황련해독탕 추출액의 각막염 원인균에 대한 항균효과
Antimicrobial Activity of Hwangryunhaedok-tang Extract against Keratitis
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Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of 70 μl dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from 70 μl to 10 μl at the same concentration. Results:1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show anyantimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentrationbut didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract 70 μl was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.
Abstract
Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of 70 μl dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from 70 μl to 10 μl at the same concentration. Results:1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show anyantimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentrationbut didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract 70 μl was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.
참고문헌 (34)
- McLeod SD, "The role of smears, cultures, and antibiotic sensitivity testing in the management of suspected infectious keratitis", Ophthalmology, 103(1), pp.23~28 (1996)
- Huang AJ, "Bacterial keratitis", pp.1025~1033 (2005), Elsevier Mosby
- Armstrong RA, "The microbiology of the eye", Ophthalmic Physiol Opt, 20(6), pp.429~441 (2000)
- Leck AK, "Aetiology of suppurative corneal ulcers in Ghana and south India, and epidemiology of fungal keratitis", Br J Ophthalmol, 86(11), pp.1211~1215 (2002)
- Hahn YH, "Antibiotic Susceptibilities of Ocular Isolates from Patients with Bacterial Keratitis. A Multi-center Study", J Korean Ophthalmol Soc, 40(9), pp.2401~2410 (1999)
- Jones DB, "Decision-making in the management of microbial keratitis", Ophthalmology, 88, pp.814~820 (1981)
- Liesegang TJ, "Bacterial keratitis", Infect Dis Clin North Am, pp.815~829 (1992)
- Afshari NA, "Trends in resistance to ciprofloxacin, cefazolin, and gentamicin in the treatment of bacterial keratitis", J Ocul Pharmaco Ther, 24(2), pp.217~223 (2008)
- Freidlin J, "Spectrum of eye disease caused by methicillin-resistant Staphylococcus aureus", Am J Ophthalmol, 144(2), pp.313~315 (2007)
- 최현태, "감염성 안질환의 원인균 및 항생제 감수성에 대한 10년간의 고찰", 대한안과학회지, 53(7), pp.934~940 (2012), 대한안과학회[DOI]
- 박주홍, "입원 치료를 시행한 감염각막염의 분석: 미생물학적 원인, 임상 양상 및 위험 인자", 대한안과학회지, 50(8), pp.1152~1166 (2009), 대한안과학회
- 노석선, "원색안이비인후과학", pp.160~165 (2007), (주)아이비씨기획
- 강은교, "점안용 소염약침액의 안전성 및 유효성 평가", Journal of Pharmacopuncture, 12(1), pp.21~33 (2009), 대한약침학회
- 김성원, "한방전문의총서 3권 안과", pp.71~150 (1996), 해동의학사
- 李傳課, "中醫眼科學", pp.477~479 (2002), 人民衛生出版社
- Choi GD, "The Effect of Coptidis Rhizoma, fraxini Cortex, Jinpisan on Experimental Pseudominas aeruginosa Keratitis", The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology, 12(1), pp.18~35 (1999)
- Lee SJ, "Three Cases of Pseudomonas Corneal Ulcer in Soft Contact Lens Wearer", Journal of The Korean Ophthalmological Society, 31(5), pp.681~686 (1990)
- Seo HS, "A Literatual Study on the external treatment of eye diseases", The Journal of Medical surgery, Ophthalmology &Otolaryngology, 10(1), pp.185~208 (1997)
- Lee JA, "Literatural study on Eye-drop", J Oriental Medical surgery, Ophthalmology & Otolaryngology, 6(1), pp.155~170 (1993)
- 정순영, "다종의 안질환 사용 처방이 각막염 유발균인 Staphylococcus epidermidis에 미치는 영향", 한방안이비인후피부과학회지, 19(1), pp.123~134 (2006), 대한한방안이비인후피부과학회
- Khosravi AD, "Bacteriological findings in patients with ocular infection and antibiotic susceptibility patterns of isolated pathogens", Singapore Med J, 48(8), pp.741~743 (2007)
- McClellan KA, "Mucosal defense of the outer eye", Surv of Ophthalmol, 42(3), pp.233~246 (1997)
- A Multi-center Study, "J Korean Ophthalmol Soc", 1998, 1633~1651
- 한영호, "감염성 각막염의 역학조사(II): 다병원연구", 대한안과학회지, 42(2), pp.247~265 (2001), 대한안과학회
- 김우진, "세균각막염의 예후인자 및 항생제 감수성 변화에 대한 8년간의 고찰", 대한안과학회지, 50(10), pp.1495~1504 (2009), 대한안과학회
- Afshari NA, "Trends in resistance to ciprofloxacin, cefazolin, and gentamicin in the treatment of bacterial keratitis", J Ocul Pharmacol Ther, 24(2), pp.217~223 (2008)
- Yilmaz S, "Microbial keratitis in West Anatolia, Turkey: a retrospective review", Int Ophthalmology, 27(4), pp.261~268 (2007)
- Wong T, "Severe infective keratitis leading to hospital admission in New Zealand", Br J Ophthalmol, 87(9), pp.1103~1108 (2003)
- 임수호, "입원치료를 시행한 세균각막염에 대한 12년간의 분석: 원인균주와 항생제내성", 대한안과학회지, 53(3), pp.372~384 (2012), 대한안과학회
- 서형식, "湯泡散이 Staphylococcus species(S. aureus, S. epidermidis)의배양일에 따른 항균효과의 지속성에 관한 연구", Journal of Pharmacopuncture, 10(2), pp.67~71 (2007), 대한약침학회
- 서형식, "秦皮散이 Staphylococcus species(S. aureus, S. epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구", Journal of Pharmacopuncture, 10(2), pp.87~91 (2007), 대한약침학회
- 서형식, "황련 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구", 한방안이비인후피부과학회지, 19(2), pp.71~76 (2006), 대한한방안이비인후피부과학회
- 이채우, "黃連解毒湯 약침요법을 통한 慢性 結膜炎 또는 眼球乾燥證 환자 1례에 대한 보고", Korean Journal of Acupuncture, 20(4), pp.7~84 (2003), 경락경혈학회
- 신민교, "임상본초학", pp.372~400 (2000), 영림사