 (2010), Elsevier BV[DOI]
- Shengfa F. Liao, "Using probiotics to improve swine gut health and nutrient utilization", Animal Nutrition, 3(4), pp.331~343 (2017), Elsevier BV[DOI]
- 민예진, "Effects of dietary mixture of protease and probiotics on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs", 한국축산학회지, 61(5), pp.272~277 (2019), 한국축산학회[DOI]
- Joowon Kang, "Effects of dietary inactivated probiotics on growth performance and immune responses of weaned pigs", 한국축산학회지, 63(3), pp.520~530 (2021), 한국축산학회[DOI]
- He Y, "Bacillus subtilis: a potential growth promoter in weaned pigs in comparison to carbadox", J Anim Sci, 98, pp.skaa290~ (2020)[DOI]
- S. Mingmongkolchai, "Bacillusprobiotics: an alternative to antibiotics for livestock production", Journal of Applied Microbiology, 124(6), pp.1334~1346 (2018), Wiley[DOI]
- Yuanliang Hu, "Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets", Animal Bioscience, 27(8), pp.1131~1140 (2014), 아세아·태평양축산학회[DOI]
- National Research Council, "Nutrient requirements of swine" (2012), National Academy Press
- Jeong Jae Lee, "Dietary Yeast Cell Wall Improves Growth Performance and Prevents of Diarrhea of Weaned Pigs by Enhancing Gut Health and Anti-Inflammatory Immune Responses", Animals, 11(8), pp.2269~ (2021), MDPI AG[DOI]
- 박상우, "Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs", 한국축산학회지, 62(1), pp.21~30 (2020), 한국축산학회[DOI]
- Association of Official Analytical Chemists International, "Official methods of analysis of AOAC International" (2005), AOAC International
- H. H. Stein, "Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application", Journal of Animal Science, 85(1), pp.172~180 (2007), Oxford University Press (OUP)[DOI]
- Hyunjin Kyoung, "Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs", Animals, 11(2), pp.504~ (2021), MDPI AG[DOI]
- Zhang Song, "Effects of dietary probiotic, liquid feed and nutritional concentration on the growth performance, nutrient digestibility and fecal score of weaning piglets", Animal Bioscience, 33(10), pp.1617~1623 (2020), 아세아·태평양축산학회[DOI]
- Y. Wang, "The effect of probiotic BioPlus 2B® on growth performance, dry matter and nitrogen digestibility and slurry noxious gas emission in growing pigs", Livestock Science, 120(1-2), pp.35~42 (2009), Elsevier BV[DOI]
- Jens Noesgaard Jørgensen, "Effects of a Bacillus -based probiotic and dietary energy content on the performance and nutrient digestibility of wean to finish pigs", Animal Feed Science and Technology, 221, pp.54~61 (2016), Elsevier BV[DOI]
- Diana Luise, "Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics", Journal of Animal Science and Biotechnology, 10(1), pp.74~ (2019), Springer Science and Business Media LLC[DOI]
- Ruixia Lan, "Effects of probiotic supplementation in different nutrient density diets on growth performance, nutrient digestibility, blood profiles, fecal microflora and noxious gas emission in weaning pig", Journal of the Science of Food and Agriculture, 97(4), pp.1335~1341 (2017), Wiley[DOI]
- Huan Wang, "Influence of Bacillus subtilis GCB-13-001 on growth performance, nutrient digestibility, blood characteristics, faecal microbiota and faecal score in weanling pigs", Journal of Animal Physiology and Animal Nutrition, 103(6), pp.1919~1925 (2019), Wiley[DOI]
- I.H. Kim, "The Benefits of Using Bacillus as a Probiotic", Journal of Animal and Veterinary Advances, 11(18), pp.3457~3462 (2012), Medwell Publications[DOI]
- Pipat Piewngam, "Pathogen elimination by probiotic Bacillus via signalling interference", Nature, 562(7728), pp.532~537 (2018), Springer Science and Business Media LLC[DOI]
- F G Priest, "Extracellular enzyme synthesis in the genus Bacillus", Bacteriological Reviews, 41(3), pp.711~753 (1977), American Society for Microbiology[DOI]
- Das G, "Isolation, purification & mass production of protease enzyme from Bacillus subtilis", Int Res J Microbiol, 1, pp.26~31 (2010)
- D.J. HAMPSON, "Alterations in piglet small intestinal structure at weaning", Research in Veterinary Science, 40(1), pp.32~40 (1986), Elsevier BV[DOI]
- Huansheng Yang, "Effects of weaning on intestinal crypt epithelial cells in piglets", Scientific Reports, 6(1), pp.36939~ (2016), Springer Science and Business Media LLC[DOI]
- L. Cai, "Effects of a multi-strain Bacillus species–based direct-fed microbial on growth performance, nutrient digestibility, blood profile, and gut health in nursery pigs fed corn–soybean meal–based diets", Journal of Animal Science, 93(9), pp.4336~4342 (2015), Oxford University Press (OUP)[DOI]
- Y. Liu, "Dietary plant extracts alleviate diarrhea and alter immune responses of weaned pigs experimentally infected with a pathogenic Escherichia coli", Journal of Animal Science, 91(11), pp.5294~5306 (2013), Oxford University Press (OUP)[DOI]
