調胃升淸湯 추출액이 비만유도 쥐의 지질대사, 항산화계 및 염증반응계에 미치는 영향
Effects of Jowiseungcheung-tang Extract on the Lipid Metabolism, Anti-oxidation and Inflammatory Reflex High Fat Diet Obese Rats
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Objectives : The purpose of this study was to investigate the effects of Jowiseungcheung-tang (JWSCT) extract on the lipid metabolism, anti-oxidation and inflammatory reflex. Methods : Male Sprague-Dawley rats were fed a high fat diet for 8 weeks and were randomly divided into 4 groups (10 mice in each group): control group, 100 mg/kg JWSCT group, 200 mg/kg JWSCT group, 300 mg/kg JWSCT group. The control group was administered 100 mg/kg of water, but the other three groups were administered 100, 200, 300 mg/kg JWSCT extract for 4 weeks. After 4 weeks, we measured lipid level, thiobarbituric acid reactive substance (TBARS), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cytokines in plasma and liver. The gene expression level and the ratio of apo-B and apo-E were then investigated by way of reverse transcription -polymerase chain reaction (RT-PCR). Results : In the JWSCT group, compared with the control, free fatty acid, triglyceride, total cholesterol, LDL-cholesterol, TBARS, IL-1β, IL-6 and TNF-α decreased significantly in plasma and liver. However HDL-cholesterol, IL-10, GSH-Px, SOD and CAT increased. In the JWSCT group, compared with the control, the gene expression level and the ratio of apo-A and apo-E decreased significantly in the RT-PCR analysis. Conclusions : The extract of JWSCT has anti-obesity, anti-inflammatory and anti-oxidant effects.
Abstract
Objectives : The purpose of this study was to investigate the effects of Jowiseungcheung-tang (JWSCT) extract on the lipid metabolism, anti-oxidation and inflammatory reflex. Methods : Male Sprague-Dawley rats were fed a high fat diet for 8 weeks and were randomly divided into 4 groups (10 mice in each group): control group, 100 mg/kg JWSCT group, 200 mg/kg JWSCT group, 300 mg/kg JWSCT group. The control group was administered 100 mg/kg of water, but the other three groups were administered 100, 200, 300 mg/kg JWSCT extract for 4 weeks. After 4 weeks, we measured lipid level, thiobarbituric acid reactive substance (TBARS), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cytokines in plasma and liver. The gene expression level and the ratio of apo-B and apo-E were then investigated by way of reverse transcription -polymerase chain reaction (RT-PCR). Results : In the JWSCT group, compared with the control, free fatty acid, triglyceride, total cholesterol, LDL-cholesterol, TBARS, IL-1β, IL-6 and TNF-α decreased significantly in plasma and liver. However HDL-cholesterol, IL-10, GSH-Px, SOD and CAT increased. In the JWSCT group, compared with the control, the gene expression level and the ratio of apo-A and apo-E decreased significantly in the RT-PCR analysis. Conclusions : The extract of JWSCT has anti-obesity, anti-inflammatory and anti-oxidant effects.
참고문헌 (39)
- 서영성, "고지방 저제지방 비비만 여성에서 전염증성 사이토카인과 인슐린저항성", Diabetes and Metabolism Journal, 31(2), pp.136~143 (2007), 대한당뇨병학회
- 김종엽, "비만도에 따른 Adiponectin, Leptin, Ghrelin과 인슐린 저항성, 심혈관 위험인자의 상호 연관성", 대한내과학회지, 69(6), pp.631~641 (2005), 대한내과학회
- Ali SR, "Dietary and Genetic Obesity Promote Liver Inflammation and Tumorigenesis by Enhancing IL-6 and TNF Expression", 대한간암학회지, 10, pp.82~83 (2010)
- 김달호, "注解補注 黃帝內經 素問(上)", pp.652~ (2001), 의성당
- 진문개, "高脂血症的中醫防治", pp.9~20 (1988), 中醫古籍出版社
- 허준, "東醫寶鑑", pp.741~ (2002), 법인문화사
