山靑木이 간섬유화 진행 억제에 미치는 효과에 대한 연구
A Study of the Inhibitory Effect of Acer tegmentosum Max. on Fibrogenesis in Hepatic Stellate Cell Line T6
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Objectives : This study was performed in order to investigate the anti-fibrogenic effect of Acer tegmentosum Maxim. on rat hepatic stellate cell line T6. Materials and Methods : Hepatic stellate Cells (T6) were treated with various concentrations of distilled water Acer tegmentosum Maxim. extract for 24, 48, 72 hours. After the treatment, cell viability, proliferation, procollagen levels, mRNA of ASMA, MMP-2, collagen type 1a2 and IL-6 production were measured using MTT assay, BrdU assay, RT-PCR, procollagen type 1 C-peptide EIA kit and murine IL-6 ELISA development kit. Results : Cell viability of HSC-T6 decreased significantly in both 24 hours and 48 hours groups in a dose-dependant manner. Proliferation of HSC also decreased in the same way. In the RT-PCR, mRNA expression of collagen type 1a2 and ASMA decreased in the groups which were treated with Acer tegmentosum Maxim. for 24 hours. The production of procollagen tended to decrease in a dose-dependant manner in the 24 hours treated group. IL-6 production increased under Acer tegmentosum treatment in a dose-dependant manner in both 24 and 48 hours groups. Conclusion : These results show the possibility that Acer tegmentosum Maxim. can be an effective remedy for liver fibrosis and liver cirrhosis.
Abstract
Objectives : This study was performed in order to investigate the anti-fibrogenic effect of Acer tegmentosum Maxim. on rat hepatic stellate cell line T6. Materials and Methods : Hepatic stellate Cells (T6) were treated with various concentrations of distilled water Acer tegmentosum Maxim. extract for 24, 48, 72 hours. After the treatment, cell viability, proliferation, procollagen levels, mRNA of ASMA, MMP-2, collagen type 1a2 and IL-6 production were measured using MTT assay, BrdU assay, RT-PCR, procollagen type 1 C-peptide EIA kit and murine IL-6 ELISA development kit. Results : Cell viability of HSC-T6 decreased significantly in both 24 hours and 48 hours groups in a dose-dependant manner. Proliferation of HSC also decreased in the same way. In the RT-PCR, mRNA expression of collagen type 1a2 and ASMA decreased in the groups which were treated with Acer tegmentosum Maxim. for 24 hours. The production of procollagen tended to decrease in a dose-dependant manner in the 24 hours treated group. IL-6 production increased under Acer tegmentosum treatment in a dose-dependant manner in both 24 and 48 hours groups. Conclusion : These results show the possibility that Acer tegmentosum Maxim. can be an effective remedy for liver fibrosis and liver cirrhosis.
참고문헌 (25)
- 통계청, "사망원인통계", ~ (2008)
- Jun-Sung Lee, "The Role of Activated Hepatic Stellate Cells in Liver Fibrosis, Portal Hypertension and Cancer Angiogenesis", The Korean Journal of Hepatology, 13, pp.309~319 (2007)
- 최은경, "丹蔘이 간성상세포의 섬유화 억제에 미치는 영향", 대한한방내과학회지, 29(2), pp.299~310 (2008), 대한한방내과학회
- 김성아, "茵蔯이 간성상세포의 섬유화 억제에 미치는 영향", 대한한방내과학회지, 29(1), pp.177~188 (2008), 대한한방내과학회
- 김세훈, "鬱金이 간성상세포의 섬유화 억제에 미치는 영향", 대한한방내과학회지, 30(2), pp.306~316 (2009), 대한한방내과학회
- 이홍일, "茵蔯清肝汤이 간성상세포의 섬유화 억제에 미치는 영향", 대한한방내과학회지, 30(1), pp.74~84 (2009), 대한한방내과학회
- 홍보경, "산겨릅나무 줄기추출물의 생리활성 및 유효성분 분리", 한국약용작물학회지, 15(4), pp.296~303 (2007), 한국약용작물학회
- 신인철, "산겨릅나무 추출물의 이화학적 특성과 암세포 성장 억제 효과", Journal of the Korean Society for Applied Biological Chemistry, 49(4), pp.322~327 (2006), 한국응용생명화학회
- 김성훈, "산겨릅나무에서 분리한 salidroside의 간보호 효과", 동의생리병리학회지, 22(6), pp.1525~1531 (2008), 대한동의병리학회
- 권하나, "산겨릅나무 추출물의 항산화 및 간 기능 보호효과", 한국식품영양과학회지, 37(11), pp.1389~1394 (2008), 한국식품영양과학회
- Friedman SL, "Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury", J Biol Chem., 275, pp.2247~2250 (2000)
- Young-Han Paik, "Fibrogenesis and antifibrotic therapy", The Korean Journal of Hepatology, 25(2s), pp.7~13 (2008)
- Hasegawa T, "Plasma transforming growth factor -beta1 level and efficacy of alpha-tocopherol in patients with non-alcoholic steatohepatitis: a pilot study", Aliment Pharmacol Ther., 15, pp.1667~1672 (2001)
- Lindor KD, "Ursodeoxycholic acid for treatment of nonalcoholic steatohepatitis: results of a randomized trial", Hepatology, 39, pp.770~778 (2004)
- Yokohama S, "Therapeutic efficacy of an angiotensin", ~
- 안덕균, "한국본초도감", pp.523~ (1998), 교학사
- 김일훈, "神藥", pp.78~79 (1986), 인산동천
- 허종문, "산겨릅나무 줄기에서 페놀성 글루코사이드의 분리", Journal of the Korean Society for Applied Biological Chemistry, 49(2), pp.149~152 (2006), 한국응용생명화학회
- 허종문, "산겨릅나무 줄기에서 이소프렌계 화합물의 분리", 생약학회지, 38(1), pp.67~70 (2007), 한국생약학회
- 김성훈, "흰쥐에서 Cyclosporine 유도가 산겨릅나무에서 분리한 salidroside의 신독성 경감기전에 관한 효과", 동의생리병리학회지, 23(1), pp.166~173 (2009), 대한동의병리학회
- Xiao-Dong Peng, "Correlation between anti-fibrotic effect of baicalin and serum cytokines in rat hepatic fibrosis", World J Gastroenterol, 15(37), pp.4720~4725 (2009)
- Nathalie Franchimont, "Interleukin-6 and Its Soluble Receptor Cause a Marked Induction of Collagenase 3 Expression in Rat Osteoblast Cultures", J Biol Chem., 272(18), pp.12144~12150 (1997)
- Kenichiro Kusano, "Regulation of Matrix Metalloproteinases (MMP-2, -3, -9, and -13) by Interleukin-1 and Interleukin -6 in Mouse Calvaria: Association of MMP Induction with Bone Resorption", Endocrinology, 139(3), pp.1338~1345 (1998)
- Solis-Herruzo JA, "Interleukin-6 increases rat metalloproteinase-13 gene expression through stimulation of activator protein 1 transcription factor in cultured fibroblasts", J Biol Chem., 274(43), pp.30919~30926 (1999)
- J. A. Solis Herruzo, "IL-6 and extracellular matrix remodelling", Rev Esp Enferm Dig, 97(8), pp.575~595 (2005)