- Yue Li, "Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets", Journal of Animal Science and Biotechnology, 9(1), pp.22~ (2018), Springer Science and Business Media LLC[DOI]
- M. C. Walsh, "Effects of water and diet acidification with and without antibiotics on weanling pig growth and microbial shedding", Journal of Animal Science, 85(7), pp.1799~1808 (2007), Oxford University Press (OUP)[DOI]
- S. K. Bhandari, "Evaluation of alternatives to antibiotics using an Escherichia coli K88+ model of piglet diarrhea: Effects on gut microbial ecology", Journal of Animal Science, 86(4), pp.836~847 (2008), Oxford University Press (OUP)[DOI]
- J.Y. Choi, "Evaluation of multi-microbe probiotics prepared by submerged liquid or solid substrate fermentation and antibiotics in weaning pigs", Livestock Science, 138(1-3), pp.144~151 (2011), Elsevier BV[DOI]
- Deng B, "Effects of Bacillus subtilis on growth performance, serum parameters, digestive enzyme, intestinal morphology, and colonic microbiota in piglets", AMB Express, 10, pp.212~ (2020)[DOI]
- Wei Zhang, "Oral administration of a select mixture of Bacillus probiotics affects the gut microbiota and goblet cell function following Escherichia coli challenge in newly weaned pigs of genotype MUC4 that are supposed to be enterotoxigenic E. coli F4ab", ac receptor negative, 2017, pp.83(3), e02747~16[DOI]
- Todd R. Klaenhammer, "The impact of probiotics and prebiotics on the immune system", Nature Reviews Immunology, 12(10), pp.728~734 (2012), Springer Science and Business Media LLC[DOI]
- Zeuthen LH, "Epithelial cells prime the immune response to an array of gut-derived commensals towards a tolerogenic phenotype through distinct actions of thymic stromal lymphopoietin and transforming growth factor-β", Immunology, 123, pp.197~208 (2008)
- Dong Zhou, "Oral administration of a select mixture of Bacillus probiotics generates Tr1 cells in weaned F4ab", acR− pigs challenged with an F4+ ETEC, EPEC strain, pp.Veterinary Research, 2015[DOI]
- Ewa Laskowska, "Effect of multi-microbial probiotic formulation bokashi on pro- and anti-inflammatory cytokines profile in the serum, colostrum and milk of sows, and in a culture of polymorphonuclear cells isolated from colostrum", Probiotics and Antimicrobial Proteins, 11(1), pp.220~232 (2019), Springer Science and Business Media LLC[DOI]
- McDermott MF, "TNF and TNFR biology in health and disease", Cell Mol Biol, 47, pp.619~635 (2001)
- Anna Zganiacz, "TNF-α is a critical negative regulator of type 1 immune activation during intracellular bacterial infection", Journal of Clinical Investigation, 113(3), pp.401~413 (2004), American Society for Clinical Investigation[DOI]
- Kaywan Izadpanah, "Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis", Journal of Neuroimmunology, 276(1-2), pp.104~111 (2014), Elsevier BV[DOI]
- R. X. Lan, "Effects of multistrain probiotics on growth performance, nutrient digestibility, blood profiles, faecal microbial shedding, faecal score and noxious gas emission in weaning pigs", Journal of Animal Physiology and Animal Nutrition, 100(6), pp.1130~1138 (2016), Wiley[DOI]
- Núria Mach, "Early-life establishment of the swine gut microbiome and impact on host phenotypes", Environmental Microbiology Reports, 7(3), pp.554~569 (2015), Wiley[DOI]
- Clare H. Gaukroger, "Changes in Faecal Microbiota Profiles Associated With Performance and Birthweight of Piglets", Frontiers in Microbiology, 11, pp.917~ (2020), Frontiers Media SA[DOI]
- J.M. Fouhse, "The role of gut microbiota in the health and disease of pigs", Animal Frontiers, 6(3), pp.30~36 (2016), Oxford University Press (OUP)[DOI]
- Qingsen Shang, "Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae", Food & Function, 7(7), pp.3224~3232 (2016), Royal Society of Chemistry (RSC)[DOI]
- Robert Pieper, "Ecophysiology of the Developing Total Bacterial and Lactobacillus Communities in the Terminal Small Intestine of Weaning Piglets", Microbial Ecology, 56(3), pp.474~483 (2008), Springer Science and Business Media LLC[DOI]
- Petia Kovatcheva-Datchary, "Dietary Fiber-Induced Improvement in Glucose Metabolism Is Associated with Increased Abundance of Prevotella", Cell Metabolism, 22(6), pp.971~982 (2015), Elsevier BV[DOI]
- H. Ding, "Dietary supplementation with Bacillus subtilis DSM 32315 alters the intestinal microbiota and metabolites in weaned piglets", Journal of Applied Microbiology, 130(1), pp.217~232 (2021), Wiley[DOI]
- K. Cui, "Effects of dietary supplementation with Bacillus subtilis and yeast culture on growth performance, nutrient digestibility, serum indices and faeces microbiota of weaned piglets", Journal of Animal and Feed Sciences, 28(4), pp.328~336 (2019), The Kielanowski Institute of Animal Physiology and Nutrition, PAS[DOI]
