大補陰丸이 Streptozotocin으로 유도된 白鼠의 당뇨병 및 당뇨병 합병증에 미치는 영향
Effects of Daeboeum-hwan Aqueous Extracts on Streptozotocin-induced Rat Diabetes and Related Complications
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Objectives : The object of this study was to investigate the effects of Daeboeum-hwan (DBEH) aqueous extracts on Streptozotocin (STZ)-induced rat diabetes and related complications. Methods : SD rats were divided into 6 groups (n=8), 50 mM citrate buffer and vehicle (distilled water 5 ㎖/㎏) administered group (Intact control), STZ treatment (60 ㎎/㎏, single intraperitoneally administered) and vehicle administered group (STZ control), STZ treatment and silymarin 100 ㎎/㎏ administered group (Reference), STZ treatment and DBEH extracts 50, 100 and 200 ㎎/㎏ administered groups. DBEH extracts were orally administered once a day for 28 days from 3 weeks after STZ treatment. The results were compared with silymarin 100 ㎎/㎏. Results : Decreases on the body weights, increase of kidney and liver weights, blood glucose, BUN, creatinine, AST, ALT, LDL, triglyceride and total cholesterol levels with decreases of HDL levels, increases of pancreatic MDA contents and decreases of GSH contents were detected in STZ control as compared with intact control. These diabetes and related complications were inhibited by treatment of 100 and 200 ㎎/㎏ of DBEH extracts. Conclusion : DBEH extracts showed favorable effects on the STZ-induced diabetes and related complications mediated by their antioxidant effects as similar to silymarin. Because of lowest dosage (50 ㎎/㎏) of DBEH treated group did not showed any favorable effects as compared with STZ control, the effective dosages of DBEH is considered as about 100 ㎎/㎏, and the effects of DBEH extracts 200 ㎎/㎏ were showed similar effects as compared with 100 ㎎/㎏ of silymarin. It, therefore, is expected that DBEH will be showed favorable effects on the diabetes and various diabetic complications.
Abstract
Objectives : The object of this study was to investigate the effects of Daeboeum-hwan (DBEH) aqueous extracts on Streptozotocin (STZ)-induced rat diabetes and related complications. Methods : SD rats were divided into 6 groups (n=8), 50 mM citrate buffer and vehicle (distilled water 5 ㎖/㎏) administered group (Intact control), STZ treatment (60 ㎎/㎏, single intraperitoneally administered) and vehicle administered group (STZ control), STZ treatment and silymarin 100 ㎎/㎏ administered group (Reference), STZ treatment and DBEH extracts 50, 100 and 200 ㎎/㎏ administered groups. DBEH extracts were orally administered once a day for 28 days from 3 weeks after STZ treatment. The results were compared with silymarin 100 ㎎/㎏. Results : Decreases on the body weights, increase of kidney and liver weights, blood glucose, BUN, creatinine, AST, ALT, LDL, triglyceride and total cholesterol levels with decreases of HDL levels, increases of pancreatic MDA contents and decreases of GSH contents were detected in STZ control as compared with intact control. These diabetes and related complications were inhibited by treatment of 100 and 200 ㎎/㎏ of DBEH extracts. Conclusion : DBEH extracts showed favorable effects on the STZ-induced diabetes and related complications mediated by their antioxidant effects as similar to silymarin. Because of lowest dosage (50 ㎎/㎏) of DBEH treated group did not showed any favorable effects as compared with STZ control, the effective dosages of DBEH is considered as about 100 ㎎/㎏, and the effects of DBEH extracts 200 ㎎/㎏ were showed similar effects as compared with 100 ㎎/㎏ of silymarin. It, therefore, is expected that DBEH will be showed favorable effects on the diabetes and various diabetic complications.
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