- 서남준, "加味太陰調胃湯 전탕액을 활용한 비만 환자에 대한 임상 연구", 동의생리병리학회지, 22(2), pp.446~452 (2008), 대한동의병리학회
- 박선민, "고지방식이로 비만을 유도한 암컷 백서에서 태음조위탕과 도담탕의 항비만 효과 및 기전", Journal of Applied Biological Chemistry, 53(1), pp.44~50 (2010), 한국응용생명화학회
- 박태용, "體減薏苡仁湯의 항비만 효과 연구", 동의생리병리학회지, 22(3), pp.642~648 (2008), 대한동의병리학회
- 송윤경, "비만처방에서 마황의 임상활용에 대한 고찰: 용량, 효과, 부작용 등의 측면에서", 대한한방비만학회지, 7(1), pp.1~7 (2007)
- 김성옥, "지방세포 3T3-L1에 인삼 사포닌 Re와 의이인 추출액 처리시 비만관련 유전자인 TNF-α, lipoprotein lipase, leptin 및 resistin 발현 조절에 미치는 영향", 생명과학회지, 17(11), pp.1523~1532 (2007), 한국생명과학회
- 김선민, "蒼朮 에탄올 추출물이 비만세포 매개 염증반응에 미치는 영향", 한방안이비인후피부과학회지, 24(1), pp.45~63 (2011), 대한한방안이비인후피부과학회
- 조세왕, "太陰調胃湯이 비만유발 흰쥐의 체중감량, 혈청지질 및 UCP1 발현에 미치는 영향", 대한한의학회지, 25(2), pp.87~97 (2004), 대한한의학회
- Lee GJ, "Effects of Taeyeumjowee -tang on the obesity of mouse and induced adipocyte 3T3-L1", Korean J Oriental Medical Pathology, 10(1), pp.88~98 (1996)
- Jang YJ, "Effect of Taeyeumjowee -tang and electroacupuncture combined-therapy on", J Korean Oriental Association for Study of Obesity, 1(1), pp.77~83 (2001)
- 2007년 국민건강통계결과, "통계청", 보건복지가족부, 56~ (2007)
- 이기주, "태음조위탕이 백서의 비만증 및 유도비만세포에 미치는 효과", 대한동의병리학회지, 10(1), pp.88~98 (1996)
- 류재면, "우울증모델 흰쥐에 대한 조위승청탕과 fluoxetine의 항우울효과 비교", 동의신경정신과학회지, 15(1), pp.27~41 (2004)
- 한진수, "조위승청탕의 위점막 손상 방어효과에 관한 연구", 사상체질의학회지, 14(1), pp.100~111 (2002)
- 류지걸, "생약 복합제재의 약효연구26: 조위승청탕의 항경련, 진통, 진경, 적출장관, 혈관, 혈압 및 호흡에 관한 영향", 생약학회지, 64, pp.12~18 (1986)
- 이귀녕, "임상병리파일", pp.148~ (2000), 의학문화사
- "비만관리를 위한 영양길잡이", pp.30~32 (2003), 한미의학
- Nielsen S, "Obesity and cardivascular disease is body structure a factor", Curr Opin Lipidol, 8(4), pp.200~204 (1997)
- Bruce AF, "Biology of disease: Free radicals and tissue injury", Lab Invest, 47, pp.412~426 (1982)
- Lioyd D, "How to avoid oxygen", Science, 286, pp.249~ (1999)
- Kesavulu MM, "Lipid peroxidation and antioxidant enzyme levels in type 2 diabetics with microvascular complications", Diabetes Metab, 26(5), pp.387~392 (2000)
- Oppenheim JJ, "Cytokines: past, present and future", Int J of Hematology, 74(1), pp.3~8 (2001)
- Weigle DS, "Appetite and the regulation of body composition", FASEB J, 8(3), pp.302~310 (1994)
- Nielsen S, "Obesity and cardiovascular disease is body structure a factor", Curr Opin Lipidol, 8(4), pp.200~204 (1997)
- Mohamed-Ali V, "Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo", J Clin Endocrinol Metab, 82, pp.4196~4200 (1997)
- Mohamed-Ali V, "Adipose tissue an as endocrine and paracrine organ", Int J Obes, 22, pp.1145~1158 (1998)
- 양정화, "조릿대 잎 추출물이 고지방식이 유도 비만마우스(C57", BL6J)의 염증성 사이토카인과 인슐린 저항성에 미치는 영향, 전남대학교 교육대학원, pp.영양교육, 2008
- Jansson JO, "On the site and mechanism of action of the anti-obesity effects of interleukin-6", Growth Horm IGF Res, 13(A), pp.28~32 (2003)
- Pedersen BK, "Exercise and cytokines with particular focus on muscle-derived IL-6", Immunol Rev, 7, pp.18~31 (2001)
- Marriott JB, "CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-α production", J Immunol, 161, pp.4236~4243 (1998)
- 민헌기, "임상내분비학", pp.667~ (1999), 고려의학
- Stiefel P, "Apolipoprotein E gene polymorphism is related to metabolic abnormalities, but does not influence erythrocyte membrane lipid composition or sodiumlithium counter transport activity in essential hypertension", Metabolism, 50, pp.157~160 (2001)
- Oh JY, "Apolipoprotein E polymorphism and lipid levels differ by gender and family history of diabetes. the Rancho Bernardo Study", Clin Genet, 60, pp.132~137 (2001)
- Hotamisligil GS, "Adipose expression of tumor necrosis factor-α: direct role in obesity-linked insulin resistance", Science, 259, pp.87~91 (1993)