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감성돔(Acanthopagrus schlegelii) 치어의 포르말린에 대한 내성및 조직학적 반응

Tolerance and Histological Responses to Formalin of Black Seabream Acanthopagrus schlegelii Juveniles

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초록

Black seabream juveniles Acanthopagrus schlegelii held at 20°C were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24,48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis,epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization,deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.

Abstract

Black seabream juveniles Acanthopagrus schlegelii held at 20°C were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24,48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis,epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization,deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.

참고문헌 (34)

  1. Agamy E, "Histopathological liver alterations in juvenile rabbit fish (Siganus canaliculatus) exposed to light Arabian crude oil, dispersed oil and dispersant", Ecotoxicol Environ Saf, 75, pp.171~179 (2012)[DOI]
  2. Allison R, "Some new results in the treatment of ponds to control some external parasites of fish", Prog Fish-Cult, 19, pp.58~63 (1957)
  3. Bills TD, "Investigation in fish control. Formalin: Its Toxicity to Non Target Aquatic Organisms, Persistence and Counteraction 73", US Department of the Interior, Fish and Wildlife Service, 1~7 (1977)
  4. Couch JA, "Spongiosis hepatis: chemical induction, pathogenesis and possible neoplastic fate in a teleost fish model", Toxicol Pathol, 19, pp.237~250 (1991)
  5. Couillard CM, "Pigmented macrophage aggregates: a toxic response in fish exposed to bleachedkraft mill effluent", Environ Toxicol Chem, 15, pp.1844~1854 (1996)
  6. Cruz ER, "Tolerance level and histopathological response of milkfish (Chanos chanos) fingerlings to formalin", Aquaculture, 78, pp.135~145 (1989)
  7. Dutta HM, "Histopathological changes induced by malathion in the liver of a freshwater catfish, Heteropneustes fossilis (Bloch)", B Environ Contam Tox, 51, pp.895~900 (1993)
  8. Fajer-Ávila EJ, "Toxicity of formalin to bullseye puffer fish (Sphoeroides annulatus Jenyns, 1843) and its effectiveness to control ectoparasites", Aquaculture, 223, pp.41~50 (2003)[DOI]
  9. Hinton MJ, "Toxicity of ten commonly used chemicals to american eels", Proc Ann Conf S.E Assoc Fish Wildl Agencies, 32, pp.599~ (1978)
  10. Hinton DE, "Liver Structural Alterations Accompanying Chronic Toxicity in Fishes: Potential Biomarkers of Exposure. Biomarkers of Environmental Contamination", pp.12~68 (1990), Lewis Publishers
  11. Hughes JS, "Toxicity of some chemicals to striped bass (Roccus saxatilis)", Proc Ann Conf S.E. Game Fish Comm, 22, pp.230~234 (1969)
  12. Jung SH, "The lethal concentration (LC50) and lethal time (LT50) of formalin on flounder fingerlings, Paralichthys olivaceus", Bull Nat Fish Res Dev Inst, 54, pp.125~129 (1998)
  13. 정승희, "뱀장어에 대한 포르말린의 24시간 반수치사농도 (LC50)", 한국어병학회지, 20(2), pp.161~167 (2007), 한국어병학회
  14. Kabata Z, "Parasites and Diseases of Fish Cultured in Tropics", pp.318~ (1985), Taylor & Francis
  15. Lauren BJ, "Cytotoxicity phase of diethylnitrosamine- induced hepatic neoplasia in medaka", Cancer Res, 50, pp.5504~5514 (1990)
  16. Manera M, "Macrophage aggregates in gilthead sea bream fed copper, iron and zinc enriched diets", J Fish Biol, 57, pp.457~465 (2000)
  17. Marty GD, "Retrospective analysis: bile hydrocarbons and histopathology of demersal rockfish in Prince William Sound, Alaska, after the Exxon Valdez oil spill", Mar Environ Res, 56, pp.569~584 (2003)[DOI]
  18. McKim JM, "Environmental impacts on the physiological mechanisms controlling xenobiotic transfer across fish gills", Physiol Zool, 64, pp.39~67 (1991)
  19. Mitchell AJ, "Nipurfirinol (Furanace: P-7138) related lesions on channel catfish Ictalurus punctatus (Rafinesque)", J Fish Dis, 1, pp.115~121 (1978)
  20. Ottoboni MA, "The Dose Makes the Poison", pp.200~244 (1991), Van Nostrand Reinhold
  21. Pacheco, M, "Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla Anguilla L.)", Ecotoxicol Environ Saf, 53, pp.331~347 (2007)[DOI]
  22. Park IS, "Acute toxicity effects of formalin to flounder, Paralichthys olivaceus", J fish Pathol, 8, pp.57~67 (1995)
  23. Perera HACC, "Effects of short term exposure to therapeutic levels of formalin on health status of nile tilapia, Oreochromis niloticus", J Nat Sci Foundation Sri Lanka, 33, pp.239~245 (2005)
  24. Pierce KV, "Histopathology of abnormal livers and other organs of starry flounder Platichthys stellatus (Pallas) from the estuary of the Duwamish River, Seattle, Washington, U.S.A", J Fish Dis, 3, pp.81~91 (1980)
  25. Rankin J C, "Effects of pollutants on gills, In Gills", pp.207~220 (1982), Cambridge University Press
  26. Reardon IS, "Acute toxicity of formalin and copper sulfate to striped bass fingerlings held in varying salinities", Aquaculture, 87, pp.255~270 (1990)
  27. Rodrigues EL, "Liver histopathology of the fish Brachydanio rerio after acute exposure to sublethal levels of the organophosphate dimethoat 500", Rev Bras Zool, 15, pp.441~450 (1998)
  28. Smith CE, "Pathological effects in formalin treated rainbow trout (Salmo gairdneri)", J Fish Res Board Can, 29, pp.328~329 (1972)
  29. Subasinghe RP, "The use of therapeutic agents in aquaculture in Sri Lanka, In Diseases in Asian AquacultureⅠ", pp.547~553 (1992), Fish Health Section, Asian Fish Soc
  30. Tonguthai K, "The use of chemotherapeutic agents in aquaculture in Thailand, In Diseases in Asian AquacultureⅠ", pp.555~565 (1992), Fish Health Section, Asian Fish Soc
  31. Wedemeyer G, "Stress of formalin treatment in juvenile spring chinook salmon (Oncorhynchus tshawytscha) and steelhead trout (Salmo gairdneri)", J Fish Res Board Can, 31, pp.179~194 (1974)
  32. Wenderlaar Bonga SE, "Toxicants and osmoregulation in fish", Neth J Zool, 42, pp.478~493 (1992)
  33. Williams HA, "Some effects of therapeutic levels of formalin and copper sulphate on blood parameters in rainbow trout", Aquaculture, 24, pp.341~353 (1981)
  34. Wolfe JC, "A brief overview of nonneoplastic hepatic toxicity in fish", Toxicol Pathol, 33, pp.75~85 (2005)[DOI]

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