돼지 브랜드 식별 및 원산지 추적에 활용 가능한 Microsatellite Marker Set의 확립
Establishment of a Microsatellite Marker Set for Individual, Pork Brand and Product Origin Identification in Pigs
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초록
돼지 원산지 추적 및 브랜드육 식별을 위한 MS marker set를 확립하기 위해 EU의 EID+DNA DNA Tracing 연구 project, 국제동물유전학회 및 Roslin 연구소 등에서 제안한 돼지의 다형성 분석에 사용되는 MS marker 중 17종을 선발하여 다형성지수, F-통계량, 동일개체 출현확률 및 친자감별확률 등을 MSA, CERVUS, FSTAT, GENEPOP 및 API-CALC 프로그램 등을 연계적으로 활용하여 추정하였다. 다형성지수 추정치를 1차 선발요인으로 하고, 각 MS marker의 PCR 결과물의 크기 및 분석 비용의 경제성을 추가로 감안하여 최종 13종의 MS marker(SW936, SW951, SW787, S00090, S0026, SW122, SW857, S0005, SW72, S0155, S0225, SW24 and SW632)와 성감별을 위한 2개의 성감별 primer로 조합된 하나의 Multiplexing PCR 시스템을 확립하였다. 이를 이용해 돼지 사육환경을 무작위 교배집단(PI), 반형매 교배집단 그리고 전형매 교배집단으로 가정하여 동일개체 출현확률을 분석한 결과 2.47×10－18, 6.39×10－13 Table2.Expected probability of identity(PI), among genotypes of random individuals, probability of identity among genotypes from random half sibs(PIhalf-sibs), among genotypes of random individuals, probability of identity among genotypes from random sibs(PIsibs), exclusionary power of the second parent(PE), and the probability of parent exclusion when both parents are unconfirmed(PEpu) No. of MS PI PIhalf-sibs PIsibs PEpu PE 17a 1.09×10-17 1.09×10-13 1.09×10-7 0.99974 1.00000 13b 2.47×10-18 6.39×10-13 1.08×10-8 0.999998 0.999669 a, the 17 MS markers in Table 1 and b, the 13 MS markers (SW936, SW951, SW787, S00090, S0026, SW122, SW857, S0005, SW72, S0155, S0225, SW24 and SW632) selected from the 17 MS markers by the rank of heterozygosity and PCR product size 그리고 1.08×10－8로 나타나 브랜드 식별 및 원산지 추적에 적합할 것으로 사료된다.
Abstract
Seventeen porcine microsatellite(MS) markers recommended by the EID+DNA Tracing EU project, ISAG and Roslin institute were selected for the use in porcine individual and brand identification. The MSA, CERVUS, FSTAT, GENEPOP and API-CALC programs were applied for calculating heterozygosity indices. By considering the hetreozygosity value and PCR product size of each marker, we established a MS marker set composed of 13 MS markers(SW936, SW951, SW787, S00090, S0026, SW122, SW857, S0005, SW72, S0155, S0225, SW24 and SW632) and two sexing markers. The expected probability of identity among genotypes of random individuals(PI), probability of identity among genotypes from random half sibs(PIhalf-sibs) and among genotypes of random individuals, probability of identity among genotypes from random sibs(PIsibs) were estimated as 2.47×10－18, 6.39×10－13 and 1.08×10－8, respectively. The results indicate that the established marker set can provide a sufficient discriminating power in both individual and parentage identification for the commercial pigs produced in Korea.
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